Two-dimensional NMR studies of staphylococcal nuclease: evidence for conformational heterogeneity from hydrogen-1, carbon-13, and nitrogen-15 spin system assignments of the aromatic amino acids in the nuclease H124L-thymidine 3',5'-bisphosphate-calcium(2+) ternary complex

We report for the very first time self-assembly of Cysteine and Methionine to discrenible strucutres under neutral condition. To get insights into the structure formation, thioflavin T and Congo red binding assays were done which revealed that aggregates may not have amyloid like characteristics. The nature of interactions which lead to such self-assemblies was purported by coincubating assemblies in urea and mercaptoethanol. Further interaction of aggregates with short amyloidogenic dipeptide diphenylalanine (FF) was assessed. While cysteine aggregates completely disrupted FF fibres, methionine albeit triggered fibrillation. The cytotoxicity assays of cysteine and methionine structures were performed on Human Neuroblastoma IMR-32 cells which suggested that aggregates are not cytotoxic in nature and thus, may not have amyloid like etiology. The results presented in the manuscript are striking, since to the best of our knowledge,this is the first report which demonstrates that even non-aromatic amino acids (cysteine and methionine) can undergo spontaneous self-assembly to form ordered aggregates.

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Faculty Opinions recommendation of Auto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5915956.6185054 ◽

2010 ◽

Author(s):

Sherry Mowbray ◽

Daniel Ericsson

Keyword(s):

Amino Acids ◽

Aromatic Amino Acids ◽

Induction Medium ◽

High Level

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New Methods for Analyzing Water Pollutants

Water Science & Technology ◽

10.2166/wst.1982.0087 ◽

1982 ◽

Vol 14 (4-5) ◽

pp. 59-71 ◽

Cited By ~ 6

Author(s):

L H Keith ◽

R C Hall ◽

R C Hanisch ◽

R G Landolt ◽

J E Henderson

Keyword(s):

Amino Acids ◽

Gas Chromatography ◽

Two Dimensional ◽

Gas Chromatograph ◽

New Class ◽

New Methods ◽

System A ◽

Drinking Waters ◽

Computer Controlled ◽

Nitrogen Containing Compounds

Two new methods have been developed to analyze for organic pollutants in water. The first, two-dimensional gas chromatography, using post detector peak recycling (PDPR), involves the use of a computer-controlled gas Chromatograph to selectively trap compounds of interest and rechromatograph them on a second column, recycling them through the same detector again. The second employs a new detector system, a thermally modulated electron capture detector (TMECD). Both methods were used to demonstrate their utility by applying them to the analysis of a new class of potentially ubiquitous anthropoaqueous pollutants in drinking waters- -haloacetonitriles. These newly identified compounds are produced from certain amino acids and other nitrogen-containing compounds reacting with chlorine during the disinfection stage of treatment.

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