Heterogeneous Cationic Liposomes Modified with 3β-{N-[(N′,N′-Dimethylamino)ethyl]carbamoyl}cholesterol Can Induce Partial Conformational Changes in Messenger RNA and Regulate Translation in an Escherichia coli Cell-Free Translation System

Langmuir ◽  
2013 ◽  
Vol 29 (6) ◽  
pp. 1899-1907 ◽  
Author(s):  
Keishi Suga ◽  
Tomoyuki Tanabe ◽  
Hiroshi Umakoshi
Keyword(s):  
Escherichia Coli ◽  
Conformational Changes ◽  
Messenger Rna ◽  
Cationic Liposomes ◽  
Coli Cell ◽  
Translation System ◽  
Free Translation

Stabilization of mRNA in an Escherichia coli cell-free translation system

FEBS Letters ◽  
1993 ◽  
Vol 321 (2-3) ◽  
pp. 169-172 ◽  
Author(s):  
Ichiro Hirao ◽  
Satoko Yoshizawa ◽  
Kin-ichiro Miura
Keyword(s):  
Escherichia Coli ◽  
Coli Cell ◽  
Translation System ◽  
Free Translation

scholarly journals A single uridine modification at the wobble position of an artificial tRNA enhances wobbling in an Escherichia coli cell-free translation system

FEBS Letters ◽  
1999 ◽  
Vol 447 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Kazuyuki Takai ◽  
Shuhei Okumura ◽  
Kazumi Hosono ◽  
Shigeyuki Yokoyama ◽  
Hiroshi Takaku
Keyword(s):  
Escherichia Coli ◽  
Coli Cell ◽  
Translation System ◽  
Wobble Position ◽  
Free Translation

Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system

1999 ◽  
Vol 88 (6) ◽  
pp. 605-609 ◽  
Author(s):  
Junhao Yang ◽  
Koei Kobayashi ◽  
Hideo Nakano ◽  
Junko Tanaka ◽  
Takuya Nihira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coli Cell ◽  
Translation System ◽  
Pseudomonas Sp ◽  
Active Lipase

scholarly journals Effects of anticodon 2′-O-methylations on tRNA codon recognition in an Escherichia coli cell-free translation

RNA ◽  
2000 ◽  
Vol 6 (5) ◽  
pp. 680-686 ◽  
Author(s):  
AKIRA SATOH ◽  
KAZUYUKI TAKAI ◽  
RYOSUKE OUCHI ◽  
SHIGEYUKI YOKOYAMA ◽  
HIROSHI TAKAKU
Keyword(s):  
Escherichia Coli ◽  
Coli Cell ◽  
Codon Recognition ◽  
Free Translation

Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa and translation of pyruvate kinase messenger RNA

1984 ◽  
Vol 4 (11) ◽  
pp. 979-986 ◽  
Author(s):  
M. Devchand ◽  
M. Kapoor
Keyword(s):  
Neurospora Crassa ◽  
Messenger Rna ◽  
In Vitro Translation ◽  
Translation System ◽  
Wild Type ◽  
Post Translational Modification ◽  
Globin Mrna ◽  
Free Translation ◽  
A Cell

A cell-free in vitro translation system exhibiting high activity has been developed from wild-type Neurospora crassa mycelium. The isolation is simple and fast, and the homogenization does not appear to affect the activity of mycelial proteases and nucleases. This system is capable of supporting efficient translation of exogenously added homologous RNA as demonstrated by the experiments with PK-specific mRNA. In addition, it translates heterologous RNA efficiently, shown by the translation of globin mRNA. We did not examine the Neurospora lysate for post-translational modification activity. The procedure used for the preparation of Neurospora cell-free extracts should be readily applicable to the other filamentous fungi.

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