Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis

Nano Letters ◽  
2022 ◽  
Author(s):  
Zhiyang Xu ◽  
Qihao Hong ◽  
Kun Ge ◽  
Xiaoyu Shi ◽  
Xiaolei Wang ◽  
...  
Keyword(s):  
Living Cells ◽  
Label Free ◽  
Random Lasing ◽  
Free Living

scholarly journals Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Masahiro Fukuta ◽  
Satoshi Kanamori ◽  
Taichi Furukawa ◽  
Yasunori Nawa ◽  
Wataru Inami ◽  
...  
Keyword(s):  
Electron Beam ◽  
Optical Microscope ◽  
Living Cells ◽  
Label Free ◽  
Free Living ◽  
Electron Beam Excitation ◽  
Nano Imaging ◽  
Beam Excitation

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

scholarly journals Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

10.3791/58486 ◽  
2018 ◽  
Author(s):  
André Gemeinhardt ◽  
Matthew P. McDonald ◽  
Katharina König ◽  
Michael Aigner ◽  
Andreas Mackensen ◽  
...  
Keyword(s):  
Living Cells ◽  
Label Free

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

scholarly journals Methods and applications of label-free cell-based systems

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Joachim Wiest
Keyword(s):  
Environmental Monitoring ◽  
Morphological Changes ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Controlled System ◽  
Chip Technology ◽  
Cell Monitoring ◽  
A Cell ◽  
On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

scholarly journals Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.

2010 ◽  
Vol 56 (4) ◽  
pp. 331-338 ◽  
Author(s):  
Valeria P. Conforte ◽  
Mariela Echeverria ◽  
Cintia Sánchez ◽  
Rodolfo A. Ugalde ◽  
Ana B. Menéndez ◽  
...  
Keyword(s):  
Acc Deaminase ◽  
Living Cells ◽  
Free Living ◽  
Mesorhizobium Loti

The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

2004 ◽  
Vol 25 (21-22) ◽  
pp. 3740-3745 ◽  
Author(s):  
Jurjen Emmelkamp ◽  
Floor Wolbers ◽  
Helene Andersson ◽  
Ralph S. DaCosta ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Microfluidic Systems ◽  
Single Living Cells ◽  
Single Living

A label-free multifunctional nanosensor based on N-doped carbon nanodots for vitamin B12 and Co2+ detection, and bioimaging in living cells and zebrafish

2020 ◽  
Vol 8 (23) ◽  
pp. 5089-5095
Author(s):  
Fangfang Du ◽  
Zhe Cheng ◽  
Marius Kremer ◽  
Yang Liu ◽  
Xiaodong Wang ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
A549 Cell ◽  
Living Cells ◽  
Carbon Nanodots ◽  
Label Free ◽  
Synthetic Procedure ◽  
Fluorescence Nanoprobe ◽  
Co2 Detection

Illustration of the synthetic procedure of N-CNDs and N-CDs-based bifunctional fluorescence nanoprobe for the detection of VB12 and Co2+ and bioimaging of A549 cell and zebrafish.

scholarly journals Acetoacetyl Coenzyme A Reductase and Polyhydroxybutyrate Synthesis in Rhizobium(Cicer) sp. Strain CC 1192

1998 ◽  
Vol 64 (8) ◽  
pp. 2859-2863 ◽  
Author(s):  
Shahid N. Chohan ◽  
Les Copeland
Keyword(s):  
Coenzyme A ◽  
Citrate Synthase ◽  
Living Cells ◽  
High Ratio ◽  
Free Living ◽  
Insoluble Material ◽  
Living State ◽  
Biochemical Level ◽  
Coa Reductase ◽  
Acetoacetyl Coa Reductase

ABSTRACT Biochemical controls that regulate the biosynthesis of poly-3-hydroxybutyrate (PHB) were investigated in Rhizobium(Cicer) sp. strain CC 1192. This species is of interest for studying PHB synthesis because the polymer accumulates to a large extent in free-living cells but not in bacteroids during nitrogen-fixing symbiosis with chickpea (Cicer arietinumL.) plants. Evidence is presented that indicates that CC 1192 cells retain the enzymic capacity to synthesize PHB when they differentiate from the free-living state to the bacteroid state. This evidence includes the incorporation by CC 1192 bacteroids of radiolabel from [14C]malate into 3-hydroxybutyrate which was derived by chemically degrading insoluble material from bacteroid pellets. Furthermore, the presence of an NADPH-dependent acetoacetyl coenzyme A (CoA) reductase, which was specific forR-(−)-3-hydroxybutyryl-CoA and NADP+ in the oxidative direction, was demonstrated in extracts from free-living and bacteroid cells of CC 1192. Activity of this enzyme in the reductive direction appeared to be regulated at the biochemical level mainly by the availability of substrates. The CC 1192 cells also contained an NADH-specific acetoacetyl-CoA reductase which oxidizedS-(+)-3-hydroxybutyryl-CoA. A membrane preparation from CC 1192 bacteroids readily oxidized NADH but not NADPH, which is suggested to be a major source of reductant for nitrogenase. Thus, a high ratio of NADPH to NADP+, which could enhance delivery of reductant to nitrogenase, could also favor the reduction of acetoacetyl-CoA for PHB synthesis. This would mean that fine controls that regulate the partitioning of acetyl-CoA between citrate synthase and 3-ketothiolase are important in determining whether PHB accumulates.

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