Aqueous Solutions of Polyvinylsulfonic Acid: Phase Separation and Specific. Interactions with Ions, Viscosity, Conductance and Potentiometry

1959 ◽  
Vol 63 (5) ◽  
pp. 671-680 ◽  
Author(s):  
H. Eisenberg ◽  
G. Ram Mohan











Science ◽  
2019 ◽  
Vol 365 (6455) ◽  
pp. 825-829 ◽  
Author(s):  
Tae Hun Kim ◽  
Brian Tsang ◽  
Robert M. Vernon ◽  
Nahum Sonenberg ◽  
Lewis E. Kay ◽  
...  

Membraneless organelles involved in RNA processing are biomolecular condensates assembled by phase separation. Despite the important role of intrinsically disordered protein regions (IDRs), the specific interactions underlying IDR phase separation and its functional consequences remain elusive. To address these questions, we used minimal condensates formed from the C-terminal disordered regions of two interacting translational regulators, FMRP and CAPRIN1. Nuclear magnetic resonance spectroscopy of FMRP-CAPRIN1 condensates revealed interactions involving arginine-rich and aromatic-rich regions. We found that different FMRP serine/threonine and CAPRIN1 tyrosine phosphorylation patterns control phase separation propensity with RNA, including subcompartmentalization, and tune deadenylation and translation rates in vitro. The resulting evidence for residue-specific interactions underlying co–phase separation, phosphorylation-modulated condensate architecture, and enzymatic activity within condensates has implications for how the integration of signaling pathways controls RNA processing and translation.



2015 ◽  
Vol 17 (43) ◽  
pp. 28818-28829 ◽  
Author(s):  
Viviana C. P. da Costa ◽  
Onofrio Annunziata

Salt induces liquid–liquid phase separation (LLPS) of the aqueous solutions of a dendrimer. LLPS occurs by cooling at low salt concentration and by heating at high salt concentration.



2004 ◽  
Vol 37 (8) ◽  
pp. 2909-2918 ◽  
Author(s):  
Mitsuhiro Shibayama ◽  
Kohji Isono ◽  
Satoshi Okabe ◽  
Takeshi Karino ◽  
Michihiro Nagao


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