Microfluidic Analysis of Antibody Specificity in a Compact Disk Format

2006 ◽  
Vol 5 (7) ◽  
pp. 1568-1574 ◽  
Author(s):  
Cecilia Eriksson ◽  
Charlotta Agaton ◽  
Rikard Kånge ◽  
Mårten Sundberg ◽  
Peter Nilsson ◽  
...  
Keyword(s):  
Antibody Specificity ◽  
Compact Disk ◽  
Microfluidic Analysis

scholarly journals Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 558
Author(s):  
Hwee-Yeong Ng ◽  
Wen-Chin Lee ◽  
Chia-Te Kung ◽  
Lung-Chih Li ◽  
Chien-Te Lee ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Human Life ◽  
Microfluidic Devices ◽  
Quality Inspection ◽  
Milk Samples ◽  
Sample Preconcentration ◽  
Sample Extraction ◽  
Microfluidic Analysis ◽  
Sample Separation

Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

Auditory demonstrations on compact disk for large N

1993 ◽  
Vol 93 (1) ◽  
pp. 1-16 ◽  
Author(s):  
William Morris Hartmann
Keyword(s):  
Compact Disk ◽  
Large N

scholarly journals Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
G. Wirobski ◽  
F. S. Schaebs ◽  
F. Range ◽  
S. Marshall-Pescini ◽  
T. Deschner
Keyword(s):  
Enzyme Immunoassay ◽  
Human Urine ◽  
Extraction Efficiency ◽  
Urine Samples ◽  
Antibody Specificity ◽  
Analytical Validation ◽  
Human Urine Samples ◽  
Assay Performance ◽  
Fit For Purpose ◽  
Commercial Enzyme Immunoassay

AbstractOxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of analytical methods and an assessment of the physiological significance of these measures are essential. We conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit) to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay’s ability to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay performance with regard to parallelism was acceptable. Assay accuracy and extraction efficiency for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but variation was smaller for human samples. Binding sensitivity and antibody specificity were better in the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples measured with the Enzo assay, highlighting a lack of comparability of absolute values between different assays. Changes in OTM concentrations after intranasal treatment were detected reliably. The Arbor assay met requirements of a “fit-for-purpose” validation with improvement of several parameters compared to the Enzo assay.

Monoclonal Antibody Specificity: Orthoclone OKT3 T-Cell Blocker

Nephron ◽  
1987 ◽  
Vol 46 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Gideon Goldstein
Keyword(s):  
Monoclonal Antibody ◽  
T Cell ◽  
Antibody Specificity

Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

2014 ◽  
Vol 63 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Georg Härter ◽  
Hagen Frickmann ◽  
Sebastian Zenk ◽  
Dominic Wichmann ◽  
Bettina Ammann ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Symptoms ◽  
Diagnostic Procedures ◽  
Lower Limbs ◽  
Antibody Specificity ◽  
Quantitative Real Time Pcr ◽  
Specific Antibodies ◽  
Routine Diagnosis

We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum–cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

Quantitative microfluidic analysis ofS- andR-type endotoxin components with chip capillary electrophoresis

2012 ◽  
Vol 33 (22) ◽  
pp. 3351-3360 ◽  
Author(s):  
Lilla Makszin ◽  
Anikó Kilár ◽  
Péter Felső ◽  
Zoltán Péterfi ◽  
Béla Kocsis ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Microfluidic Analysis

Triple-wavelength optical pickup head designs for compact disk, digital versatile disk and high-density digital versatile disk devices

2005 ◽  
Vol 52 (17) ◽  
pp. 2523-2547 ◽  
Author(s):  
Wen-Shing Sun ◽  
Ching-Cherng Sun * ◽  
Jen-Tsorng Chang ◽  
Chuen-Lin Tien ◽  
Shih-Hsin Ma ◽  
...  
Keyword(s):  
High Density ◽  
Compact Disk ◽  
Optical Pickup ◽  
Optical Pickup Head ◽  
Digital Versatile Disk
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