Molecular Weight Change of Polyhydroxyalkanoate (PHA) Caused by the PhaC Subunit of PHA Synthase from Bacillus cereus YB-4 in Recombinant Escherichia coli

2011 ◽  
Vol 12 (7) ◽  
pp. 2660-2666 ◽  
Author(s):  
Satoshi Tomizawa ◽  
Manami Hyakutake ◽  
Yuta Saito ◽  
Jumiarti Agus ◽  
Kouhei Mizuno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Bacillus Cereus ◽  
Weight Change ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase

Effective production and kinetic characterization of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

2005 ◽  
Vol 87 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Prihardi Kahar ◽  
Jumiarti Agus ◽  
Yoshihiro Kikkawa ◽  
Kazunori Taguchi ◽  
Yoshiharu Doi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Kinetic Characterization ◽  
Ultra High Molecular Weight ◽  
High Molecular Weight Poly

scholarly journals Rearrangement of Gene Order in thephaCABOperon Leads to Effective Production of Ultrahigh-Molecular-Weight Poly[(R)-3-Hydroxybutyrate] in Genetically Engineered Escherichia coli

2012 ◽  
Vol 78 (9) ◽  
pp. 3177-3184 ◽  
Author(s):  
Ayaka Hiroe ◽  
Kenji Tsuge ◽  
Christopher T. Nomura ◽  
Mitsuhiro Itaya ◽  
Takeharu Tsuge
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Gene Order ◽  
Ralstonia Eutropha ◽  
Genetically Engineered ◽  
Pha Synthase ◽  
Limiting Factor ◽  
Expression Plasmid ◽  
Content Type ◽  
Ultrahigh Molecular Weight

ABSTRACTUltrahigh-molecular-weight poly[(R)-3-hydroxybutyrate] [UHMW-P(3HB)] synthesized by genetically engineeredEscherichia coliis an environmentally friendly bioplastic material which can be processed into strong films or fibers. An operon of three genes (organized asphaCAB) encodes the essential proteins for the production of P(3HB) in the native producer,Ralstonia eutropha. The three genes of thephaCABoperon arephaC, which encodes the polyhydroxyalkanoate (PHA) synthase,phaA, which encodes a 3-ketothiolase, andphaB, which encodes an acetoacetyl coenzyme A (acetoacetyl-CoA) reductase. In this study, the effect of gene order of thephaCABoperon (phaABC,phaACB,phaBAC,phaBCA,phaCAB, andphaCBA) on an expression plasmid in genetically engineeredE. coliwas examined in order to determine the best organization to produce UHMW-P(3HB). The results showed that P(3HB) molecular weights and accumulation levels were both dependent on the order of thephagenes relative to the promoter. The most balanced production result was achieved in the strain harboring thephaBCAexpression plasmid. In addition, analysis of expression levels and activity for P(3HB) biosynthesis enzymes and of P(3HB) molecular weight revealed that the concentration of active PHA synthase had a negative correlation with P(3HB) molecular weight and a positive correlation with cellular P(3HB) content. This result suggests that the level of P(3HB) synthase activity is a limiting factor for producing UHMW-P(3HB) and has a significant impact on P(3HB) production.

Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes

2015 ◽  
Vol 180 ◽  
pp. 172-176 ◽  
Author(s):  
Javier A. Linares-Pastén ◽  
Ramin Sabet-Azad ◽  
Laura Pessina ◽  
Roya R.R. Sardari ◽  
Mohammad H.A. Ibrahim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Reuteri ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase

Low molecular weight chitosans—Preparation with the aid of pronase, characterization and their bactericidal activity towards Bacillus cereus and Escherichia coli

2007 ◽  
Vol 1770 (4) ◽  
pp. 495-505 ◽  
Author(s):  
Acharya B. Vishu Kumar ◽  
Mandyam C. Varadaraj ◽  
Lalitha R. Gowda ◽  
Rudrapatnam N. Tharanathan
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Bacillus Cereus ◽  
Bactericidal Activity ◽  
Low Molecular Weight

Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide

2016 ◽  
Vol 100 (18) ◽  
pp. 7877-7885 ◽  
Author(s):  
Haichan Huang ◽  
Xiaobo Liu ◽  
Shencong Lv ◽  
Weihong Zhong ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Recombinant Escherichia Coli

scholarly journals Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

2009 ◽  
Vol 76 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Björn Andreeßen ◽  
Alvin Brian Lange ◽  
Horst Robenek ◽  
Alexander Steinbüchel
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase ◽  
Fed Batch ◽  
Interesting Application ◽  
Serovar Typhimurium ◽  
Fed Batch Fermentation

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

Endogenous Ethanol Affects Biopolyester Molecular Weight in Recombinant Escherichia coli

2013 ◽  
Vol 8 (11) ◽  
pp. 2568-2576 ◽  
Author(s):  
Ayaka Hiroe ◽  
Manami Hyakutake ◽  
Nicholas M. Thomson ◽  
Easan Sivaniah ◽  
Takeharu Tsuge
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Endogenous Ethanol

Influence of pH on the Molecular Weight of Poly-3-hydroxybutyric Acid (P3HB) Produced by Recombinant Escherichia coli

2013 ◽  
Vol 170 (6) ◽  
pp. 1336-1347 ◽  
Author(s):  
Johanna Katherine Bocanegra ◽  
José Geraldo da Cruz Pradella ◽  
Luiziana Ferreira da Silva ◽  
Marilda Keico Taciro ◽  
Jose Gregório Cabrera Gomez
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Recombinant Escherichia Coli ◽  
Hydroxybutyric Acid ◽  
Influence Of Ph
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