Tissue residue studies with ronidazole. Effect of label site on total radioactivity content of rat tissues

1984 ◽  
Vol 32 (4) ◽  
pp. 706-711 ◽  
Author(s):  
Frank J. Wolf ◽  
Raul Alvaro ◽  
Lee R. Chapin ◽  
Marilyn L. Green ◽  
Donald E. Wolf ◽  
...  
Keyword(s):  
Total Radioactivity ◽  
Rat Tissues ◽  
Tissue Residue ◽  
Label Site ◽  
Radioactivity Content

IN VIVO UPTAKE AND METABOLISM OF 3H-5α-ANDROSTANE-3α, 17β-DIOL AND OF 3H-5α-ANDROSTANE-3β,17β-DIOL BY HUMAN PROSTATIC HYPERTROPHY

1975 ◽  
Vol 79 (2) ◽  
pp. 394-402 ◽  
Author(s):  
H.-J. Horst ◽  
M. Dennis ◽  
J. Kaufmann ◽  
K. D. Voigt
Keyword(s):  
Specific Radioactivity ◽  
Benign Prostatic Hypertrophy ◽  
Total Radioactivity ◽  
Prostatic Hypertrophy ◽  
Cellular Composition ◽  
Weak Correlation ◽  
Uptake And Metabolism ◽  
Radioactivity Content ◽  
In Vivo Uptake

ABSTRACT Tritiated 5α-androstane-3α,17β-diol (3α-diol) and 5α-androstane-3β,17β-diol (3β-diol) respectively were administered to patients with benign prostatic hypertrophy (bph) undergoing prostatectomy. In prostate and skeletal muscle homogenates and in plasma the total radioactivity content as well as the formation of metabolites were measured. Histological examination of each ectomized prostate was performed to evaluate the cellular composition of the tissue. After 3α-diol injection, a higher uptake of radioactivity in the prostate was obtained than after 3β-diol. Within 30 min the 3α-isomer was very efficiently converted to 5α-DHT, while most of the 3β-isomer remained unchanged. There was, however, also after administration of the 3β-diol a substantial biconversion to 5α-DHT as has been confirmed by recrystallization to constant specific radioactivity. Only after 3β-diol epiandrosterone was detected in small but significant amounts. 3α-diol administration resulted in distinct concentrations of 3β-diol, whereas the conversion of 3β-diol to the 3α-isomer was insignificant. When comparing the histological composition of the prostatic tissue with the accumulation of radioactivity and the formation of metabolites only a weak correlation between glandular structure and radioactivity uptake after 3α-diol administration could be revealed.

High-resolution scanning electron microscopy (HRSEM) of mitochondria from various rat tissues

1988 ◽  
Vol 46 ◽  
pp. 14-15
Author(s):  
P.J. Lea ◽  
M.J. Hollenberg
Keyword(s):  
Electron Microscopy ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Rat Hepatocytes ◽  
Three Dimensions ◽  
Objective Lens ◽  
Rat Tissues ◽  
Thin Sections ◽  
Reconstruction Methods ◽  
Scanning Electron

Our current understanding of mitochondrial ultrastructure has been derived primarily from thin sections using transmission electron microscopy (TEM). This information has been extrapolated into three dimensions by artist's impressions (1) or serial sectioning techniques in combination with computer processing (2). The resolution of serial reconstruction methods is limited by section thickness whereas artist's impressions have obvious disadvantages.In contrast, the new techniques of HRSEM used in this study (3) offer the opportunity to view simultaneously both the internal and external structure of mitochondria directly in three dimensions and in detail.The tridimensional ultrastructure of mitochondria from rat hepatocytes, retinal (retinal pigment epithelium), renal (proximal convoluted tubule) and adrenal cortex cells were studied by HRSEM. The specimens were prepared by aldehyde-osmium fixation in combination with freeze cleavage followed by partial extraction of cytosol with a weak solution of osmium tetroxide (4). The specimens were examined with a Hitachi S-570 scanning electron microscope, resolution better than 30 nm, where the secondary electron detector is located in the column directly above the specimen inserted within the objective lens.

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

Fibrinolytic Activity of Lung and Spleen Extracts Observed in Conventional but not in Germ-Free Rats

1979 ◽  
Vol 42 (02) ◽  
pp. 726-733 ◽  
Author(s):  
Utako Okamoto ◽  
Jun-ichiro Yamamoto ◽  
Yoko Nagamatsu ◽  
Noboru Horie
Keyword(s):  
Serine Protease ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Iodoacetic Acid ◽  
Thrombin Inhibitor ◽  
Rat Tissues ◽  
Germ Free ◽  
Neutral Ph ◽  
Tissue Extracts

SummaryProtease-like activity which split plasminogen-free fibrin was demonstrated in 2 M KSCN extracts of the lung and spleen of conventional rats. The activity was virtually undetectable in tissue extracts from germ-free rats. The extracts from the conventional rat tissues split fibrin and fibrinogen remarkably at neutral pH, but not casein, when examined using fibrin, fibrinogen-agar and casein-agar plates. The fibrinolytic activity was inhibited by STI and DFP, indicating a serine protease nature. The activity was not inhibited by TLCK, t-AMCHA or dansyl-L-arginine-methylpiperidine amide (a selective synthetic thrombin inhibitor, OM189). It was neither activated nor inhibited by cysteine, KCN or iodoacetic acid. The results obtained indicate that the protease-like activity of the lung and spleen extracted with 2 M KSCN from conventional rats has properties which differ from those of trypsin, plasmin, plasminogen-activator, thrombin, and cathepsin A, B and C.

Melatonin actions in the heart; more than a hormone

2018 ◽  
Vol 1 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Darío Acuña-Castroviejo ◽  
Maria T Noguiera-Navarro ◽  
Russel J Reiter ◽  
Germaine Escames
Keyword(s):  
Cell Membranes ◽  
Myocardial Cell ◽  
Rat Tissues ◽  
Dependent Manner ◽  
Broad Distribution ◽  
Multiple Organs ◽  
High Doses ◽  
Extrapineal Melatonin ◽  
Dose Dependent ◽  
Different Doses

Due to the broad distribution of extrapineal melatonin in multiple organs and tissues, we analyzed the presence and subcellular distribution of the indoleamine in the heart of rats. Groups of sham-operated and pinealectomized rats were sacrificed at different times along the day, and the melatonin content in myocardial cell membranes, cytosol, nuclei and mitochondria, were measured. Other groups of control animals were treated with different doses of melatonin to monitor its intracellular distribution. The results show that melatonin levels in the cell membrane, cytosol, nucleus, and mitochondria vary along the day, without showing a circadian rhythm. Pinealectomized animals trend to show higher values than sham-operated rats. Exogenous administration of melatonin yields its accumulation in a dose-dependent manner in all subcellular compartments analyzed, with maximal concentrations found in cell membranes at doses of 200 mg/kg bw melatonin. Interestingly, at dose of 40 mg/kg b.w, maximal concentration of melatonin was reached in the nucleus and mitochondrion. The results confirm previous data in other rat tissues including liver and brain, and support that melatonin is not uniformly distributed in the cell, whereas high doses of melatonin may be required for therapeutic purposes.

Regulation of insulin receptor mRNA splicing in rat tissues. Effect of fasting, aging, and diabetes

Diabetes ◽  
1995 ◽  
Vol 44 (10) ◽  
pp. 1196-1201 ◽  
Author(s):  
H. Vidal ◽  
D. Auboeuf ◽  
M. Beylot ◽  
J. P. Riou
Keyword(s):  
Insulin Receptor ◽  
Mrna Splicing ◽  
Rat Tissues ◽  
Receptor Mrna

Familial And Constitutional Moderate Bleeding Disorder Associated With Defective Platelet Cyclo-Oxygenase

1981 ◽  
Author(s):  
C Lecrubier ◽  
F Fouque ◽  
M Chignard ◽  
M H Horellou ◽  
J Conard ◽  
...  
Keyword(s):  
Autosomal Dominant ◽  
Atp Release ◽  
Rabbit Aorta ◽  
Total Radioactivity ◽  
Bleeding Disorder ◽  
Similar Extent ◽  
Synthetase Deficiency ◽  
Successive Generations ◽  
Second Wave ◽  
Rule Out

Three family members from two successive generations showed a moderate bleeding disorder. The bleeding times (Duke and Ivy-Borchgrevink) were both constantly prolonged. Platelet aggregation induced by ADP and adrenaline showed no second wave; collagen at low to moderate concentrations failed to aggregate and release ATP whereas higher amounts aggregated and released. Aggregation and release due to thrombin, ristocetin and synthetic epoxy derivatives (U 44069 and U 46619) were normal. Arachidonate (A.A.) was inactive and was not converted into thromboxane (TX) A2 activity evaluated on the rabbit aorta strip. The patient’s platelets did not respond to A.A. when PRP from an aspirin treated control was added. Platelet phospholipids (PL) were labelled by 14C - A.A. before stimulation by thrombin (T). Radioactivity of the PL of the propositus' platelets was affected with T to a similar extent as 5 laboratory controls, indicating that the phospholipase activity was not impaired. In contrast, no TXB2 was found: 0.9 p. cent as compared to 6 p. cent of total radioactivity in controls.Our data suggest a deficit in cyclo-oxygenase particularly since the levels of PGE2, PGF2a and PGD2 were within the limits of detection. These results seem to rule out the possibility of a TX-synthetase deficiency with excessive production of anti-aggregating PGs. This study suggests that transmission is autosomal dominant and confirms that cyclo-oxygenase is not needed for aggregation and ATP-release by high amounts of collagen.

scholarly journals Developmental regulation of insulin-like growth factor II mRNA in different rat tissues.

1986 ◽  
Vol 261 (28) ◽  
pp. 13144-13150 ◽  
Author(s):  
A L Brown ◽  
D E Graham ◽  
S P Nissley ◽  
D J Hill ◽  
A J Strain ◽  
...  
Keyword(s):  
Growth Factor ◽  
Developmental Regulation ◽  
Rat Tissues ◽  
Insulin Like Growth Factor ◽  
Factor Ii

scholarly journals Distribution of prenyltransferases in rat tissues. Evidence for a cytosolic all-trans-geranylgeranyl diphosphate synthase.

1993 ◽  
Vol 268 (2) ◽  
pp. 832-838
Author(s):  
J. Ericsson ◽  
M. Runquist ◽  
A. Thelin ◽  
M. Andersson ◽  
T. Chojnacki ◽  
...  
Keyword(s):  
Rat Tissues ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate
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