Endogenous Ethanol Affects Biopolyester Molecular Weight in Recombinant Escherichia coli

2013 ◽  
Vol 8 (11) ◽  
pp. 2568-2576 ◽  
Author(s):  
Ayaka Hiroe ◽  
Manami Hyakutake ◽  
Nicholas M. Thomson ◽  
Easan Sivaniah ◽  
Takeharu Tsuge
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Endogenous Ethanol

Effective production and kinetic characterization of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

2005 ◽  
Vol 87 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Prihardi Kahar ◽  
Jumiarti Agus ◽  
Yoshihiro Kikkawa ◽  
Kazunori Taguchi ◽  
Yoshiharu Doi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Kinetic Characterization ◽  
Ultra High Molecular Weight ◽  
High Molecular Weight Poly

Molecular Weight Change of Polyhydroxyalkanoate (PHA) Caused by the PhaC Subunit of PHA Synthase from Bacillus cereus YB-4 in Recombinant Escherichia coli

2011 ◽  
Vol 12 (7) ◽  
pp. 2660-2666 ◽  
Author(s):  
Satoshi Tomizawa ◽  
Manami Hyakutake ◽  
Yuta Saito ◽  
Jumiarti Agus ◽  
Kouhei Mizuno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Bacillus Cereus ◽  
Weight Change ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase

Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide

2016 ◽  
Vol 100 (18) ◽  
pp. 7877-7885 ◽  
Author(s):  
Haichan Huang ◽  
Xiaobo Liu ◽  
Shencong Lv ◽  
Weihong Zhong ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Recombinant Escherichia Coli

Influence of pH on the Molecular Weight of Poly-3-hydroxybutyric Acid (P3HB) Produced by Recombinant Escherichia coli

2013 ◽  
Vol 170 (6) ◽  
pp. 1336-1347 ◽  
Author(s):  
Johanna Katherine Bocanegra ◽  
José Geraldo da Cruz Pradella ◽  
Luiziana Ferreira da Silva ◽  
Marilda Keico Taciro ◽  
Jose Gregório Cabrera Gomez
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Hydroxybutyric Acid ◽  
Influence Of Ph

Evaluation of Stemness Maintenance Properties of the Recombinant Human Laminin α2 LG1-3 Domains in Human Mesenchymal Stem Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 785-791
Author(s):  
Ji-Eun Kim ◽  
Hye-Jin Seo ◽  
SuJin Lee ◽  
Jun-Hyeog Jang
Keyword(s):  
Escherichia Coli ◽  
Stem Cells ◽  
Molecular Weight ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Human Mesenchymal Stem Cells ◽  
Adhesion Assay ◽  
Proliferation Assay ◽  
Undifferentiated State ◽  
Laminin Α2

Background: Laminin, a member of the Extracellular Matrix (ECM), is a glycoprotein that is used as a factor that affects cell adhesion, proliferation, survival, and differentiation. Of these, five globular domains (LG domains) of the alpha chain play an important role in influencing the cell by binding to the integrin. Objective: This study aimed to evaluate the ability of globular domains 1-3 of laminin alpha2 (rhLAMA2LG1-3) in maintaining the pluripotency of human Mesenchymal Stem Cells (hMSCs), which are widely used in regenerative medicine. Methods: hMSCs were grown in the medium supplemented with rhLAMA2LG1-3, then the effect of the protein on hMSCs were confirmed through cell adhesion assay, proliferation assay and RTPCR. Results: rhLAMA2LG1-3 expressed in Escherichia coli has a molecular weight of 70 kDa, at 1 µg/ml concentration of rhLAMA2LG1-3, the attachment and proliferation of hMSCs were approximately 3.18-fold and 1.67-fold, respectively, more efficient than those of untreated controls. In addition, the undifferentiated state and degree of stemness of hMSCs were measured, on the basis of CD90 and CD105 levels. In the rhLAMA2LG1-3-treated hMSCs, the expression levels of CD90 and CD105 increased by 2.83-fold and 1.62-fold, respectively, compared to those in untreated controls. Conclusion: rhLAMA2LG1-3 can be potentially used in stem cell therapy to improve the viability and maintain the undifferentiated state of hMSCs.

Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion

2000 ◽  
Vol 45 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Man Bock Gu ◽  
Jiho Min ◽  
Robert A LaRossa
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli
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