Electrochemical Detection of Circulating Tumor Cells Based on DNA Generated Electrochemical Current and Rolling Circle Amplification

2019 ◽  
Vol 91 (18) ◽  
pp. 11614-11619 ◽  
Author(s):  
Congcong Shen ◽  
Shuping Liu ◽  
Xiaoqing Li ◽  
Minghui Yang
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Electrochemical Detection ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Electrochemical Current

Highly Sensitive Electrochemical Biosensor for Circulating Tumor Cells Detection via Dual-Aptamer Capture and Rolling Circle Amplification Strategy

2019 ◽  
Vol 15 (7) ◽  
pp. 1568-1577 ◽  
Author(s):  
Yang Wang ◽  
Kai Chang ◽  
Cheng Yang ◽  
Shujing Li ◽  
Lixin Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
K562 Cells ◽  
Detection Sensitivity ◽  
Experimental Conditions ◽  
Simple Method ◽  
Rolling Circle

A fast and simple strategy for early detection of circulating tumor cells (CTCs) is urgently required because of cancer metastasis. In this work, we assembled an electrochemical biosensor by two aptamers that could form hairpin and specifically recognize K562 cells. The thiolated capture aptamer was fixed on the gold electrode surface. The detection aptamer was linked with a primer at 3 end which could trigger rolling circle amplification to prolong the sequence of aptamer. The dual-aptamer model was fabricated to improve the capture specificity and efficiency for K562 cells. The rolling circle amplification improved the detection sensitivity by inhibiting electron transfer of [Fe(CN)6]3–/4– which could be measured by differential pulse voltammetry. The detection limit of 25 cells mL–1 and linear ranges of 1 × 10 2 to 1 × 105 cells mL–1 were obtained under optimal experimental conditions. Our work exhibited a label-free and simple method for detecting CTCs using cell-specific aptasensor, showing an expected possibility for further CTCs-related study and clinical applications of this novel method.

DNA-mediated reversible capture and release of circulating tumor cells with a multivalent dual-specific aptamer coating network

2019 ◽  
Vol 55 (37) ◽  
pp. 5387-5390 ◽  
Author(s):  
Yuhong Lin ◽  
Lili Jiang ◽  
Yuqing Huang ◽  
Yuling Yang ◽  
Yu He ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Capture And Release

Reversible isolation and release of circulating tumor cells based on DNA-responsive multivalent dual-aptamer-tethered rolling circle amplification network.

Aptamer-Based Rolling Circle Amplification:  A Platform for Electrochemical Detection of Protein

2007 ◽  
Vol 79 (19) ◽  
pp. 7492-7500 ◽  
Author(s):  
Long Zhou ◽  
Li-Juan Ou ◽  
Xia Chu ◽  
Guo-Li Shen ◽  
Ru-Qin Yu
Keyword(s):  
Electrochemical Detection ◽  
Rolling Circle Amplification ◽  
Rolling Circle

Intracellular self-enhanced rolling circle amplification to image specific miRNAs within tumor cells

2019 ◽  
Vol 282 ◽  
pp. 507-514 ◽  
Author(s):  
Yan-Ru Chen ◽  
Ying Han ◽  
Ran Liu ◽  
Chang Xue ◽  
Huo Xu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Rolling Circle Amplification ◽  
Rolling Circle
Sign in / Sign up

Export Citation Format

Share Document