scholarly journals Detection and classification of paraproteins by capillary immunofixation/subtraction

1998 ◽  
Vol 44 (4) ◽  
pp. 760-764 ◽  
Author(s):  
Xavier Bossuyt ◽  
Ann Bogaerts ◽  
Gilberte Schiettekatte ◽  
Norbert Blanckaert
Keyword(s):  
Capillary Electrophoresis ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Detection And Identification ◽  
Agarose Electrophoresis ◽  
Oligoclonal Banding ◽  
Capillary Zone ◽  
Electrophoresis System ◽  
Selection Of

Abstract A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation and/or immunoelectrophoresis but not by capillary electrophoresis were also missed by agarose electrophoresis and cellulose acetate electrophoresis. Cellulose acetate electrophoresis was the least sensitive method for detection of paraproteins. Only 74% of the monoclonal components were detected by this technique, whereas 86% were revealed by agarose electrophoresis. In addition to monoclonal paraproteins, we also studied biclonal paraproteins and oligoclonal banding. Capillary electrophoresis and immunosubtraction correctly detected and identified three specimens containing biclonal paraproteins. In one specimen, capillary zone electrophoresis detected only one band, whereas agarose gel electrophoresis detected two bands. The sensitivity for detection and identification of oligoclonal banding by capillary electrophoresis was inferior to immunofixation.

scholarly journals Serum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system

1998 ◽  
Vol 44 (4) ◽  
pp. 749-759 ◽  
Author(s):  
Xavier Bossuyt ◽  
Gilberte Schiettekatte ◽  
Ann Bogaerts ◽  
Norbert Blanckaert
Keyword(s):  
Linear Correlation ◽  
Clinical Information ◽  
Turnaround Time ◽  
Reference Intervals ◽  
Cellulose Acetate Electrophoresis ◽  
Agarose Electrophoresis ◽  
Capillary Zone ◽  
Polyclonal Gammopathy ◽  
Electrophoresis System ◽  
Manual Methods

Abstract We compared the automated Paragon 2000 clinical capillary zone electrophoresis (CZE) system with two manual methods, agarose electrophoresis (AGE) and cellulose acetate electrophoresis (CAE). Reference intervals in healthy adults were determined for each method. When compared with AGE and CAE, CZE gave substantially higher reference values for the α1-globulin fraction. With CZE, within-run precision for fraction quantitation was between 0.5% (albumin) and 4.1% (α1-globulin). Total precision was between 0.8% (albumin) and 5.3% (β-globulin). Data obtained from CZE showed poor linear correlation with results obtained by AGE but good linear correlation with data from CAE. Analysis of serum from patients with inter alia inflammation, nephrotic syndrome, or polyclonal gammopathy showed that clinical information obtained by CZE is comparable with information obtained by AGE and CAE. We conclude that CZE offers a clinically reliable alternative to AGE and CAE and has the advantages of automation, higher precision, and faster turnaround time.

A portable allozyme electrophoresis kit used to identify members of the Simulium damnosum Theobald complex (Diptera: Simuliidae) in the field

1989 ◽  
Vol 79 (4) ◽  
pp. 685-691 ◽  
Author(s):  
M. C. Thomson ◽  
J. B. Davies ◽  
M. D. Wilson
Keyword(s):  
Cellulose Acetate ◽  
Sierra Leone ◽  
Electricity Supply ◽  
Allozyme Electrophoresis ◽  
Cellulose Acetate Electrophoresis ◽  
Simulium Damnosum ◽  
Field Laboratory ◽  
Run Off ◽  
Electrophoresis System

AbstractA cheap power pack that could be run off a 12-V car battery was constructed using a 12–250–V DC-DC converter. The power pack is small and light and can be used to run a Helena cellulose acetate electrophoresis system away from a mains electricity supply. The system was used sucessfully to identify adults of the Simulium damnosum Theobald complex in a field laboratory in northern Sierra Leone.

scholarly journals Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing

1998 ◽  
Vol 44 (3) ◽  
pp. 599-605 ◽  
Author(s):  
Jacques Bienvenu ◽  
Maria Stella Graziani ◽  
François Arpin ◽  
Hélène Bernon ◽  
Cynthia Blessum ◽  
...  
Keyword(s):  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Similar Data ◽  
Cellulose Acetate Electrophoresis ◽  
Zone Electrophoresis ◽  
Γ Globulins ◽  
Capillary Zone ◽  
Electrophoresis System ◽  
Monoclonal Component

Abstract Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZETM 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were <2% for albumin and γ-globulins and 4–7% for α1-, α2-, and β-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was <0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations >10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

scholarly journals Comparison of Capillary Electrophoresis with Cellulose Acetate Electrophoresis for the Screening of Hemoglobinopathies

2011 ◽  
Vol 31 (4) ◽  
pp. 238-243 ◽  
Author(s):  
Ji-Eun Kim ◽  
Bo-Ram Kim ◽  
Kwang-Sook Woo ◽  
Jeong-Man Kim ◽  
Joo-In Park ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis

Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

1965 ◽  
Vol 11 (10) ◽  
pp. 937-942 ◽  
Author(s):  
Alex Kaplan ◽  
John Savory
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Blood Donors ◽  
Serum Proteins ◽  
Normal Values ◽  
Protein Fractionation ◽  
Cellulose Acetate Electrophoresis ◽  
Cellulose Acetate Membranes ◽  
Control Serum ◽  
Electrophoresis System

Abstract A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the α1-, α2-, β-, and γ-globulin fractions. Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

High-resolution zone electrophoresis, combined with immunofixation, in the detection of an occult myeloma paraprotein.

1982 ◽  
Vol 28 (11) ◽  
pp. 2312-2313 ◽  
Author(s):  
C M Reichert ◽  
D F Everett ◽  
P I Nadler ◽  
N M Papadopoulos
Keyword(s):  
Multiple Myeloma ◽  
High Resolution ◽  
Recurrent Disease ◽  
Cellulose Acetate Electrophoresis ◽  
Myeloma Protein ◽  
Detection And Identification ◽  
Zone Electrophoresis ◽  
Agarose Electrophoresis ◽  
History Of ◽  
Initiation Of Therapy

Abstract A high-resolution agarose gel electrophoretic technique, coupled with immunofixation, was used to follow paraprotein concentrations retrospectively in a patient with multiple myeloma of nine years' duration. Although the patient's IgA lambda gammopathy "disappeared" shortly after the initiation of therapy, as judged by routine cellulose acetate electrophoresis and immunoelectrophoresis, high-resolution zone electrophoresis demonstrated a monoclonal band that we identified by immunofixation as the IgA lambda paraprotein. The combination of the two simple, inexpensive, and reliable techniques of high-resolution agarose electrophoresis and immunofixation thereby permitted detection and identification of a myeloma protein in a patient otherwise thought to be in complete remission. We believe this approach is useful in assessing persistent or recurrent disease in patients with a known history of myeloma; this combination of techniques may also prove beneficial in the early diagnosis of multiple myeloma.

scholarly journals CSF Electrophoresis in One Thousand Patients

1980 ◽  
Vol 7 (4) ◽  
pp. 275-280 ◽  
Author(s):  
G.C. Ebers ◽  
D.W. Paty
Keyword(s):  
Multiple Sclerosis ◽  
Immune Response ◽  
Cellulose Acetate ◽  
Diagnostic Criteria ◽  
Neurological Diseases ◽  
Local Immune Response ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. In patients with clinically definite multiple sclerosis (MS) (N = 267) 92.8% had oligoclonal banding (O.B.). In patients with possible MS (N - 283) O.B. was present in 31.1%. In patients with other neurological diseases (N = 450) O.B. was present in 8% (N = 36). Nineteen non-MS patients with positive O.B. had serum bands or disorders known to be associated with local immune response. The remaining 17 patients had no explanation for the oligoclonal banding. In the majority of these MS had not been a diagnostic consideration.CSF electrophoresis is the single most reliable laboratory lest in multiple sclerosis and deserves incorporation into the diagnostic criteria for the disease.

scholarly journals CSF Electrophoresis: An Adaptation Using Cellulose Acetate for the Identification of Oligoclonal Banding

1978 ◽  
Vol 5 (3) ◽  
pp. 297-299 ◽  
Author(s):  
D.W. Paty ◽  
M. Donnelly ◽  
M.E. Bernardo
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Definite Multiple Sclerosis ◽  
Oligoclonal Banding ◽  
Clinically Definite Multiple Sclerosis

SUMMARY:An adaptation of cellulose acetate electrophoresis for studying concentrated cerebrospinal fluid is described. Two hundred and twenty-one patients have been studied, and the specificity for multiple sclerosis and sub-acute sclerosing panencephalitis is discussed. This has been positive for oligoclonal banding (OB) in 79% of patients with clinically definite multiple sclerosis.

Determination of Serum Lactic Dehydrogenase Isoenzymes by Use of the "Diagnostest" Cellulose Acetate Electrophoresis System

1971 ◽  
Vol 17 (4) ◽  
pp. 326-331 ◽  
Author(s):  
John Di Giorgio
Keyword(s):  
Cellulose Acetate ◽  
Lactic Dehydrogenase ◽  
Phenazine Methosulfate ◽  
Normal Range ◽  
Cellulose Acetate Electrophoresis ◽  
Men And Women ◽  
Serum Lactic Dehydrogenase ◽  
Electrophoresis System ◽  
Entire Procedure

Abstract A method is described for measuring serum lactic dehydrogenase isoenzymes by use of a commercial electrophoresis system. Six samples are simultaneously electrophoresed on cellulose acetate plates to separate the five isoenzymes, which are made visible by overlaying and incubating the electrophoresed plate with another plate saturated with lactate, phenazine methosulfate, and INT (a tetrazolium dye). The pattern is qualitatively assessed, or the plates are made transparent with dimethylsulfoxide— propanol and the isoenzymes measured densitometrically. The normal range for each isoenzyme, determined on 39 nonmedicated, nonfasting men and women, agrees with reported values. Duplicate plates are obtained for each serum analyzed. The entire procedure requires 2 h.

Use of photodynamic therapy for photodamage skin (review of literature)

2019 ◽  
Vol 1 (7) ◽  
pp. 19-23
Author(s):  
S. I. Surkichin ◽  
N. V. Gryazeva ◽  
L. S. Kholupova ◽  
N. V. Bochkova
Keyword(s):  
Photodynamic Therapy ◽  
Comparative Evaluation ◽  
Clinical Manifestations ◽  
Light Sources ◽  
Review Of Literature ◽  
Photosensitizing Agents ◽  
Selection Of

The article provides an overview of the use of photodynamic therapy for photodamage of the skin. The causes, pathogenesis and clinical manifestations of skin photodamage are considered. The definition, principle of action of photodynamic therapy, including the sources of light used, the classification of photosensitizers and their main characteristics are given. Analyzed studies that show the effectiveness and comparative evaluation in the selection of various light sources and photosensitizing agents for photodynamic therapy in patients with clinical manifestations of photodamage.

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