Caged Chemotactic Peptides

2000 ◽  
Vol 11 (5) ◽  
pp. 679-681 ◽  
Author(s):  
Michael C. Pirrung ◽  
Sandra J. Drabik ◽  
Jasimuddin Ahamed ◽  
Hydar Ali
Keyword(s):  
Chemotactic Peptides

scholarly journals Identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages

1991 ◽  
Vol 266 (13) ◽  
pp. 8455-8463
Author(s):  
R.B. Goodman ◽  
J.W. Forstrom ◽  
S.G. Osborn ◽  
E.Y. Chi ◽  
T.R. Martin
Keyword(s):  
Alveolar Macrophages ◽  
Chemotactic Peptides

scholarly journals Role of peptide backbone conformation on biological activity of chemotactic peptides.

1991 ◽  
Vol 266 (28) ◽  
pp. 18460-18468
Author(s):  
A.R. Dentino ◽  
P.A. Raj ◽  
K.K. Bhandary ◽  
M.E. Wilson ◽  
M.J. Levine
Keyword(s):  
Biological Activity ◽  
Peptide Backbone ◽  
Backbone Conformation ◽  
Chemotactic Peptides ◽  
Peptide Backbone Conformation

Leukosialin (CD43, sialophorin) redistribution in uropods of polarized neutrophils is induced by CD43 cross-linking by antibodies, by colchicine or by chemotactic peptides

1997 ◽  
Vol 110 (13) ◽  
pp. 1465-1475
Author(s):  
S. Seveau ◽  
S. Lopez ◽  
P. Lesavre ◽  
J. Guichard ◽  
E.M. Cramer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Motility ◽  
Small Proportion ◽  
Cytochalasin B ◽  
Cell Polarization ◽  
Cross Linking ◽  
Chemotactic Peptides ◽  
Butanedione Monoxime ◽  
Major Surface ◽  
Triton X

We investigated a possible association of leukosialin (CD43), the major surface sialoglycoprotein of leukocytes, with neutrophil cytoskeleton. We first analysed the solubility of CD43 in Triton X-100 and observed that CD43 of resting neutrophils was mostly soluble. The small proportion of CD43 molecules, which ‘spontaneously’ precipitated in Triton, appeared associated with F-actin, as demonstrated by the fact that this insolubility did not occur when cells were incubated with cytochalasin B or when F-actin was depolymerized with DNase I in the Triton precipitate. Cell stimulation with anti-CD43 mAb (MEM59) enhanced this CD43-cytoskeleton association. By immunofluorescence as well as by electron microscopy, we observed a redistribution of CD43 on the neutrophil membrane, initially in patches followed by caps, during anti-CD43 cross-linking at 37 degrees C. This capping did not occur at 4 degrees C and was inhibited by cytochalasin B and by a myosin disrupting drug butanedione monoxime, thus providing evidence that the actomyosin contracile sytem is involved in the capping and further suggesting an association of CD43 with the cytoskeleton. Some of the capped cells exhibited a front-tail polarization with CD43 caps located in the uropod at the rear of the cell. Surprisingly, colchicine and the chemotactic factor fNLPNTL which induce neutrophil polarization associated with cell motility, also resulted in a clustering of CD43 in the uropod, independently of a cross-linking of the molecule by mAbs. An intracellular redistribution of F-actin, mainly at the leading front and of myosin in the tail, was observed during CD43 clustering induced by colchicine and in cells polarized by anti-CD43 mAbs cross-linking. We conclude that neutrophil CD43 interacts with the cytoskeleton, either directly or indirectly, to redistribute in the cell uropod under antibodies stimulation or during cell polarization by colchicine, thus highly suggesting that CD43 may be involved in cell polarization.

The protein kinase C inhibitor H-7 activates human neutrophils: effect on shape, actin polymerization, fluid pinocytosis and locomotion

1990 ◽  
Vol 96 (1) ◽  
pp. 99-106
Author(s):  
H.U. Keller ◽  
V. Niggli ◽  
A. Zimmermann ◽  
R. Portmann
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Actin Polymerization ◽  
Kinase Inhibitor ◽  
Human Neutrophils ◽  
Kinase C ◽  
Chemotactic Peptides ◽  
Protein Kinase C Inhibitor ◽  
Shape Changes ◽  
Stimulation Of

The present study demonstrates new properties of H-7. The protein kinase inhibitor H-7 is a potent activator of several neutrophil functions. Stimulation of initially spherical nonmotile neutrophils elicits vigorous shape changes within a few seconds, increases in cytoskeletal actin, altered F-actin distribution, increased adhesiveness and a relatively small increase in pinocytic activity. H-7 has also chemokinetic activities. Depending on the experimental condition, H-7 may elicit or inhibit neutrophil locomotion. It failed to induce chemotaxis. Thus, the response pattern elicited by H-7 is different from that of other leukocyte activators such as chemotactic peptides, PMA or diacylglycerols. The finding that H-7 can elicit shape changes, actin polymerization and pinocytosis suggests that these events can occur without activation of protein kinase C (PKC). PMA-induced shape changes and stimulation of pinocytosis were not inhibited by H-7.

scholarly journals H-2M3a violates the paradigm for major histocompatibility complex class I peptide binding.

1995 ◽  
Vol 181 (5) ◽  
pp. 1817-1825 ◽  
Author(s):  
J M Vyas ◽  
J R Rodgers ◽  
R R Rich
Keyword(s):  
Amino Acids ◽  
Mhc Class I ◽  
Temperature Shift ◽  
Class I ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Compensatory Adaptation ◽  
Chemotactic Peptides ◽  
The Stability ◽  
Formyl Peptides

The major histocompatibility (MHC) class I-b molecule H-2M3a binds and presents N-formylated peptides to cytotoxic T lymphocytes. This requirement potentially places severe constraints on the number of peptides that M3a can present to the immune system. Consistent with this idea, the M3a-Ld MHC class I chimera is expressed at very low levels on the cell surface, but can be induced significantly by the addition of specific peptides at 27 degrees C. Using this assay, we show that M3a binds many very short N-formyl peptides, including N-formyl chemotactic peptides and canonical octapeptides. This observation is in sharp contrast to the paradigmatic size range of peptides of 8-10 amino acids binding to most class I-a molecules and the class I-b molecule Qa-2. Stabilization by fMLF-benzyl amide could be detected at peptide concentrations as low as 100 nM. While N-formyl peptides as short as two amino acids in length stabilized expression of M3a-Ld, increasing the length of these peptides added to the stability of peptide-MHC complexes as determined by 27-37 degrees C temperature shift experiments. We propose that relaxation of the length rule may represent a compensatory adaptation to maximize the number of peptides that can be presented by H-2M3a.

Chemotactic peptides

1988 ◽  
Vol 33 (S3) ◽  
pp. 50S-57S ◽  
Author(s):  
Cynthia C. Nast ◽  
Louise E. LeDuc
Keyword(s):  
Chemotactic Peptides

[13] Synthesis of chemotactic peptides

1988 ◽  
pp. 132-139 ◽  
Author(s):  
Natesa Muthukumaraswamy ◽  
Richard J. Freer
Keyword(s):  
Chemotactic Peptides

Syntheses and chemotactic activities of [2,3-methanophenylalanine3]chemotactic peptides

Peptides ◽  
1992 ◽  
pp. 423-424 ◽  
Author(s):  
Hiroaki Kodama ◽  
Masaya Miyazaki ◽  
Michio Kondo ◽  
Kazuyasu Sakaguchi ◽  
Charles H. Stammer ◽  
...  
Keyword(s):  
Chemotactic Peptides

scholarly journals Structure and Activity of Chemotactic Peptides from Wasp Venom and Frog Skin

1987 ◽  
Vol 43 ◽  
pp. 92
Author(s):  
Yoshio Tsukamoto ◽  
Chieko Mori ◽  
Sachiko Fukutani ◽  
Masakazu Mori ◽  
Tadashi Yasuhara ◽  
...  
Keyword(s):  
Frog Skin ◽  
Wasp Venom ◽  
Chemotactic Peptides ◽  
Structure And Activity
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