The Effects of Mannitol on the Transport of Ciprofloxacin across Respiratory Epithelia

2013 ◽  
Vol 10 (8) ◽  
pp. 2915-2924 ◽  
Author(s):  
Hui Xin Ong ◽  
Daniela Traini ◽  
Rania Salama ◽  
Sandra D. Anderson ◽  
Evangelia Daviskas ◽  
...  
Keyword(s):  
Respiratory Epithelia

Methods for making stereoslides and -prints, with freeze-etched olfactory epithelium as example

1984 ◽  
Vol 42 ◽  
pp. 704-705
Author(s):  
Bert Ph. M. Menco ◽  
Ido F. Menco ◽  
Frans L.T. Verdonk
Keyword(s):  
Electron Microscope ◽  
Olfactory Epithelium ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Structural Features ◽  
Extensive Study ◽  
Sprague Dawley ◽  
Freezing Method ◽  
Respiratory Epithelia ◽  
Three Dimensional Presentation

Previously we presented an extensive study of the distributions of intramembranous particles of structures in apical surfaces of nasal olfactory and respiratory epithelia of the Sprague-Dawley rat. For the same structures these distributions were compared in samples which were i) chemically fixed and cryo-protected with glycerol before cryo-fixation, after excision, and ii)ultra-rapidly frozen by means of the slam-freezing method. Since a three-dimensional presentation markedly improves visualization of structural features micrographs were presented as stereopairs. Two exposures were made by tiling the sample stage of the electron microscope 6° in either direction with an eucentric goniometer. The negatives (Agfa Pan 25 Professional) were reversed with Kodak Technical Pan Film 2415 developed in D76 1:1. The prints were made from these reversed negatives. As an example tight-junctional features of an olfactory supporting cell in a region where this cell conjoined with two other cells are presented (Fig. 1).

scholarly journals Regulation of flagellar motility by the conserved flagellar protein CG34110/Ccdc135/FAP50

2011 ◽  
Vol 22 (7) ◽  
pp. 976-987 ◽  
Author(s):  
Yong Yang ◽  
Deborah A. Cochran ◽  
Mary D. Gargano ◽  
Iryna King ◽  
Nayef K. Samhat ◽  
...  
Keyword(s):  
Flagellar Motility ◽  
Genetic Screens ◽  
Sperm Flagellum ◽  
Respiratory Epithelia ◽  
Downstream Effectors ◽  
Radial Spokes ◽  
Flagellar Proteins ◽  
Flagellar Protein ◽  
Cilia And Flagella ◽  
Lost Boys

Eukaryotic cilia and flagella are vital sensory and motile organelles. The calcium channel PKD2 mediates sensory perception on cilia and flagella, and defects in this can contribute to ciliopathic diseases. Signaling from Pkd2-dependent Ca2+ rise in the cilium to downstream effectors may require intermediary proteins that are largely unknown. To identify these proteins, we carried out genetic screens for mutations affecting Drosophila melanogaster sperm storage, a process mediated by Drosophila Pkd2. Here we show that a new mutation lost boys (lobo) encodes a conserved flagellar protein CG34110, which corresponds to vertebrate Ccdc135 (E = 6e-78) highly expressed in ciliated respiratory epithelia and sperm, and to FAP50 (E = 1e-28) in the Chlamydomonas reinhardtii flagellar proteome. CG34110 localizes along the fly sperm flagellum. FAP50 is tightly associated with the outer doublet microtubules of the axoneme and appears not to be a component of the central pair, radial spokes, dynein arms, or structures defined by the mbo waveform mutants. Phenotypic analyses indicate that both Pkd2 and lobo specifically affect sperm movement into the female storage receptacle. We hypothesize that the CG34110/Ccdc135/FAP50 family of conserved flagellar proteins functions within the axoneme to mediate Pkd2-dependent processes in the sperm flagellum and other motile cilia.

scholarly journals A novel isoform of ACE2 is expressed in human nasal and bronchial respiratory epithelia and is upregulated in response to RNA respiratory virus infection

2020 ◽  
Author(s):  
Cornelia Blume ◽  
Claire L Jackson ◽  
Cosma Mirella Spalluto ◽  
Jelmer Legebeke ◽  
Liliya Nazlamova ◽  
...  
Keyword(s):  
Critical Role ◽  
Host Susceptibility ◽  
Viral Fusion ◽  
Angiotensin Converting Enzyme 2 ◽  
Main Site ◽  
Respiratory Epithelia ◽  
Acid Protein ◽  
Asthma Patients ◽  
Respiratory Virus Infection ◽  
Amino Acid Protein

AbstractAngiotensin-converting enzyme 2 (ACE2) is the main entry point in the airways for SARS-CoV-2. ACE2 binding to SARS-CoV-2 protein Spike triggers viral fusion with the cell membrane, resulting in viral RNA genome delivery into the host. Despite ACE2’s critical role in SARS-CoV-2 infection, an understanding of ACE2 expression, including in response to viral infection, remains unclear.Until now ACE2 was thought to encode five transcripts and one 805 amino acid protein. Here we identify a novel short isoform of ACE2. Short ACE2 is expressed in the airway epithelium, the main site of SARS-CoV-2 infection; it is substantially upregulated in response to interferon stimulation and RV infection, but not in response to SARS-CoV-2 infection, and it shows differential regulation in asthma patients. This short isoform lacks SARS-CoV-2 spike glycoprotein high-affinity binding sites and altogether, our data are consistent with a model where short ACE2 may influence host susceptibility to SARS-CoV-2 infection.

Vitamin A and ciliated cells I. Respiratory epithelia

1986 ◽  
Vol 25 (2) ◽  
pp. 114-122 ◽  
Author(s):  
H. K. Biesalski ◽  
E. Stofft ◽  
U. Wellner ◽  
U. Niederauer ◽  
K. H. Bässler
Keyword(s):  
Vitamin A ◽  
Ciliated Cells ◽  
Respiratory Epithelia

Cell kinetics analysis in a case of teratoma of the thoracic spine

1986 ◽  
Vol 65 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Judith A. Murovic ◽  
Stephen De Armond ◽  
Tadashi Nagashima ◽  
Michael S. B. Edwards ◽  
Takao Hoshino
Keyword(s):  
Deoxyribonucleic Acid ◽  
Cell Kinetics ◽  
Mature Teratoma ◽  
Basal Layer ◽  
Growth Patterns ◽  
Content Type ◽  
Respiratory Epithelia ◽  
Mesenchymal Tissue ◽  
Thoracic Spinal ◽  
Cell Layers

✓ The authors report cell kinetics studies in an infant who had multiple operations for removal of a rare benign thoracic spinal teratoma with retroperitoneal extension. Before the final surgical procedure for recurrent tumor, bromodeoxyuridine (BUdR), 200 mg/sq m, was administered intravenously to label tumor cells in the S (deoxyribonucleic acid (DNA) synthesis) phase of the cell cycle. Histologically, the tumor was a mature teratoma consisting of components derived from all three germ-cell layers. Cells labeled with BUdR were found in the basal layer of stratified squamous epithelia, in respiratory epithelia, in the cartilage and surrounding perichondrial mesenchyme, and in loose mesenchymal tissue throughout the teratoma. In contrast to neuroec-todermal tumors, which show widespread BUdR uptake throughout the tissue and which have different average labeling indices according to their histological type (range < 1 % to 15.2%), the teratoma showed BUdR labeling only in certain areas, indicating fairly organized growth patterns; the labeling indices in these areas ranged from 0.39% to 1.9%.

mRNA-induction and cytokine release during in vitro exposure of human nasal respiratory epithelia to methyl methacrylate

2007 ◽  
Vol 171 (1-2) ◽  
pp. 29-37 ◽  
Author(s):  
Jan Gosepath ◽  
Juergen Brieger ◽  
Axel Muttray ◽  
Sandra Best ◽  
Mehrdad Pourianfar ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Cytokine Release ◽  
Mrna Induction ◽  
Respiratory Epithelia ◽  
In Vitro Exposure

Evaluation of DNA damage by the alkaline comet assay of the olfactory and respiratory epithelia of dogs from the city of São Paulo, Brazil

2010 ◽  
Vol 62 (3) ◽  
pp. 209-219 ◽  
Author(s):  
K.C. Kimura ◽  
H. Fukumasu ◽  
L.M. Chaible ◽  
C.E. Lima ◽  
M.A. Horst ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Sao Paulo ◽  
São Paulo ◽  
Alkaline Comet Assay ◽  
Respiratory Epithelia ◽  
The City

Regeneration of respiratory epithelia in the rat after free grafting

1992 ◽  
Vol 249 (7) ◽  
Author(s):  
F. Bootz ◽  
G. Reuter
Keyword(s):  
Respiratory Epithelia ◽  
Free Grafting
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