Abstract
Rapid identification of different amino acid responses is of great importance in the field of biomedicine. Through the combination of the drug intermediate pyrimidine with the fluorophore BODIPY, a new type of high-efficiency fluorescent probe for rapid identification of different amino acids was prepared. In terms of photophysical properties, the UV absorption and fluorescence emission of the mercapto groups were observed under the conditions of different hydroperoxide equivalents and different solvents, and the maximum values were in the range of 534–538 nm and 554–560 nm. When different amino acids were added, the UV absorption and fluorescence emission ranges were 538 ~ 540 nm and 560 ~ 564 nm. Finally, the identification sensitivity of amino acids with different equivalents under different oxidant conditions was quickly detected by fluorescence cuvette. Among them, the identification response to tyrosine was the most obvious.