scholarly journals A Yeast-Based Biosensor for Screening of Short- and Medium-Chain Fatty Acid Production

2018 ◽  
Vol 7 (11) ◽  
pp. 2640-2646 ◽  
Author(s):  
Leonie Baumann ◽  
Arun S. Rajkumar ◽  
John P. Morrissey ◽  
Eckhard Boles ◽  
Mislav Oreb
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Chain Fatty Acid ◽  
Medium Chain Fatty Acid ◽  
Fatty Acid Production ◽  
Medium Chain

scholarly journals Matching Protein Interfaces for Improved Medium-Chain Fatty Acid Production

2018 ◽  
Vol 7 (5) ◽  
pp. 1179-1187 ◽  
Author(s):  
Stephen Sarria ◽  
Thomas G. Bartholow ◽  
Adam Verga ◽  
Michael D. Burkart ◽  
Pamela Peralta-Yahya
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Chain Fatty Acid ◽  
Medium Chain Fatty Acid ◽  
Fatty Acid Production ◽  
Medium Chain ◽  
Protein Interfaces

scholarly journals Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Matthew J. Scarborough ◽  
Griffin Lynch ◽  
Mitch Dickson ◽  
Mick McGee ◽  
Timothy J. Donohue ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Economic Value ◽  
Chain Fatty Acid ◽  
Medium Chain Fatty Acid ◽  
Fatty Acid Production ◽  
Medium Chain

Improving medium chain fatty acid production in Escherichia coli by multiple transporter engineering

2019 ◽  
Vol 272 ◽  
pp. 628-634 ◽  
Author(s):  
Junjun Wu ◽  
Zhe Wang ◽  
Xia Zhang ◽  
Peng Zhou ◽  
Xiudong Xia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Acid Production ◽  
Chain Fatty Acid ◽  
Medium Chain Fatty Acid ◽  
Fatty Acid Production ◽  
Medium Chain

scholarly journals Transcriptomic response of Saccharomyces cerevisiae to octanoic acid production

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Leonie Baumann ◽  
Tyler Doughty ◽  
Verena Siewers ◽  
Jens Nielsen ◽  
Eckhard Boles ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Acid Production ◽  
Octanoic Acid ◽  
Chain Fatty Acid ◽  
Medium Chain Fatty Acid ◽  
Cell Physiology ◽  
Transcriptomic Response ◽  
Promising Alternative ◽  
Medium Chain

ABSTRACT The medium-chain fatty acid octanoic acid is an important platform compound widely used in industry. The microbial production from sugars in Saccharomyces cerevisiae is a promising alternative to current non-sustainable production methods, however, titers need to be further increased. To achieve this, it is essential to have in-depth knowledge about the cell physiology during octanoic acid production. To this end, we collected the first RNA-Seq data of an octanoic acid producer strain at three time points during fermentation. The strain produced higher levels of octanoic acid and increased levels of fatty acids of other chain lengths (C6–C18) but showed decreased growth compared to the reference. Furthermore, we show that the here analyzed transcriptomic response to internally produced octanoic acid is notably distinct from a wild type's response to externally supplied octanoic acid as reported in previous publications. By comparing the transcriptomic response of different sampling times, we identified several genes that we subsequently overexpressed and knocked out, respectively. Hereby we identified RPL40B, to date unknown to play a role in fatty acid biosynthesis or medium-chain fatty acid tolerance. Overexpression of RPL40B led to an increase in octanoic acid titers by 40%.

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