Integrating Thermal Sensors in a Microplate Format: Simultaneous Real-Time Quantification of Cell Number and Metabolic Activity

2022 ◽  
Author(s):  
Gilles Oudebrouckx ◽  
Juul Goossens ◽  
Seppe Bormans ◽  
Thijs Vandenryt ◽  
Patrick Wagner ◽  
...  
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Cell Number ◽  
Thermal Sensors

Species-specific real-time PCR cell number quantification of the bloom-forming cyanobacterium Planktothrix agardhii

2012 ◽  
Vol 194 (9) ◽  
pp. 749-757 ◽  
Author(s):  
Catarina Churro ◽  
Paulo Pereira ◽  
Vitor Vasconcelos ◽  
Elisabete Valério
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Cell Number ◽  
Planktothrix Agardhii ◽  
Species Specific

197 EFFECTS OF HYALURONAN ON OXYGEN CONSUMPTION AND ATP CONTENT IN PIG BLASTOCYSTS PRODUCED IN VITRO

2007 ◽  
Vol 19 (1) ◽  
pp. 215
Author(s):  
K. Yoshioka ◽  
M. Yokoo ◽  
T. Ozawa ◽  
C. Suzuki ◽  
H. Abe ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Metabolic Activity ◽  
Formation Rate ◽  
Defined Medium ◽  
Cell Number ◽  
Total Cell ◽  
Chemically Defined Medium ◽  
Atp Content ◽  
Cell Numbers

Hyaluronan (HA), a glycosaminoglycan present in follicular and oviductal fluids, has been related to sperm capacitation, fertilization, and embryo development. We have found that exogenous HA improves cell proliferation of porcine embryos cultured in a chemically defined medium (Yoshioka et al. 2004 Reprod. Fertil. Dev. 16, 264–265). Moreover, mitochondrial maturation was clearly more advanced in blastocysts cultured with HA compared to those cultured without HA, as seen by transmission electron microscopy. In the present study, the effects of HA on oxygen consumption and ATP content of blastocysts, produced in a defined system which reflects metabolic activity, were investigated. Porcine immature oocytes were matured for 44 h in porcine oocyte medium (POM) and subsequently fertilized with frozen–thawed ejaculated semen in porcine gamete medium supplemented with theophylline, adenosine, and cysteine (PGMtac4). Both POM and PGMtac4 were chemically defined media modified from porcine zygote medium (PZM)-5. After IVF, presumptive zygotes were cultured in PZM-5 containing HA (from the microorganism, Nacalai tesque, Kyoto, Japan) at concentrations of 0 [control], 10 [HA10], or 100 [HA100] �g mL-1 until 5 days after IVF. Blastocyst formation rate and total cell numbers/blastocyst at Day 5 were assessed. In addition, oxygen consumption and ATP content of single Day 5 blastocysts were measured. Blastocyst oxygen consumption was quantified using scanning electrochemical microscopy (HV-403; Research Institute for the Functional Peptides, Yamagata, Japan), and embryonic ATP content was determined using a commercial assay based on the luciferin-luciferase reaction (ATPlite; PerkinElmer, Groningen, The Netherlands). Data were statistically analyzed by ANOVA and Fisher's PLSD test. While the percentage of embryos that developed to the blastocyst stage [30.5% (63/206) to 31.7% (65/206)] did not differ among treatments, blastocyst cell number in the HA100 group [57.9 cells (n = 64)] was greater (P < 0.05) compared to those in the control [48.6 cells (n = 63)] or HA10 [50.0 cells (n = 65)] groups. Blastocyst oxygen consumption rate in the HA100 group [0.629 � 10-14 mol s-1 (n = 15)] was significantly higher than in the control [0.500 � 1-14 mol s-1 (n = 16)] or HA10 [0.464 � 10-14 mol s-1 (n = 14)] groups. ATP content/blastocyst did not differ among treatments [control: 0.645 pmol (n = 38), HA10: 0.727 pmol (n = 42), and HA100: 0.704 pmol (n = 43)]. It is concluded that HA affects the metabolic activity of pig blastocysts developed in a chemically defined medium, enhancing oxygen consumption and their total cell numbers, thus improving the quality of IVP blastocysts.

Retrofit Device for Real-Time Evaluation of Metabolic Activity in Herpes Virus Infected Cell Cultures

2014 ◽  
Vol 48 (4) ◽  
pp. 178-181 ◽  
Author(s):  
A. P. Vladimirov ◽  
A. S. Malygin ◽  
Yu. A. Mikhailova ◽  
A. A. Bakharev ◽  
A. P. Poryvaeva
Keyword(s):  
Infected Cell ◽  
Real Time ◽  
Cell Cultures ◽  
Metabolic Activity ◽  
Herpes Virus ◽  
Virus Infected Cell ◽  
Herpès Virus ◽  
Time Evaluation

scholarly journals Toggling Geobacter sulfurreducens metabolic state reveals hidden behaviour and expanded applicability to sustainable energy applications

2019 ◽  
Author(s):  
Mir Pouyan Zarabadi ◽  
Steve J. Charette ◽  
Jesse Greener
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Energy Production ◽  
Geobacter Sulfurreducens ◽  
Metabolic State ◽  
Flow Reactors ◽  
Energy Applications ◽  
Sustainable Technologies ◽  
Low Concentrations ◽  
Metabolic States

AbstractElectroactive biofilms are under intense scrutiny due to their potential to enable new sustainable technologies for energy production and bioremediation. However, severely reduced metabolic activity at low concentrations is a barrier to their implementation. A microfluidic approach was used for real-time respiration experiments on a Geobacter sulfurreducens biofilm to overcome these constraints. Precise changes to solution conditions enabled rapid and reversible switching between biofilm metabolic states, leading to the following discoveries. (i) Flow reactors can maintain biofilm activity at concentrations as low as 15 µM; (ii) a “pseudo-active” metabolic state separates active and inactive states; and (iii) acetate conversion can be as high as 90 percent for active biofilms at the pseudo-activity threshold.

scholarly journals Poloxamer 188 Exerts Direct Protective Effects on Mouse Brain Microvascular Endothelial Cells in an In Vitro Traumatic Brain Injury Model

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1043
Author(s):  
Felicia P. Lotze ◽  
Matthias L. Riess
Keyword(s):  
Traumatic Brain Injury ◽  
Endothelial Cells ◽  
Metabolic Activity ◽  
Membrane Damage ◽  
Cell Number ◽  
Microvascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Brain Microvascular Endothelial Cells ◽  
Compression Type

Traumatic Brain Injury (TBI), the main contributor to morbidity and mortality worldwide, can disrupt the cell membrane integrity of the vascular endothelial system, endangering blood–brain barrier function and threatening cellular subsistence. Protection of the vascular endothelial system might enhance clinical outcomes after TBI. Poloxamer 188 (P188) has been shown to improve neuronal function after ischemia/reperfusion (I/R) injury as well as after TBI. We aimed to establish an in vitro compression-type TBI model, comparing mild-to-moderate and severe injury, to observe the direct effects of P188 on Mouse Brain Microvascular Endothelial Cells (MBEC). Confluent MBEC were exposed to normoxic or hypoxic conditions for either 5 or 15 h (hours). 1 h compression was added, and P188 was administered during 2 h reoxygenation. A direct effect of P188 on MBEC was tested by assessing cell number/viability, cytotoxicity/membrane damage, metabolic activity, and total nitric oxide production (tNOp). While P188 enhanced cell number/viability, metabolic activity, and tNOp, an increase in cytotoxicity/membrane damage after mild-to-moderate injury was prevented. In severely injured MBEC, P188 improved metabolic activity only. P188, present during reoxygenation, influenced MBEC function directly in simulated I/R and compression-type TBI.

scholarly journals Real-time monitoring of cellular metabolic activity: intracellular oxygen

2016 ◽  
Vol 13 (10) ◽  
pp. i-ii
Author(s):  
Andrea Krumm ◽  
Conn Carey
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Real Time Monitoring ◽  
Cellular Metabolic Activity

scholarly journals Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyle B. Klopper ◽  
Riaan N. de Witt ◽  
Elanna Bester ◽  
Leon M. T. Dicks ◽  
Gideon M. Wolfaardt
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Continuous Flow ◽  
Control Strategies ◽  
Mass Function ◽  
Flow Conditions ◽  
Carbon Dioxide Evolution ◽  
Activity Measurements ◽  
The Internet Of Things

Abstract The tools used to study biofilms generally involve either destructive, end-point analyses or periodic measurements. The advent of the internet of things (IoT) era allows circumvention of these limitations. Here we introduce and detail the development of the BioSpec; a modular, nondestructive, real-time monitoring system, which accurately and reliably track changes in biofilm biomass over time. The performance of the system was validated using a commercial spectrophotometer and produced comparable results for variations in planktonic and sessile biomass. BioSpec was combined with the previously developed carbon dioxide evolution measurement system (CEMS) to allow simultaneous measurement of biofilm biomass and metabolic activity and revealed a differential response of these interrelated parameters to changing environmental conditions. The application of this system can facilitate a greater understanding of biofilm mass–function relationships and aid in the development of biofilm control strategies.

scholarly journals Real-time monitoring of bacterial biofilms metabolic activity by a redox-reactive nanosensors array

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Ella Yeor-Davidi ◽  
Marina Zverzhinetsky ◽  
Vadim Krivitsky ◽  
Fernando Patolsky
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Bacterial Biofilms ◽  
Real Time Monitoring
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