Proteome Profile of Cytosolic Component of Zebrafish Liver Generated by LC−ESI MS/MS Combined with Trypsin Digestion and Microwave-Assisted Acid Hydrolysis

Sugarcane trash contains significant amount of xylan that could be hydrolysed to xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has low calories and does not cause carries of teeth. In this study we optimized the production of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using response surface methodology (RSM). The factors optimized were acid concentration, time, and temperature. The xylose yield based on the weight of initial biomass was determined and it served as a response variable. Results show that acid concentration and interaction between time and temperature had significant effect on xylose yield. The quadratic regression model generated from the optimization was fit and can be used to predict the xylose yield after hydrolysis with various combinations of acid concentration, time, and temperature. The optimum condition for xylose production from sugarcane trash was using maleic acid of 1.52%, and heating at 176 °C for 6.8 min. At this condition the yield of xylose was 24.3% per initial biomass or 0.243 g/ g biomass.Keywords: maleic acid; microwave heating; response surface methodology; sugarcane trash, xylose

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Preparation of κ-carra-oligosaccharides with microwave assisted acid hydrolysis method

Journal of Ocean University of China ◽

10.1007/s11802-015-2409-3 ◽

2015 ◽

Vol 14 (2) ◽

pp. 345-349 ◽

Cited By ~ 3

Author(s):

Guangsheng Li ◽

Xia Zhao ◽

Youjing Lv ◽

Miaomiao Li ◽

Guangli Yu

Keyword(s):

Acid Hydrolysis ◽

Hydrolysis Method ◽

Microwave Assisted

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Identification of bacteriocins secreted by the probiotic Lactococcus lactis following microwave-assisted acid hydrolysis (MAAH), amino acid content analysis, and bioinformatics

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-017-0770-3 ◽

2017 ◽

Vol 410 (4) ◽

pp. 1299-1310 ◽

Cited By ~ 6

Author(s):

Rafail Nikolaos Tasakis ◽

Maria Touraki

Keyword(s):

Content Analysis ◽

Amino Acid ◽

Acid Hydrolysis ◽

Lactococcus Lactis ◽

Acid Content ◽

Amino Acid Content ◽

Microwave Assisted

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The Effects of Microwave-Assisted Pretreatment and Cofermentation on Bioethanol Production from Elephant Grass

International Journal of Microbiology ◽

10.1155/2020/6562730 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Sefrinus Maria Dolfi Kolo ◽

Deana Wahyuningrum ◽

Rukman Hertadi

Keyword(s):

Acid Hydrolysis ◽

Hydrolysis Product ◽

Pichia Stipitis ◽

Pennisetum Purpureum ◽

Bioethanol Production ◽

Elephant Grass ◽

Microwave Assisted ◽

Liquid Chromatographic Analysis ◽

High Concentrations ◽

Liquid Chromatographic

The process of acid hydrolysis using conventional methods at high concentrations results in products having lower yields, and it needs a longer time of process; therefore, it becomes less effective. In this study, we analyzed the effects of microwave-assisted pretreatment and cofermentation on bioethanol production from elephant grass (Pennisetum purpureum). We used a combination of delignification techniques and acid hydrolysis by employing a microwave-assisted pretreatment method on elephant grass (Pennisetum purpureum) as a lignocellulosic material. This was followed by cofermentation with Saccharomyces cerevisiae ITB-R89 and Pichia stipitis ITB-R58 to produce bioethanol. The optimal sugar mixtures (fructose and xylose) of the hydrolysis product were subsequently converted into bioethanol by cofermentation with S. cerevisiae ITB-R89 and P. stipitis ITB-R58, carried out with varying concentrations of inoculum for 5 days (48 h) at 30°C and pH 4.5. The high-power liquid chromatographic analysis revealed that the optimal inoculum concentration capable of converting 76.15% of the sugar mixture substrate (glucose and xylose) to 10.79 g/L (34.74% yield) of bioethanol was 10% (v/v). The optimal rate of ethanol production was 0.45 g/L/d, corresponding to a fermentation efficiency of 69.48%.

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