scholarly journals Poly(acrylamide-co-N,N′-methylenebisacrylamide) Monoliths for High-Peak-Capacity Hydrophilic-Interaction Chromatography–High-Resolution Mass Spectrometry of Intact Proteins at Low Trifluoroacetic Acid Content

2021 ◽  
Author(s):  
Marta Passamonti ◽  
Chiem de Roos ◽  
Peter J. Schoenmakers ◽  
Andrea F.G. Gargano
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Trifluoroacetic Acid ◽  
Acid Content ◽  
High Resolution Mass Spectrometry ◽  
Peak Capacity ◽  
Hydrophilic Interaction ◽  
Intact Proteins ◽  
Resolution Mass ◽  
Poly Acrylamide

scholarly journals N-Glycosylation profiling of intact target proteins by high-resolution mass spectrometry (MS) and glycan analysis using ion mobility-MS/MS

The Analyst ◽  
2020 ◽  
Vol 145 (5) ◽  
pp. 1737-1748
Author(s):  
Alessandro Quaranta ◽  
Maya Spasova ◽  
Elena Passarini ◽  
Isabella Karlsson ◽  
Lorena Ndreu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
High Resolution ◽  
Ion Mobility ◽  
High Resolution Mass Spectrometry ◽  
Target Proteins ◽  
Intact Proteins ◽  
Glycan Analysis ◽  
High Resolution Mass ◽  
Resolution Mass

Glycosylation characterization could lead to the discovery of biomarkers and is crucial in quality control of biopharmaceuticals. Here we present a method to quantify glycoforms on intact proteins, with parallel glycan identification by IMS-MS/MS.

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