Lectin-Based Nanoprobes Functionalized with Enzyme for Highly Sensitive Electrochemical Monitoring of Dynamic Carbohydrate Expression on Living Cells

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

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A highly sensitive and selective fluorescent chemosensor for the sequential recognition of Zn2+ and S2− in living cells and aqueous media

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2017.09.136 ◽

2018 ◽

Vol 255 ◽

pp. 3108-3116 ◽

Cited By ~ 20

Author(s):

Jin Yeong Yun ◽

Tae Geun Jo ◽

Jiyeon Han ◽

Hyo Jung Jang ◽

Mi Hee Lim ◽

...

Keyword(s):

Aqueous Media ◽

Living Cells ◽

Fluorescent Chemosensor ◽

Highly Sensitive ◽

Sequential Recognition

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A highly-sensitive “turn on” probe based on coumarin β-diketone for hydrazine detection in PBS and living cells

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2021.119510 ◽

2021 ◽

Vol 252 ◽

pp. 119510

Author(s):

Rui Chen ◽

Guang-Jin Shi ◽

Jia-Jia Wang ◽

Hai-Feng Qin ◽

Qi Zhang ◽

...

Keyword(s):

Living Cells ◽

Turn On ◽

Highly Sensitive

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Highly sensitive real-time detection of intracellular oxidative stress and application in mycotoxin toxicity evaluation based on living single-cell electrochemical sensors

The Analyst ◽

10.1039/d0an02015j ◽

2021 ◽

Author(s):

Lu Gao ◽

Jiadi Sun ◽

Liping Wang ◽

Qigao Fan ◽

Gaowen Zhu ◽

...

Keyword(s):

Oxidative Stress ◽

Electrochemical Sensor ◽

Single Cell ◽

Real Time ◽

Electrochemical Sensors ◽

Living Cells ◽

Selective Detection ◽

Toxicity Evaluation ◽

Highly Sensitive ◽

Intracellular Oxidative Stress

Single-cell electrochemical sensor is used in the local selective detection of living cells because of its high spatial–temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes.

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Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode

The Analyst ◽

10.1039/d1an00220a ◽

2021 ◽

Author(s):

Jing Zhou ◽

Dan Yang ◽

Guohui Liu ◽

Siying Li ◽

Wennan Feng ◽

...

Keyword(s):

Dna Damage ◽

Oxidative Damage ◽

Living Cells ◽

Oxidation Products ◽

Electrochemical Measurements ◽

Sensitive Detection ◽

Dna Oxidative Damage ◽

Highly Sensitive ◽

Highly Sensitive Detection ◽

Gold Nanoelectrode

Guanine (G) oxidation products, such as 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 8-oxo-guanine (8-OXOG), have been widely studied as promising biomarkers for DNA oxidative damage.

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A Novel Highly Sensitive Dual-channel Chemical Sensor for Sequential Recognition of Cu2+ and CN- in Aqueous Media and its Bioimaging Applications on Living Cells

New Journal of Chemistry ◽

10.1039/d1nj03548g ◽

2021 ◽

Author(s):

Yong-Qiang Xie ◽

You-Ming Zhang ◽

Zhao-Hui Li ◽

Qi Xiao-Ni ◽

Hong Yao ◽

...

Keyword(s):

Chemical Sensor ◽

Aqueous Media ◽

Living Cells ◽

Chemical Probe ◽

Dual Channel ◽

Highly Sensitive ◽

Sequential Recognition

A simple and unique dual-channel chemical probe (DH) was designed and synthesized, which not only realized sequential recognition of Cu2+ and CN− by colorimetric and fluorometric methods, but also realized...

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