Exploring the Precursor Ion Exclusion Feature of Liquid Chromatography−Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry for Improving Protein Identification in Shotgun Proteome Analysis

2008 ◽  
Vol 80 (12) ◽  
pp. 4696-4710 ◽  
Author(s):  
Nan Wang ◽  
Liang Li
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Protein Identification ◽  
Time Of Flight ◽  
Proteome Analysis ◽  
Flight Mass Spectrometry ◽  
Ion Exclusion

scholarly journals Metabolic profiling putatively identifies plasma biomarkers of male infertility using UPLC-ESI-IT-TOFMS

RSC Advances ◽  
2018 ◽  
Vol 8 (46) ◽  
pp. 25974-25982 ◽  
Author(s):  
F. J. Zeng ◽  
H. C. Ji ◽  
Z. M. Zhang ◽  
J. K. Luo ◽  
H. M. Lu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Male Infertility ◽  
Electrospray Ionization ◽  
Metabolic Profiling ◽  
Ion Trap ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Potential Biomarkers

Ultra-performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry combined with chemometrics methods was used to discover potential biomarkers of male infertility based on untargeted plasma metabolomics.

Rapid Detection and Characterization of Steroidal Saponins in the Root of Asparagus cochinchinensis by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

2020 ◽  
Vol 58 (5) ◽  
pp. 454-463
Author(s):  
Yun Ling ◽  
Xin He ◽  
Renliang Jiang ◽  
Qi Zhang ◽  
Shuqin Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
High Performance ◽  
Time Of Flight ◽  
Steroidal Saponins ◽  
Flight Mass Spectrometry ◽  
Asparagus Cochinchinensis

Abstract The dried root of Asparagus cochinchinensis (RAC) has been used as an important traditional Chinese medicine for a long time in China. Steroidal saponins (SSs) are considered to be the main active ingredients of this herb. However, the isolation and structural determination of SSs from RAC are time-consuming and laborious. For this reason, the development of new methods for the separation and characterization of SSs is highly desirable. In this study, a new high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) method with precursor ions and the corresponding fragment ions was developed for the identification of SSs in RAC. Finally, 30 SSs have been detected and identified, including 17 potential new compounds. This is the first systematic study of SSs in RAC by HPLC-ESI-QTOF-MS/MS method.

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