3‘-5‘ Exonuclease of Klenow Fragment:  Role of Amino Acid Residues within the Single-Stranded DNA Binding Region in Exonucleolysis and Duplex DNA Melting†

Biochemistry ◽  
2002 ◽  
Vol 41 (12) ◽  
pp. 3943-3951 ◽  
Author(s):  
Wai-Chung Lam ◽  
Elizabeth H. Z. Thompson ◽  
Olga Potapova ◽  
Xiaojun Chen Sun ◽  
Catherine M. Joyce ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dna Binding ◽  
Dna Melting ◽  
Duplex Dna ◽  
Amino Acid Residues ◽  
Klenow Fragment ◽  
Binding Region ◽  
Single Stranded Dna

scholarly journals Essential Amino Acid Residues in the Single-stranded DNA-binding Protein of Bacteriophage T7

2002 ◽  
Vol 277 (52) ◽  
pp. 50643-50653 ◽  
Author(s):  
Lisa F. Rezende ◽  
Thomas Hollis ◽  
Tom Ellenberger ◽  
Charles C. Richardson
Keyword(s):  
Amino Acid ◽  
Dna Binding ◽  
Essential Amino Acid ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Bacteriophage T7 ◽  
Amino Acid Residues ◽  
Single Stranded Dna

Role of Specific Amino Acid Residues in T4 Endonuclease V That Alter Nontarget DNA Binding†

Biochemistry ◽  
1997 ◽  
Vol 36 (14) ◽  
pp. 4080-4088 ◽  
Author(s):  
Simon G. Nyaga ◽  
M. L. Dodson ◽  
R. Stephen Lloyd
Keyword(s):  
Amino Acid ◽  
Dna Binding ◽  
Amino Acid Residues ◽  
Specific Amino Acid ◽  
Endonuclease V

Role of Ionizing Amino Acid Residues in the Process of DNA Binding by Human AP Endonuclease 1 and in Its Catalysis

2019 ◽  
Vol 123 (45) ◽  
pp. 9546-9556 ◽  
Author(s):  
Irina V. Alekseeva ◽  
Artemiy S. Bakman ◽  
Yury N. Vorobjev ◽  
Olga S. Fedorova ◽  
Nikita A. Kuznetsov
Keyword(s):  
Amino Acid ◽  
Dna Binding ◽  
Amino Acid Residues ◽  
Ap Endonuclease ◽  
Ap Endonuclease 1

scholarly journals Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to Reduced Cell Complexity in Cultured Hippocampal Neurons—New Insights into the Role of the C-Terminal Region of Tpm3.1

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 715
Author(s):  
Tamara Tomanić ◽  
Claire Martin ◽  
Holly Stefen ◽  
Esmeralda Parić ◽  
Peter Gunning ◽  
...  
Keyword(s):  
Amino Acid ◽  
Hippocampal Neurons ◽  
Molecular Mechanisms ◽  
Growth Cones ◽  
Amino Acid Residues ◽  
Terminal Amino Acid ◽  
C Terminus ◽  
Primary Mouse ◽  
Terminal Amino

Tropomyosins (Tpms) have been described as master regulators of actin, with Tpm3 products shown to be involved in early developmental processes, and the Tpm3 isoform Tpm3.1 controlling changes in the size of neuronal growth cones and neurite growth. Here, we used primary mouse hippocampal neurons of C57/Bl6 wild type and Bl6Tpm3flox transgenic mice to carry out morphometric analyses in response to the absence of Tpm3 products, as well as to investigate the effect of C-terminal truncation on the ability of Tpm3.1 to modulate neuronal morphogenesis. We found that the knock-out of Tpm3 leads to decreased neurite length and complexity, and that the deletion of two amino acid residues at the C-terminus of Tpm3.1 leads to more detrimental changes in neurite morphology than the deletion of six amino acid residues. We also found that Tpm3.1 that lacks the 6 C-terminal amino acid residues does not associate with stress fibres, does not segregate to the tips of neurites, and does not impact the amount of the filamentous actin pool at the axonal growth cones, as opposed to Tpm3.1, which lacks the two C-terminal amino acid residues. Our study provides further insight into the role of both Tpm3 products and the C-terminus of Tpm3.1, and it forms the basis for future studies that aim to identify the molecular mechanisms underlying Tpm3.1 targeting to different subcellular compartments.

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