Highly Protein-Resistant Coatings and Suspension Cell Culture Thereon from Amphiphilic Block Copolymers Prepared by RAFT Polymerization

2014 ◽  
Vol 15 (6) ◽  
pp. 1992-2003 ◽  
Author(s):  
Kazutoshi Haraguchi ◽  
Kazuomi Kubota ◽  
Tetsuo Takada ◽  
Saori Mahara
Keyword(s):  
Cell Culture ◽  
Block Copolymers ◽  
Raft Polymerization ◽  
Amphiphilic Block Copolymers ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Protein Resistant

Respiration activity of suspension cell culture of Polyscias filicifolia bailey, Stephania glabra (Roxb.) miers, and Dioscorea deltoidea wall

2011 ◽  
Vol 47 (1) ◽  
pp. 87-92 ◽  
Author(s):  
M. V. Titova ◽  
E. A. Berkovich ◽  
O. V. Reshetnyak ◽  
I. E. Kulichenko ◽  
A. V. Oreshnikov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Respiration Activity ◽  
Dioscorea Deltoidea

scholarly journals Growth of Respiratory Syncytial Virus in Suspension Cell Culture

1971 ◽  
Vol 22 (6) ◽  
pp. 1123-1125
Author(s):  
Alan V. Richman ◽  
Nicola M. Tauraso
Keyword(s):  
Cell Culture ◽  
Respiratory Syncytial Virus ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Syncytial Virus

One-step synthesis of poly(2-oxazoline)-based amphiphilic block copolymers using a dual initiator for RAFT polymerization and CROP

Polymer ◽  
2014 ◽  
Vol 55 (23) ◽  
pp. 5986-5990 ◽  
Author(s):  
Young Chang Yu ◽  
Hang Sung Cho ◽  
Woong-Ryeol Yu ◽  
Ji Ho Youk
Keyword(s):  
Block Copolymers ◽  
Raft Polymerization ◽  
Amphiphilic Block Copolymers ◽  
One Step

scholarly journals Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 671 ◽  
Author(s):  
Pucker ◽  
Rückert ◽  
Stracke ◽  
Viehöver ◽  
Kalinowski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Arabidopsis Thaliana ◽  
Large Scale ◽  
Suspension Cell ◽  
Reference Sequence ◽  
Model Organisms ◽  
Suspension Cell Culture ◽  
Single Nucleotide Variants ◽  
Single Nucleotide ◽  
Haploid Genome Size

Arabidopsis thaliana is one of the best studied plant model organisms. Besides cultivation in greenhouses, cells of this plant can also be propagated in suspension cell culture. At7 is one such cell line that was established about 25 years ago. Here, we report the sequencing and the analysis of the At7 genome. Large scale duplications and deletions compared to the Columbia-0 (Col-0) reference sequence were detected. The number of deletions exceeds the number of insertions, thus indicating that a haploid genome size reduction is ongoing. Patterns of small sequence variants differ from the ones observed between A. thaliana accessions, e.g., the number of single nucleotide variants matches the number of insertions/deletions. RNA-Seq analysis reveals that disrupted alleles are less frequent in the transcriptome than the native ones.

Synthesis of amphiphilic block copolymers based on poly(dimethylsiloxane) via fragmentation chain transfer (RAFT) polymerization

Polymer ◽  
2004 ◽  
Vol 45 (13) ◽  
pp. 4383-4389 ◽  
Author(s):  
Tommy S.C Pai ◽  
Christopher Barner-Kowollik ◽  
Thomas P Davis ◽  
Martina H Stenzel
Keyword(s):  
Block Copolymers ◽  
Chain Transfer ◽  
Raft Polymerization ◽  
Amphiphilic Block Copolymers

scholarly journals Photo-Responsive Polymersomes as Drug Delivery System for Potential Medical Applications

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5147
Author(s):  
Wanting Hou ◽  
Ruiqi Liu ◽  
Siwei Bi ◽  
Qian He ◽  
Haibo Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Block Copolymers ◽  
Drug Delivery System ◽  
Delivery System ◽  
Raft Polymerization ◽  
Amphiphilic Block Copolymers ◽  
Medical Applications ◽  
Drug Molecules ◽  
Reversible Addition ◽  
Raft Agent

Due to a strong retardation effect of o-nitrobenzyl ester on polymerization, it is still a great challenge to prepare amphiphilic block copolymers for polymersomes with a o-nitrobenzyl ester-based hydrophobic block. Herein, we present one such solution to prepare amphiphilic block copolymers with pure poly (o-nitrobenzyl acrylate) (PNBA) as the hydrophobic block and poly (N,N’-dimethylacrylamide) (PDMA) as the hydrophilic block using bulk reversible addition-fragmentation chain transfer (RAFT) polymerization of o-nitrobenzyl acrylate using a PDMA macro-RAFT agent. The developed amphiphilic block copolymers have a suitable hydrophobic/hydrophilic ratio and can self-assemble into photoresponsive polymersomes for co-loading hydrophobic and hydrophilic cargos into hydrophobic membranes and aqueous compartments of the polymersomes. The polymersomes demonstrate a clear photo-responsive characteristic. Exposure to light irradiation at 365 nm can trigger a photocleavage reaction of o-nitrobenzyl groups, which results in dissociation of the polymersomes with simultaneous co-release of hydrophilic and hydrophobic cargoes on demand. Therefore, these polymersomes have great potential as a smart drug delivery nanocarrier for controllable loading and releasing of hydrophilic and hydrophobic drug molecules. Moreover, taking advantage of the conditional releasing of hydrophilic and hydrophobic drugs, the drug delivery system has potential use in medical applications such as cancer therapy.

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