Development of Liver Decellularized Extracellular Matrix Bioink for Three-Dimensional Cell Printing-Based Liver Tissue Engineering

2017 ◽  
Vol 18 (4) ◽  
pp. 1229-1237 ◽  
Author(s):  
Hyungseok Lee ◽  
Wonil Han ◽  
Hyeonji Kim ◽  
Dong-Heon Ha ◽  
Jinah Jang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Cell Printing ◽  
Decellularized Extracellular Matrix ◽  
Liver Tissue Engineering ◽  
Dimensional Cell

scholarly journals Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Birong Wang ◽  
Qinggang Hu ◽  
Tao Wan ◽  
Fengxiao Yang ◽  
Le Cui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Sessile Drop ◽  
Mechanical Stability ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Sem Images ◽  
Hemolysis Assay ◽  
Modified Chitosan ◽  
Liver Tissue Engineering

Galactosylated chitosan (CTS) has been widely applied in liver tissue engineering as scaffold. However, the influence of degree of substitution (DS) of galactose moieties on cell attachment and mechanical stability is not clear. In this study, we synthesized the lactose-modified chitosan (Lact-CTS) with various DS of galactose moieties by Schiff base reaction and reducing action of NaBH4, characterized by FTIR. The DS of Lact-CTS-1, Lact-CTS-2, and Lact-CTS-3 was 19.66%, 48.62%, and 66.21% through the method of potentiometric titration. The cell attachment of hepatocytes on the CTS and Lact-CTS films was enhanced accompanied with the increase of galactose moieties on CTS chain because of the galactose ligand-receptor recognition; however, the mechanical stability of Lact-CTS-3 was reduced contributing to the extravagant hydrophilicity, which was proved using the sessile drop method. Then, the three-dimensional Lact-CTS scaffolds were fabricated by freezing-drying technique. The SEM images revealed the homogeneous pore bearing the favorable connectivity and the pore sizes of scaffolds with majority of 100 μm; however, the extract solution of Lact-CTS-3 scaffold significantly damaged red blood cells by hemolysis assay, indicating that exorbitant DS of Lact-CTS-3 decreased the mechanical stability and increased the toxicity. To sum up, the Lact-CTS-2 with 48.62% of galactose moieties could facilitate the cell attachment and possess great biocompatibility and mechanical stability, indicating that Lact-CTS-2 was a promising material for liver tissue engineering.

Vasculature Formation Using Three-Dimensional Cell Printing Technology

2010 ◽  
Author(s):  
Vivian Lee ◽  
Guohao Dai
Keyword(s):  
Tissue Engineering ◽  
Vascular System ◽  
Three Dimensional ◽  
Clinical Applications ◽  
Growth And Survival ◽  
Cell Printing ◽  
Tissue Construct ◽  
Dimensional Cell ◽  
And Cartilage ◽  
Diffusion Problems

One of the major challenges in tissue engineering is vascularization which provides adequate supplies of oxygen and nutrients to cells within thick tissue-engineered constructs. Oxygen, nutrients and other molecules required for cell growth and survival can only diffuse to 150∼200 μm without proper vascular system. For this reason, thicker tissues have diffusion problems and cannot survive/proliferate well. To date, fabrications of relatively thin tissues such as skin and bladder, and cartilage tissues, which require low level of oxygen and nutrients, are reported. Obstacles in vascularization still exist for thick and complex tissue construct such as kidney, lung and heart (1). Overcoming this problem is a critical step to the clinical applications of tissue engineering (2).

scholarly journals Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1762 ◽  
Author(s):  
Juan Cui ◽  
Huaping Wang ◽  
Qing Shi ◽  
Tao Sun ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Liver Function ◽  
Cell Viability ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Cell Types ◽  
Liver Tissue Engineering ◽  
Multiple Cell

Three-dimensional (3D) tissue models replicating liver architectures and functions are increasingly being needed for regenerative medicine. However, traditional studies are focused on establishing 2D environments for hepatocytes culture since it is challenging to recreate biodegradable 3D tissue-like architecture at a micro scale by using hydrogels. In this paper, we utilized a gelatin methacryloyl (GelMA) hydrogel as a matrix to construct 3D lobule-like microtissues for co-culture of hepatocytes and fibroblasts. GelMA hydrogel with high cytocompatibility and high structural fidelity was determined to fabricate hepatocytes encapsulated micromodules with central radial-type hole by photo-crosslinking through a digital micromirror device (DMD)-based microfluidic channel. The cellular micromodules were assembled through non-contact pick-up strategy relying on local fluid-based micromanipulation. Then the assembled micromodules were coated with fibroblast-laden GelMA, subsequently irradiated by ultraviolet for integration of the 3D lobule-like microtissues encapsulating multiple cell types. With long-term co-culture, the 3D lobule-like microtissues encapsulating hepatocytes and fibroblasts maintained over 90% cell viability. The liver function of albumin secretion was enhanced for the co-cultured 3D microtissues compared to the 3D microtissues encapsulating only hepatocytes. Experimental results demonstrated that 3D lobule-like microtissues fabricated by GelMA hydrogels capable of multicellular co-culture with high cell viability and liver function, which have huge potential for liver tissue engineering and regenerative medicine applications.

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