Proteome Profile of Cytosolic Component of Zebrafish Liver Generated by LC−ESI MS/MS Combined with Trypsin Digestion and Microwave-Assisted Acid Hydrolysis

2007 ◽  
Vol 6 (1) ◽  
pp. 263-272 ◽  
Author(s):  
Nan Wang ◽  
Lauren MacKenzie ◽  
Andrea G. De Souza ◽  
Hongying Zhong ◽  
Greg Goss ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Trypsin Digestion ◽  
Esi Ms ◽  
Microwave Assisted ◽  
Proteome Profile ◽  
Zebrafish Liver

Optimisation of microwave-assisted acid hydrolysis for the determination of seleno-amino acids bound to proteins in powdered milk, lyophilized milk and infant formula

2019 ◽  
Vol 79 ◽  
pp. 128-133
Author(s):  
Romina Lopez ◽  
Luis Escudero ◽  
Roberto D’Amato ◽  
Daniela Businelli ◽  
Massimo Trabalza-Marinucci ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acid Hydrolysis ◽  
Infant Formula ◽  
Powdered Milk ◽  
Microwave Assisted

Benefit of microwave-assisted acid hydrolysis of proteins for mass spectrometric profiling of the human heart tissue proteome

2007 ◽  
Vol 21 (16) ◽  
pp. 2779-2783 ◽  
Author(s):  
Mulu Gebremedhin ◽  
Hongying Zhong ◽  
Shaohua Wang ◽  
Michael Weinfeld ◽  
Liang Li
Keyword(s):  
Acid Hydrolysis ◽  
Human Heart ◽  
Heart Tissue ◽  
Mass Spectrometric ◽  
Microwave Assisted ◽  
Hydrolysis Of ◽  
Tissue Proteome ◽  
Human Heart Tissue

scholarly journals Proteome profile of functional mitochondria from human skeletal muscle using one-dimensional gel electrophoresis and HPLC-ESI-MS/MS

2009 ◽  
Vol 72 (6) ◽  
pp. 1046-1060 ◽  
Author(s):  
Natalie Lefort ◽  
Zhengping Yi ◽  
Benjamin Bowen ◽  
Brian Glancy ◽  
Eleanna A. De Filippis ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gel Electrophoresis ◽  
Human Skeletal Muscle ◽  
One Dimensional ◽  
Esi Ms ◽  
Proteome Profile

scholarly journals Optimization of Xylose Production from Sugarcane Trash by Microwave-Maleic Acid Hydrolysis

REAKTOR ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 81-88
Author(s):  
Euis Hermiati ◽  
Maulida Oktaviani ◽  
Riksfardini Annisa Ermawar ◽  
Raden Permana Budi Laksana ◽  
Lutfi Nia Kholida ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Acid Hydrolysis ◽  
Response Surface ◽  
Acid Concentration ◽  
Maleic Acid ◽  
Sugar Alcohol ◽  
Quadratic Regression ◽  
Microwave Assisted ◽  
Concentration Time ◽  
Xylose Production

Sugarcane trash contains significant amount of xylan that could be hydrolysed to xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has low calories and does not cause carries of teeth. In this study we optimized the production of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using response surface methodology (RSM). The factors optimized were acid concentration, time, and temperature. The xylose yield based on the weight of initial biomass was determined and it served as a response variable. Results show that acid concentration and interaction between time and temperature had significant effect on xylose yield. The quadratic regression model generated from the optimization was fit and can be used to predict the xylose yield after hydrolysis with various combinations of acid concentration, time, and temperature. The optimum condition for xylose production from sugarcane trash was using maleic acid of 1.52%, and heating at 176 °C for 6.8 min. At this condition the yield of xylose was 24.3% per initial biomass or 0.243 g/ g biomass.Keywords: maleic acid; microwave heating; response surface methodology; sugarcane trash, xylose

Preparation of κ-carra-oligosaccharides with microwave assisted acid hydrolysis method

2015 ◽  
Vol 14 (2) ◽  
pp. 345-349 ◽  
Author(s):  
Guangsheng Li ◽  
Xia Zhao ◽  
Youjing Lv ◽  
Miaomiao Li ◽  
Guangli Yu
Keyword(s):  
Acid Hydrolysis ◽  
Hydrolysis Method ◽  
Microwave Assisted

scholarly journals The Effects of Microwave-Assisted Pretreatment and Cofermentation on Bioethanol Production from Elephant Grass

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Sefrinus Maria Dolfi Kolo ◽  
Deana Wahyuningrum ◽  
Rukman Hertadi
Keyword(s):  
Acid Hydrolysis ◽  
Hydrolysis Product ◽  
Pichia Stipitis ◽  
Pennisetum Purpureum ◽  
Bioethanol Production ◽  
Elephant Grass ◽  
Microwave Assisted ◽  
Liquid Chromatographic Analysis ◽  
High Concentrations ◽  
Liquid Chromatographic

The process of acid hydrolysis using conventional methods at high concentrations results in products having lower yields, and it needs a longer time of process; therefore, it becomes less effective. In this study, we analyzed the effects of microwave-assisted pretreatment and cofermentation on bioethanol production from elephant grass (Pennisetum purpureum). We used a combination of delignification techniques and acid hydrolysis by employing a microwave-assisted pretreatment method on elephant grass (Pennisetum purpureum) as a lignocellulosic material. This was followed by cofermentation with Saccharomyces cerevisiae ITB-R89 and Pichia stipitis ITB-R58 to produce bioethanol. The optimal sugar mixtures (fructose and xylose) of the hydrolysis product were subsequently converted into bioethanol by cofermentation with S. cerevisiae ITB-R89 and P. stipitis ITB-R58, carried out with varying concentrations of inoculum for 5 days (48 h) at 30°C and pH 4.5. The high-power liquid chromatographic analysis revealed that the optimal inoculum concentration capable of converting 76.15% of the sugar mixture substrate (glucose and xylose) to 10.79 g/L (34.74% yield) of bioethanol was 10% (v/v). The optimal rate of ethanol production was 0.45 g/L/d, corresponding to a fermentation efficiency of 69.48%.

scholarly journals Microwave-assisted Acid Hydrolysis of Starch Polymer in Cassava Pulp in the Presence of Activated Carbon

2012 ◽  
Vol 4 ◽  
pp. 238-244 ◽  
Author(s):  
E. Hermiati ◽  
D. Mangunwidjaja ◽  
T.C. Sunarti ◽  
O. Suparno ◽  
B. Prasetya
Keyword(s):  
Activated Carbon ◽  
Acid Hydrolysis ◽  
Microwave Assisted ◽  
Cassava Pulp ◽  
Hydrolysis Of
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