A Highly Sensitive Target-Primed Rolling Circle Amplification (TPRCA) Method for Fluorescent in Situ Hybridization Detection of MicroRNA in Tumor Cells

2014 ◽  
Vol 86 (3) ◽  
pp. 1808-1815 ◽  
Author(s):  
Jia Ge ◽  
Liang-Liang Zhang ◽  
Si-Jia Liu ◽  
Ru-Qin Yu ◽  
Xia Chu
Keyword(s):  
In Situ Hybridization ◽  
Tumor Cells ◽  
Fluorescent In Situ Hybridization ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Hybridization Detection

A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells

2016 ◽  
Vol 52 (2) ◽  
pp. 370-373 ◽  
Author(s):  
Jin Huang ◽  
He Wang ◽  
Xiaohai Yang ◽  
Yanjing Yang ◽  
Ke Quan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Signal Amplification ◽  
Fluorescence Signal ◽  
Target Mrna ◽  
Highly Sensitive ◽  
Fluorescence Signal Amplification

This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in thein situfluorescence signal amplification.

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

Fluorescent in situ hybridization (FISH) as a tool to identify chromosomal aberrations in human tumor cells

1993 ◽  
Vol 66 (2) ◽  
pp. 157
Author(s):  
Rachel A. Jesudasan ◽  
M. Rezaur Rahman ◽  
K.L. Ying ◽  
C. Patrick Reynolds ◽  
Eri S. Srivatsan
Keyword(s):  
In Situ Hybridization ◽  
Tumor Cells ◽  
Chromosomal Aberrations ◽  
Fluorescent In Situ Hybridization ◽  
Human Tumor ◽  
Human Tumor Cells

scholarly journals Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-Fluorescence In Situ Hybridization

2021 ◽  
Vol 9 (6) ◽  
pp. 1208
Author(s):  
Kyohei Horio ◽  
Hirokazu Takahashi ◽  
Toshiro Kobori ◽  
Kenshi Watanabe ◽  
Tsunehiro Aki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lactic Acid ◽  
In Situ Hybridization ◽  
Lactic Acid Bacteria ◽  
Fluorescence In Situ Hybridization ◽  
Rolling Circle Amplification ◽  
Rnase H ◽  
Microbial Interactions ◽  
Rolling Circle

Recently, we developed an in situ mRNA detection method termed RNase H-assisted rolling circle amplification-fluorescence in situ hybridization (RHa-RCA-FISH), which can detect even short mRNA in a bacterial cell. However, because this FISH method is sensitive to the sample condition, it is necessary to find a suitable cell permeabilization and collection protocol. Here, we demonstrate its further applicability for detecting intrinsic mRNA expression using lactic acid bacteria (LAB) as a model consortium. Our results show that this method can visualize functional gene expression in LAB cells and can be used for monitoring the temporal transition of gene expression. In addition, we also confirmed that data obtained from bulk analyses such as RNA-seq or microarray do not always correspond to gene expression in individual cells. RHa-RCA-FISH will be a powerful tool to compensate for insufficient data from metatranscriptome analyses while clarifying the carriers of function in microbial consortia. By extending this technique to capture spatiotemporal microbial gene expression at the single-cell level, it will be able to characterize microbial interactions in phytoplankton–bacteria interactions.

Fluorescent in situ Hybridization Detection of HER-2/neu Gene Amplification in Rhabdomyosarcoma

Pathobiology ◽  
1998 ◽  
Vol 66 (2) ◽  
pp. 59-63 ◽  
Author(s):  
Hon Fong L. Mark ◽  
Stephen Brown ◽  
Ci-Lin Sun ◽  
Mangala Samy ◽  
Alaa Afify
Keyword(s):  
In Situ Hybridization ◽  
Gene Amplification ◽  
Fluorescent In Situ Hybridization ◽  
Her 2 ◽  
Hybridization Detection
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