Characterization of the Geographical Origin of Pecorino Sardo Cheese by Casein Stable Isotope (13C/12C and15N/14N) Ratios and Free Amino Acid Ratios

2001 ◽  
Vol 49 (3) ◽  
pp. 1404-1409 ◽  
Author(s):  
Gavina Manca ◽  
Federica Camin ◽  
Gavina C. Coloru ◽  
Alessandra Del Caro ◽  
Daniela Depentori ◽  
...  
Keyword(s):  
Amino Acid ◽  
Stable Isotope ◽  
Free Amino Acid ◽  
Free Amino ◽  
Geographical Origin

Characterization of Shortday Onion Cultivars of 3 Pungency Levels with Flavor Precursor, Free Amino Acid, Sulfur, and Sugar Contents

2009 ◽  
Vol 74 (6) ◽  
pp. C475-C480 ◽  
Author(s):  
Eun Jin Lee ◽  
Kil Sun Yoo ◽  
John Jifon ◽  
Bhimanagouda S. Patil
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino

Characterization of a leucine aminopeptidase of Babesia gibsoni

Parasitology ◽  
2009 ◽  
Vol 136 (9) ◽  
pp. 945-952 ◽  
Author(s):  
H. JIA ◽  
M. A. TERKAWI ◽  
G. O. ABOGE ◽  
Y.-K. GOO ◽  
Y. LUO ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Drug Targets ◽  
Leucine Aminopeptidase ◽  
Divalent Cations ◽  
Specific Inhibitor ◽  
Babesia Gibsoni ◽  
Aminopeptidase A

SUMMARYPeptidases of parasitic protozoa are currently under intense investigation in order to identify novel virulence factors, drug targets, and vaccine candidates, except in Babesia. Leucine aminopeptidases in protozoa, such as Plasmodium and Leishmania, have been identified to be involved in free amino acid regulation. We report here the molecular and enzymatic characterization, as well as the localization of a leucine aminopeptidase, a member of the M17 cytosolic aminopeptidase family, from B. gibsoni (BgLAP). A functional recombinant BgLAP (rBgLAP) expressed in Escherichia coli efficiently hydrolysed synthetic substrates for aminopeptidase, a leucine substrate. Enzyme activity of the rBgLAP was found to be optimum at pH 8·0 and at 37°C. The substrate profile was slightly different from its homologue in P. falciprum. The activity was also strongly dependent on metal divalent cations, and was inhibited by bestatin, which is a specific inhibitor for metalloprotease. These results indicated that BgLAP played an important role in free amino acid regulation.

scholarly journals Micronutrient composition and microbial community analysis across diverse landraces of the Ethiopian orphan crop enset

2019 ◽  
Author(s):  
Solomon Tamrat ◽  
James S. Borrell ◽  
Manosh K. Biswas ◽  
Dawd Gashu ◽  
Tigist Wondimu ◽  
...  
Keyword(s):  
Food Security ◽  
Microbial Community ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Genomic Analysis ◽  
Community Analysis ◽  
Nutritional Composition ◽  
Microbial Community Analysis

AbstractEnset (Ensete ventricosum) is a major starch staple and food security crop for 20 million people. Despite substantial diversity in morphology, genetics, agronomy and utilization across its range, nutritional characteristics have only been reported in relatively few landraces. Here, we survey nutritional composition in 22 landraces from three enset growing regions. We present mineral characterization of enset corm tissue, free amino acid characterization of raw and processed (fermented) tissues and genomic analysis of the microbial community associated with fermentation. We show that compared to regionally important tubers and cereals, enset is high in calcium, iron, potassium and zinc and low in sodium. We report changes in free amino acid composition due to processing, and establish that the bacteria generaAcetobacter,LactobacillusandBifidobacterium, predominate during fermentation. Nutritional and microbial variation presents opportunities to select for improved composition, quality or safety with potentially significant impacts in food security and public health.

Characterization of free amino acid QTLs in maize opaque2 recombinant inbred lines

2011 ◽  
Vol 53 (2) ◽  
pp. 250-258 ◽  
Author(s):  
Karen V. Pineda-Hidalgo ◽  
Marilyn Lavin-Aramburo ◽  
Nancy Y. Salazar-Salas ◽  
Jeanett Chavez-Ontiveros ◽  
Cuauhtemoc Reyes-Moreno ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Recombinant Inbred ◽  
Free Amino ◽  
Recombinant Inbred Lines ◽  
Inbred Lines

scholarly journals Characterization of monofunctional aspartate kinase genes in maize and their relationship with free amino acid content in the endosperm

2007 ◽  
Vol 58 (10) ◽  
pp. 2653-2660 ◽  
Author(s):  
X. Wang ◽  
J. A. Lopez-Valenzuela ◽  
B. C. Gibbon ◽  
B. Gakiere ◽  
G. Galili ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Amino Acid Content ◽  
Aspartate Kinase ◽  
Free Amino Acid Content

The effect of steaming on the free amino acid contents and antioxidant activity of ginseng

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
EJ Cho ◽  
XL Piao ◽  
MH Jang ◽  
SY Park ◽  
SW Kwon ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Amino Acid Contents
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