scholarly journals Structural Insights into the Pre-Amyloid Tetramer of β-2-Microglobulin from Covalent Labeling and Mass Spectrometry

Biochemistry ◽  
2011 ◽  
Vol 50 (31) ◽  
pp. 6711-6722 ◽  
Author(s):  
Vanessa Leah Mendoza ◽  
Mario A. Barón-Rodríguez ◽  
Cristian Blanco ◽  
Richard W. Vachet
Keyword(s):  
Mass Spectrometry ◽  
Covalent Labeling ◽  
Structural Insights

scholarly journals Structural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility–mass spectrometry

2019 ◽  
Vol 476 (21) ◽  
pp. 3125-3139 ◽  
Author(s):  
Daniel Shiu-Hin Chan ◽  
Jeannine Hess ◽  
Elen Shaw ◽  
Christina Spry ◽  
Robert Starley ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Structural Biology ◽  
Ion Mobility ◽  
Biosynthetic Pathway ◽  
Screening Tool ◽  
Native Mass Spectrometry ◽  
Ion Mobility Mass Spectrometry ◽  
E Coli ◽  
Structural Insights

Abstract CoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility–mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.

scholarly journals Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis

2021 ◽  
Vol 116 ◽  
Author(s):  
Jhenifer Yonara de Lima ◽  
Marlon Dias Mariano Santos ◽  
Mario Tyago Murakami ◽  
Paulo Costa Carvalho ◽  
Tatiana de Arruda Campos Brasil de Souza
Keyword(s):  
Mass Spectrometry ◽  
Glutamine Synthetase ◽  
Cross Linking ◽  
Leishmania Braziliensis ◽  
Structural Insights

scholarly journals Structural Insights into Fibrinogen Dynamics Using Amide Hydrogen/Deuterium Exchange Mass Spectrometry

Biochemistry ◽  
2013 ◽  
Vol 52 (32) ◽  
pp. 5491-5502 ◽  
Author(s):  
James J. Marsh ◽  
Henry S. Guan ◽  
Sheng Li ◽  
Peter G. Chiles ◽  
Danny Tran ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Amide Hydrogen ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Structural Insights

scholarly journals Structural insights into glutathione-protected gold Au10−12(SG)10−12 nanoclusters revealed by ion mobility mass spectrometry

2018 ◽  
Vol 72 (8) ◽  
Author(s):  
Clothilde Comby-Zerbino ◽  
Franck Bertorelle ◽  
Fabien Chirot ◽  
Philippe Dugourd ◽  
Rodolphe Antoine
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Structural Insights

scholarly journals Structural insights into the EthR–DNA interaction using native mass spectrometry

2017 ◽  
Vol 53 (25) ◽  
pp. 3527-3530 ◽  
Author(s):  
Daniel Shiu-Hin Chan ◽  
Wei-Guang Seetoh ◽  
Brendan N. McConnell ◽  
Dijana Matak-Vinković ◽  
Sherine E. Thomas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mycobacterium Tuberculosis ◽  
Dna Interaction ◽  
Native Mass Spectrometry ◽  
Operator Dna ◽  
Structural Insights

The interaction between Mycobacterium tuberculosis EthR and its operator DNA has been studied by native mass spectrometry, revealing an interesting stoichiometry.

Fragmentation of Transition Metal Carbonyl Cluster Anions: Structural Insights from Mass Spectrometry

2003 ◽  
Vol 9 (4) ◽  
pp. 944-950 ◽  
Author(s):  
Colin P. G. Butcher ◽  
Paul J. Dyson ◽  
Brian F. G. Johnson ◽  
Tetyana Khimyak ◽  
J. Scott McIndoe
Keyword(s):  
Mass Spectrometry ◽  
Transition Metal ◽  
Metal Carbonyl ◽  
Carbonyl Cluster ◽  
Cluster Anions ◽  
Metal Carbonyl Cluster ◽  
Structural Insights

Structural insights into calmodulin/Munc13 interaction

2014 ◽  
Vol 395 (7-8) ◽  
pp. 763-768 ◽  
Author(s):  
Sabine Herbst ◽  
Noa Lipstein ◽  
Olaf Jahn ◽  
Andrea Sinz
Keyword(s):  
Mass Spectrometry ◽  
Synaptic Plasticity ◽  
Binding Site ◽  
Cross Linking ◽  
Binding Motif ◽  
Binding Modes ◽  
Short Term ◽  
Common Domain ◽  
Structural Insights ◽  
Chemical Cross Linking

Abstract Munc13 proteins are essential presynaptic regulators that mediate synaptic vesicle priming and play a role in the regulation of neuronal short-term synaptic plasticity. All four Munc13 isoforms share a common domain structure, including a calmodulin (CaM) binding site in their otherwise divergent N-termini. Here, we summarize recent results on the investigation of the CaM/Munc13 interaction. By combining chemical cross-linking, photoaffinity labeling, and mass spectrometry, we showed that all neuronal Munc13 isoforms exhibit similar CaM binding modes. Moreover, we demonstrated that the 1-5-8-26 CaM binding motif discovered in Munc13-1 cannot be induced in the classical CaM target skMLCK, indicating unique features of the Munc13 CaM binding motif.

Sign in / Sign up

Export Citation Format

Share Document