Production of HIV-1 gp120 in Packed-Bed Bioreactor Using the Vaccinia Virus/T7 Expression System

2000 ◽  
Vol 16 (5) ◽  
pp. 744-750 ◽  
Author(s):  
Y.-C. Hu ◽  
J. Kaufman ◽  
M.W. Cho ◽  
H. Golding ◽  
J. Shiloach
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Packed Bed ◽  
Packed Bed Bioreactor ◽  
T7 Expression System ◽  
Hiv 1

scholarly journals Viable double vaccinia virus recombinants with the non-inducible phage T7 expression system

1993 ◽  
Vol 74 (1) ◽  
pp. 47-53 ◽  
Author(s):  
M. V. Kriajevska ◽  
L. G. Zakharova ◽  
A. D. Altstein
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Phage T7 ◽  
T7 Expression System

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Three-dimensional CFD simulation of anaerobic reactions in a continuous packed-bed bioreactor

2021 ◽  
Author(s):  
Sasan Zarei ◽  
Seyyed Mohammad Mousavi ◽  
Teimour Amani ◽  
Mehrdad Khamforoush ◽  
Arezou Jafari
Keyword(s):  
Cfd Simulation ◽  
Three Dimensional ◽  
Packed Bed ◽  
Packed Bed Bioreactor

Toxicity and recalcitrant compound removal from bleaching pulp plant effluents by an integrated system: anaerobic packed-bed bioreactor and ozone

2010 ◽  
Vol 61 (1) ◽  
pp. 199-205 ◽  
Author(s):  
T. R. Chaparro ◽  
C. M. Botta ◽  
E. C. Pires
Keyword(s):  
Pulp Mill ◽  
Packed Bed ◽  
Ultraviolet Absorption ◽  
Integrated System ◽  
Packed Bed Bioreactor ◽  
Cellulose Pulp ◽  
Adsorbable Organic Halides ◽  
Organic Halides ◽  
Before And After ◽  
Absorption Measurements

Effluents originated in cellulose pulp manufacturing processes are usually toxic and recalcitrant, specially the bleaching effluents, which exhibit high contents of aromatic compounds (e.g. residual lignin derivates). Although biological processes are normally used, their efficiency for the removal of toxic lignin derivates is low. The toxicity and recalcitrance of a bleached Kraft pulp mill were assessed through bioassays and ultraviolet absorption measurements, i.e. acid soluble lignin (ASL), UV280, and specific ultraviolet absorption (SUVA), before and after treatment by an integrated system comprised of an anaerobic packed-bed bioreactor and oxidation step with ozone. Furthermore, adsorbable organic halides (AOX) were measured. The results demonstrated not only that the toxic recalcitrant compounds can be removed successfully using integrated system, but also the ultraviolet absorption measurements can be an interesting control-parameter in a wastewater treatment.

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