Quantification of the Mean and Distribution of Hemoglobin Content in Normal Human Blood Using Cell Tracking Velocimetry

2019 ◽  
Vol 92 (2) ◽  
pp. 1956-1962 ◽  
Author(s):  
James Kim ◽  
Jenifer Gómez-Pastora ◽  
Christopher J. Gilbert ◽  
Mitchell Weigand ◽  
Nicole A. Walters ◽  
...  
1977 ◽  
Vol 252 (7) ◽  
pp. 2331-2337 ◽  
Author(s):  
R M Winslow ◽  
M L Swenberg ◽  
R L Berger ◽  
R I Shrager ◽  
M Luzzana ◽  
...  

1956 ◽  
Vol 218 (1) ◽  
pp. 255-259
Author(s):  
John D. Evans ◽  
Jerome M. Waldron ◽  
Nadia L. Oleksyshyn ◽  
Roy W. Riemenschneider

1969 ◽  
Vol 26 (1) ◽  
pp. 1-3 ◽  
Author(s):  
E W Merrill ◽  
C S Cheng ◽  
G A Pelletier

Author(s):  
Jenifer Gómez-Pastora ◽  
James Kim ◽  
Victor Multanen ◽  
Mitchell Weigand ◽  
Nicole Walters ◽  
...  

The presence of iron in circulating monocytes is well known as they play an essential role in iron recycling. It has been demonstrated that the iron content of blood cells can be measured through their magnetic behavior; however, the magnetic properties of different monocyte subtypes remain unknown. In this study, we report for the first time, the magnetic behavior of classical, intermediate and non-classical monocytes, which is related to their iron storage capacity. The magnetic properties of monocytes were compared to other blood cells, such as lymphocytes and red blood cells in the oxyhemoglobin and methemoglobin states, and a cancer cell type. For this analysis, we used an instrument referred to as Cell Tracking Velocimetry (CTV), which quantitatively characterizes the magnetic behavior of biological entities. Our results demonstrate that significant fractions of the intermediate and non-classical monocytes have high magnetophoretic mobilities, equivalent to methemoglobin red blood cells and higher than the classical subset, suggesting their higher iron storage capacities. Moreover, our findings have implications for the immunomagnetic separation industry; we demonstrate that negative magnetic isolation techniques for recovering monocytes from blood should be used with caution, as it is possible to lose magnetic monocytes when using this technique.


Nature ◽  
1967 ◽  
Vol 215 (5109) ◽  
pp. 1483-1485 ◽  
Author(s):  
SOSAMMA JOHN ◽  
NATHAN BERGER ◽  
MARY JO BONNER ◽  
JULIUS SCHULTZ

Sign in / Sign up

Export Citation Format

Share Document