Overcoming Planktonic and Intracellular Staphylococcus aureus-Associated Infection with a Cell-Penetrating Peptide-Conjugated Antimicrobial Peptide

2020 ◽  
Vol 6 (12) ◽  
pp. 3147-3162
Author(s):  
Shicheng Huo ◽  
Chi Chen ◽  
Zhuocheng Lyu ◽  
Shutao Zhang ◽  
You Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Human antimicrobial peptide histatin 5 is a cell‐ penetrating peptide targeting mitochondrial ATP synthesis in Leishmania

2008 ◽  
Vol 22 (6) ◽  
pp. 1817-1828 ◽  
Author(s):  
Juan Román Luque‐Ortega ◽  
Wim Hof ◽  
Enno C. I. Veerman ◽  
José M. Saugar ◽  
Luis Rivas
Keyword(s):  
Antimicrobial Peptide ◽  
Atp Synthesis ◽  
Cell Penetrating Peptide ◽  
Histatin 5 ◽  
Cell Penetrating ◽  
A Cell ◽  
Peptide Targeting

scholarly journals A Phage Lysin Fused to a Cell-Penetrating Peptide Kills Intracellular Methicillin-Resistant Staphylococcus aureus in Keratinocytes and Has Potential as a Treatment for Skin Infections in Mice

2018 ◽  
Vol 84 (12) ◽  
Author(s):  
ZhaoFei Wang ◽  
LiCheng Kong ◽  
Yang Liu ◽  
Qiang Fu ◽  
ZeLin Cui ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistant Bacteria ◽  
Cell Penetrating Peptide ◽  
Skin Infections ◽  
Promising Alternative ◽  
Methicillin Resistant ◽  
Content Type ◽  
Skin Structure ◽  
Cell Penetrating ◽  
A Cell

ABSTRACT Staphylococcus aureus is the main pathogen that causes skin and skin structure infections and is able to survive and persist in keratinocytes of the epidermis. Since the evolution of multidrug-resistant bacteria, the use of phages and their lysins has presented a promising alternative approach to treatment. In this study, a cell wall hydrolase (also called lysin) derived from Staphylococcus phage JD007 (JDlys) was identified. JDlys showed strong lytic activity against methicillin-resistant Staphylococcus aureus (MRSA) strains from different sources and of different multilocus sequence typing (MLST) types. Furthermore, a fusion protein consisting of a cell-penetrating peptide derived from the trans -activating transcription (Tat) factor fused to JDlys (CPP Tat -JDlys) was used to kill MRSA bacteria causing intracellular infections. CPP Tat -JDlys, in which the fusion of CPP Tat to JDlys had almost no effect on the bacteriolytic activity of JDlys, was able to effectively eliminate intracellular MRSA bacteria and alleviate the inflammatory response and cell damage caused by MRSA. Specifically, CPP Tat -JDlys was able to combat MRSA-induced murine skin infections and, consequently, expedite the healing of cutaneous abscesses. These data suggest that the novel antimicrobial CPP-JDlys may be a worthwhile candidate as a treatment for skin and skin structure infections caused by MRSA. IMPORTANCE S. aureus is the main cause of skin and skin structure infections due to its ability to invade and survive in the epithelial barrier. Due to the overuse of antibiotics in humans and animals, S. aureus has shown a high capacity for acquiring and accumulating mechanisms of resistance to antibiotics. Moreover, most antibiotics are usually limited in their ability to overcome the intracellular persistence of bacteria causing skin and skin structure infections. So, it is critical to seek a novel antimicrobial agent to eradicate intracellular S. aureus . In this study, a cell-penetrating peptide fused to lysin (CPP-JDlys) was engineered. Our results show that CPP-JDlys can enter keratinocytes and effectively eliminate intracellular MRSA. Meanwhile, experiments with mice revealed that CPP-JDlys efficiently inhibits the proliferation of MRSA in murine skin and thus shortens the course of wound healing. Our results indicate that the CPP-fused lysin has potential for use for the treatment of skin infections caused by MRSA.

scholarly journals A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Makoto Oba ◽  
Takuma Kato ◽  
Kaori Furukawa ◽  
Masakazu Tanaka
Keyword(s):  
Gene Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Amine Structure ◽  
A Cell

Pharmacokinetics, Biodistribution, Stability and Toxicity of a Cell-Penetrating Peptide−Morpholino Oligomer Conjugate

2007 ◽  
Vol 18 (4) ◽  
pp. 1325-1331 ◽  
Author(s):  
Adams Amantana ◽  
Hong M. Moulton ◽  
Melissa L. Cate ◽  
Muralimohan T. Reddy ◽  
Tom Whitehead ◽  
...  
Keyword(s):  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

Matrix metalloproteinase-1 induces cleavage of exogenous alphab-crystallin transduced by a cell-penetrating peptide

2011 ◽  
Vol 112 (9) ◽  
pp. 2454-2462
Author(s):  
Seung Won Yang ◽  
Seung-Min Lee ◽  
Eun Young Choi ◽  
Kyung Hye Lee ◽  
Soo Hyuk Kim ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Cell Penetrating Peptide ◽  
Matrix Metalloproteinase 1 ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide

Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4082-4087 ◽  
Author(s):  
Likun Fei ◽  
Li-Peng Yap ◽  
Peter S. Conti ◽  
Wei-Chiang Shen ◽  
Jennica L. Zaro
Keyword(s):  
Tumor Targeting ◽  
Ph Sensitive ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Molecular Targeting of Cancer-Associated PCNA Interactions in Pancreatic Ductal Adenocarcinoma Using a Cell-Penetrating Peptide

2020 ◽  
Vol 17 ◽  
pp. 250-256
Author(s):  
Shanna J. Smith ◽  
Caroline M. Li ◽  
Robert G. Lingeman ◽  
Robert J. Hickey ◽  
Yilun Liu ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Molecular Targeting ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Construction, Expression, and Purification of p28 as a Cell-Penetrating Peptide ‎with Anticancer Effects on Burkitt’s Lymphoma Cell Line

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
Haniyeh Abuei ◽  
Abbas Behzad Behbahani ◽  
Gholamreza Rafiei Dehbidi ◽  
Mohammad Pirouzfar ◽  
Farahnaz Zare ◽  
...  
Keyword(s):  
Cell Line ◽  
Burkitt’S Lymphoma ◽  
Lymphoma Cell ◽  
Burkitt's Lymphoma ◽  
Cell Penetrating Peptide ◽  
Lymphoma Cell Line ◽  
Expression And Purification ◽  
Anticancer Effects ◽  
Cell Penetrating ◽  
A Cell
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