Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor

2008 ◽  
Vol 80 (16) ◽  
pp. 6245-6252 ◽  
Author(s):  
Jonas Borch ◽  
Federico Torta ◽  
Stephen G. Sligar ◽  
Peter Roepstorff
Keyword(s):  
Kinetic Analysis ◽  
Cholera Toxin ◽  
Lipid Bilayers ◽  
Membrane Receptors ◽  
Toxin Binding ◽  
Glycolipid Receptor

α-Adrenergic receptors regulate human lymphocyte amiloride-sensitive sodium channels

1998 ◽  
Vol 275 (3) ◽  
pp. C702-C710 ◽  
Author(s):  
James K. Bubien ◽  
Trudy Cornwell ◽  
Anne Lynn Bradford ◽  
Catherine M. Fuller ◽  
Michael D. DuVall ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Sodium Channels ◽  
Adrenergic Receptors ◽  
Binding Proteins ◽  
Second Messengers ◽  
Specific Inhibitor ◽  
Direct Interaction ◽  
Membrane Receptors ◽  
Gtp Binding Proteins ◽  
Gtp Binding

Two independent signal transduction pathways regulate lymphocyte amiloride-sensitive sodium channels (ASSCs), one utilizing cAMP as a second messenger and the other utilizing a GTP-binding protein. This implies that two plasma membrane receptors play a role in the regulation of lymphocyte ASSCs. In this study, we tested the hypothesis that α1- and α2-adrenergic receptors independently regulate lymphocyte ASSCs via the two previously identified second messengers. Direct measurements indicated that norepinephrine increased lymphocyte cAMP and activated ASSCs. The α2-specific inhibitor, yohimbine, blocked this activation, thereby linking α2-adrenergic receptors to ASSC regulation via cAMP. The α1-specific ligand, terazosin, acted as an agonist and activated lymphocyte ASSCs but inhibited ASSC current that had been preactivated by norepinephrine or 8-(4-chlorophenylthio) (CPT)-cAMP. Terazosin had no effect on the lymphocyte whole cell ASSC currents preactivated by treatment with pertussis toxin. This finding indirectly links α1-adrenergic receptors to lymphocyte ASSC regulation via GTP-binding proteins. Terazosin had no direct inhibitory or stimulatory effects on α,β,γ-endothelial sodium channels reconstituted into planar lipid bilayers and expressed in Xenopus oocytes, ruling out a direct interaction between terazosin and the channels. These findings support the hypothesis that both α1- and α2-adrenergic receptors independently regulate lymphocyte ASSCs via GTP-binding proteins and cAMP, respectively.

Short Term Neonatal Starvation Altered Cholera Toxin Binding in Rabbits

1989 ◽  
Vol 119 (2) ◽  
pp. 280-285
Author(s):  
Susan S. Baker ◽  
Cara Campbell ◽  
W. Allan Walker
Keyword(s):  
Cholera Toxin ◽  
Short Term ◽  
Toxin Binding

Identification of cholera toxin-binding sites in the nucleus of intestinal epithelial cells

FEBS Letters ◽  
1989 ◽  
Vol 242 (2) ◽  
pp. 309-313 ◽  
Author(s):  
Marianne E. Parkinson ◽  
Colin G. Smith ◽  
Peter B. Garland ◽  
Simon van Heyningen
Keyword(s):  
Epithelial Cells ◽  
Cholera Toxin ◽  
Binding Sites ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Toxin Binding

Strong inhibition of cholera toxin binding by galactose dendrimers

2007 ◽  
pp. 5043 ◽  
Author(s):  
Hilbert M. Branderhorst ◽  
Rob M. J. Liskamp ◽  
Gerben M. Visser ◽  
Roland J. Pieters
Keyword(s):  
Cholera Toxin ◽  
Strong Inhibition ◽  
Toxin Binding
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