Thermodynamics of Metabolic Pathways for Penicillin Production: Analysis of Thermodynamic Feasibility and Free Energy Changes During Fed-Batch Cultivation

1997 ◽  
Vol 13 (2) ◽  
pp. 156-165 ◽  
Author(s):  
P.D.N. Pissarra ◽  
J. Nielsen
Keyword(s):  
Free Energy ◽  
Metabolic Pathways ◽  
Batch Cultivation ◽  
Penicillin Production ◽  
Fed Batch ◽  
Production Analysis ◽  
Thermodynamic Feasibility ◽  
Fed Batch Cultivation

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

A feedforward–feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase

2005 ◽  
Vol 29 (5) ◽  
pp. 1113-1120 ◽  
Author(s):  
M. Arndt ◽  
S. Kleist ◽  
G. Miksch ◽  
K. Friehs ◽  
E. Flaschel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kalman Filter ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Fed-Batch Cultivation of Saccharomyces cerevisiae in a Hyperbaric Bioreactor

2003 ◽  
Vol 19 (2) ◽  
pp. 665-671 ◽  
Author(s):  
I. Belo ◽  
R. Pinheiro ◽  
M. Mota
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

Fed-batch cultivation of Desmodesmus sp. in anaerobic digestion wastewater for improved nutrient removal and biodiesel production

2015 ◽  
Vol 184 ◽  
pp. 116-122 ◽  
Author(s):  
Fang Ji ◽  
Yuguang Zhou ◽  
Aiping Pang ◽  
Li Ning ◽  
Kibet Rodgers ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Nutrient Removal ◽  
Batch Cultivation ◽  
Biodiesel Production ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Xylitol production by Candida parapsilosis under fed-batch culture

2001 ◽  
Vol 44 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Sandra A. Furlan ◽  
Heizir F. de Castro
Keyword(s):  
Candida Parapsilosis ◽  
Batch Cultivation ◽  
Xylitol Production ◽  
Fed Batch ◽  
Substrate Consumption ◽  
Feed Medium ◽  
Fed Batch Culture ◽  
Rates Of Growth ◽  
Fed Batch Cultivation ◽  
Single Batch

Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.

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