scholarly journals Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

10.3791/54331 ◽  
2016 ◽  
Author(s):  
Hitomi Shirahama ◽  
Supriya K. Kumar ◽  
Won-Yong Jeon ◽  
Myung Hee Kim ◽  
Jae Ho Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Ethylene Glycol ◽  
Liver Tissue ◽  
Colloidal Crystal ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Liver Tissue Engineering ◽  
Poly Ethylene ◽  
Dimensional Cell

Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)

2013 ◽  
Vol 176 ◽  
pp. 1081-1089 ◽  
Author(s):  
Shilpa Sivashankar ◽  
Srinivasu Valegerahally Puttaswamy ◽  
Ling-Hui Lin ◽  
Tz-Shuian Dai ◽  
Chau-Ting Yeh ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Ethylene Glycol ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Tissue Slices ◽  
Glycol Diacrylate ◽  
Poly Ethylene ◽  
Mice Liver

scholarly journals Three-Dimensional Microassembly of Cell-Laden Microplates by in situ Gluing with Photocurable Hydrogels

2014 ◽  
Vol 8 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Shotaro Yoshida ◽  
◽  
Koji Sato ◽  
Shoji Takeuchi
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Interaction Analysis ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Poly Ethylene Glycol ◽  
Peg Hydrogels ◽  
Assembly Method ◽  
Poly Ethylene

This paper describes a method for assembling cellladen microplates into three-dimensional (3D) microstructures by in situ gluing using photocurable hydrogels. We picked up cell-laden microplates with microtweezers, placed the plate perpendicular to one another on a microgroove device, and glued them by local photopolymerization of biocompatible Poly (Ethylene Glycol) (PEG) hydrogels. The advantage of this assembly method is its ability to construct 3D biological microstructures with targeted cells. We demonstrated the assembly of a 3D half-cube microstructure with genetically labeled cell-laden microplates. We believe our method is useful for engineering the positions of cells in 3D configurations for cell-cell interaction analysis and tissue engineering.

The Mechanical Behavior of Engineered Hydrogels

2009 ◽  
Author(s):  
Audrey L. Earnshaw ◽  
Justine J. Roberts ◽  
Garret D. Nicodemus ◽  
Stephanie J. Bryant ◽  
Virginia L. Ferguson
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
A Chain

Agarose and poly(ethylene-glycol) (PEG) are commonly used as scaffolds for cell and tissue engineering applications [1]. Agarose is a natural biomaterial that is thought to be inert [2] and permits growing cells and tissues in a three-dimensional suspension [3]. Gels synthesized from photoreactive poly(ethylene glycol) (PEG) macromonomers are well suited as cell carriers because they can be rapidly photopolymerized in vivo by a chain radical polymerization that is not toxic to cells, including chondrocytes. This paper explores the differences in mechanical behavior between agarose, a physically cross-linked hydrogel, and PEG, a chemically cross-linked hydrogel, to set the foundation for choosing hydrogel properties and chemistries for a desired tissue engineering application.

scholarly journals Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks

2020 ◽  
Vol 6 (38) ◽  
pp. eabc5529 ◽  
Author(s):  
Liliang Ouyang ◽  
James P. K. Armstrong ◽  
Yiyang Lin ◽  
Jonathan P. Wojciechowski ◽  
Charlotte Lee-Reeves ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Synthetic Polymers ◽  
3D Bioprinting ◽  
Poly Ethylene Glycol ◽  
Encapsulated Cells ◽  
Heterogeneous Structures ◽  
Poly Ethylene

A major challenge in three-dimensional (3D) bioprinting is the limited number of bioinks that fulfill the physicochemical requirements of printing while also providing a desirable environment for encapsulated cells. Here, we address this limitation by temporarily stabilizing bioinks with a complementary thermo-reversible gelatin network. This strategy enables the effective printing of biomaterials that would typically not meet printing requirements, with instrument parameters and structural output largely independent of the base biomaterial. This approach is demonstrated across a library of photocrosslinkable bioinks derived from natural and synthetic polymers, including gelatin, hyaluronic acid, chondroitin sulfate, dextran, alginate, chitosan, heparin, and poly(ethylene glycol). A range of complex and heterogeneous structures are printed, including soft hydrogel constructs supporting the 3D culture of astrocytes. This highly generalizable methodology expands the palette of available bioinks, allowing the biofabrication of constructs optimized to meet the biological requirements of cell culture and tissue engineering.

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