We present an experimental study on the electrofluidic transistor in this paper. A novel and easy way to integrate the transistor into a microchannel is developed. The performances of the insulating layer, especially the leakage current under gate voltage, are carefully characterized. The change of surface charge on silica surface by gate polarization is measured, however, by measuring the streaming current, the gating effect on zeta potential has not been observed. This result should imply new assumption in the understanding of the charge regulation in the electrical double layer under gate polarization.