Colorimetric Detection of Lysozyme Based on Electrostatic Interaction with Human Serum Albumin-Modified Gold Nanoparticles

A systematic investigation on the effect of gold nanoparticle morphology and surface functionalization on the differential interaction of HSA was performed.

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Flow-injection with enhanced evaporative light scattering detector detection and quantification of human serum albumin using gold nanoparticles

Analytical Methods ◽

10.1039/c4ay02918f ◽

2015 ◽

Vol 7 (7) ◽

pp. 3185-3192 ◽

Cited By ~ 2

Author(s):

Tao Wang ◽

Mengbin Chen ◽

Caixia Niu ◽

Ning Liu ◽

Le Wang ◽

...

Keyword(s):

Gold Nanoparticles ◽

Light Scattering ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Flow Injection ◽

Evaporative Light Scattering Detector ◽

Evaporative Light Scattering ◽

Detection And Quantification

The ELSD response for HSA was enhanced by using GNPs, making it possible to determine HSA by ELSD.

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Multiwall carbon nanotube-ionic liquid electrode modified with gold nanoparticles as a base for preparation of a novel impedimetric immunosensor for low level detection of human serum albumin in biological fluids

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2014.01.005 ◽

2014 ◽

Vol 92 ◽

pp. 74-81 ◽

Cited By ~ 33

Author(s):

Elham Arkan ◽

Reza Saber ◽

Ziba Karimi ◽

Ali Mostafaie ◽

Mojtaba Shamsipur

Keyword(s):

Gold Nanoparticles ◽

Ionic Liquid ◽

Carbon Nanotube ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Biological Fluids ◽

Multiwall Carbon Nanotube ◽

Impedimetric Immunosensor ◽

Multiwall Carbon

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Polyphenol capping on a gold nanosurface modulates human serum albumin fibrillation

Materials Advances ◽

10.1039/d0ma00274g ◽

2020 ◽

Vol 1 (5) ◽

pp. 1142-1150

Author(s):

Aalok Basu ◽

Sonia Kundu ◽

Aatrayee Das ◽

Chitra Basu ◽

Sagar Bhayye ◽

...

Keyword(s):

Gold Nanoparticles ◽

Human Serum Albumin ◽

Serum Albumin ◽

Antioxidant Capacity ◽

Human Serum

The antioxidant capacity inherent to polyphenol-capped gold nanoparticles contributed to the inhibition of human serum albumin fibrillation.

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At very low concentrations known chaotropes act as kosmotropes for the N and B isoforms of human serum albumin

Biochemistry and Cell Biology ◽

10.1139/bcb-2012-0035 ◽

2013 ◽

Vol 91 (2) ◽

pp. 72-78 ◽

Cited By ~ 5

Author(s):

Priyankar Sen ◽

Mohd Moin Khan ◽

Asif Equbal ◽

Ejaz Ahmad ◽

Rizwan Hasan Khan

Keyword(s):

Protein Structure ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Electrostatic Interaction ◽

Tertiary Structure ◽

Guanidine Hydrochloride ◽

Micelle Formation ◽

Low Concentrations ◽

Secondary And Tertiary Structure

Very few studies have been done to understand the effect of millimolar concentrations of chaotropes on protein structure. In our previous study we observed that the secondary and tertiary structure of human serum albumin (HSA) increases in the presence of 5 mmol/L urea. Micelle formation in amphoteric detergents increases in the presence of equivalent concentrations of urea. Here, we observed a significant increase in the secondary and tertiary structure of HSA. Interestingly, guanidine hydrochloride, another chaotropic agent, also shows a similar effect. Our results show electrostatic interaction may play a role in neutral to basic transition in HSA. This study further supports the claim that at millimolar concentrations the chaotropes may act as kosmotropes for proteins.

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