Investigating the Atomic and Mesoscale Interactions that Facilitate Spider Silk Protein Pre-Assembly

2021 ◽  
Author(s):  
David Onofrei ◽  
Dillan Stengel ◽  
Di Jia ◽  
Hannah R. Johnson ◽  
Samantha Trescott ◽  
...  
Keyword(s):  
Spider Silk ◽  
Silk Protein ◽  
Spider Silk Protein

scholarly journals Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 511
Author(s):  
Yu Suzuki ◽  
Takanori Higashi ◽  
Takahiro Yamamoto ◽  
Hideyasu Okamura ◽  
Takehiro K. Sato ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Formic Acid ◽  
Spider Silk ◽  
Chemical Shifts ◽  
Repetitive Sequences ◽  
Silk Protein ◽  
Random Coil ◽  
Oh Groups ◽  
Spider Silk Protein ◽  
Turn Structure

Spider dragline silk is a biopolymer with excellent mechanical properties. The development of recombinant spider silk protein (RSP)-based materials with these properties is desirable. Formic acid (FA) is a spinning solvent for regenerated Bombyx mori silk fiber with excellent mechanical properties. To use FA as a spinning solvent for RSP with the sequence of major ampullate spider silk protein from Araneus diadematus, we determined the conformation of RSP in FA using solution NMR to determine the role of FA as a spinning solvent. We assigned 1H, 13C, and 15N chemical shifts to 32-residue repetitive sequences, including polyAla and Gly-rich regions of RSP. Chemical shift evaluation revealed that RSP is in mainly random coil conformation with partially type II β-turn structure in the Gly-Pro-Gly-X motifs of the Gly-rich region in FA, which was confirmed by the 15N NOE data. In addition, formylation at the Ser OH groups occurred in FA. Furthermore, we evaluated the conformation of the as-cast film of RSP dissolved in FA using solid-state NMR and found that β-sheet structure was predominantly formed.

scholarly journals Interfacial Behavior of Recombinant Spider Silk Protein Parts Reveals Cues on the Silk Assembly Mechanism

Langmuir ◽  
2018 ◽  
Vol 34 (39) ◽  
pp. 11795-11805 ◽  
Author(s):  
Linnea Nilebäck ◽  
Suvi Arola ◽  
Mathias Kvick ◽  
Arja Paananen ◽  
Markus B. Linder ◽  
...  
Keyword(s):  
Spider Silk ◽  
Silk Protein ◽  
Interfacial Behavior ◽  
Assembly Mechanism ◽  
Spider Silk Protein

Glycopolymer Functionalization of Engineered Spider Silk Protein-based Materials for Improved Cell Adhesion

2014 ◽  
Vol 14 (7) ◽  
pp. 936-942 ◽  
Author(s):  
John G. Hardy ◽  
André Pfaff ◽  
Aldo Leal-Egaña ◽  
Axel H. E. Müller ◽  
Thomas R. Scheibel
Keyword(s):  
Cell Adhesion ◽  
Spider Silk ◽  
Silk Protein ◽  
Spider Silk Protein

Interactions of Fibroblasts with Different Morphologies Made of an Engineered Spider Silk Protein

2011 ◽  
Vol 14 (3) ◽  
pp. B67-B75 ◽  
Author(s):  
Aldo Leal-Egaña ◽  
Gregor Lang ◽  
Carolin Mauerer ◽  
Jasmin Wickinghoff ◽  
Michael Weber ◽  
...  
Keyword(s):  
Spider Silk ◽  
Silk Protein ◽  
Spider Silk Protein

Surface Features of Recombinant Spider Silk Protein eADF4(κ16)‐Made Materials are Well‐Suited for Cardiac Tissue Engineering

2017 ◽  
Vol 27 (36) ◽  
pp. 1701427 ◽  
Author(s):  
Jana Petzold ◽  
Tamara B. Aigner ◽  
Filip Touska ◽  
Katharina Zimmermann ◽  
Thomas Scheibel ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Protein ◽  
Surface Features ◽  
Spider Silk Protein

An Engineered Spider Silk Protein Forms Microspheres

2008 ◽  
Vol 47 (24) ◽  
pp. 4592-4594 ◽  
Author(s):  
Ute K. Slotta ◽  
Sebastian Rammensee ◽  
Stanislav Gorb ◽  
Thomas Scheibel
Keyword(s):  
Spider Silk ◽  
Silk Protein ◽  
Spider Silk Protein

Elastic theory of single spider silk protein molecule

2003 ◽  
Vol 438-439 ◽  
pp. 382-385 ◽  
Author(s):  
Luru Dai ◽  
Yong Zhang ◽  
Zhong-can Ou-Yang
Keyword(s):  
Spider Silk ◽  
Protein Molecule ◽  
Silk Protein ◽  
Elastic Theory ◽  
Spider Silk Protein

scholarly journals Spider silk protein structure analysis by FTIR and STXM spectromicroscopy techniques

2014 ◽  
Vol 2014 (1) ◽  
pp. 35-42 ◽  
Author(s):  
J. Normandeau ◽  
C. Van Kessel ◽  
D. Nicholson ◽  
B. Rahusaar Routledge ◽  
A. Fawcett ◽  
...  
Keyword(s):  
Protein Structure ◽  
Structure Analysis ◽  
Spider Silk ◽  
Silk Protein ◽  
Protein Structure Analysis ◽  
Spider Silk Protein
Sign in / Sign up

Export Citation Format

Share Document