Identification of Autocrine Growth Factors Secreted by CHO Cells for Applications in Single-Cell Cloning Media

2013 ◽  
Vol 12 (7) ◽  
pp. 3496-3510 ◽  
Author(s):  
U Ming Lim ◽  
Miranda Gek Sim Yap ◽  
Yoon Pin Lim ◽  
Lin-Tang Goh ◽  
Say Kong Ng
Keyword(s):  
Growth Factors ◽  
Single Cell ◽  
Cho Cells ◽  
Cell Cloning ◽  
Autocrine Growth ◽  
Autocrine Growth Factors ◽  
Single Cell Cloning

Measuring the aggregation of CHO cells prior to single cell cloning allows a more accurate determination of the probability of clonality

2017 ◽  
Vol 34 (3) ◽  
pp. 593-601 ◽  
Author(s):  
Kerensa J. Klottrup ◽  
Guillermo Miro-Quesada ◽  
Layla Flack ◽  
Ivan Pereda ◽  
Pamela Hawley-Nelson
Keyword(s):  
Single Cell ◽  
Cho Cells ◽  
Accurate Determination ◽  
Cell Cloning ◽  
Single Cell Cloning

Recombinant human albumin supports single cell cloning of CHO cells in chemically defined media

2012 ◽  
Vol 28 (3) ◽  
pp. 887-891 ◽  
Author(s):  
Jiang Zhu ◽  
Jong Wei Wooh ◽  
Jeff Jia Cheng Hou ◽  
Benjamin S. Hughes ◽  
Peter P. Gray ◽  
...  
Keyword(s):  
Single Cell ◽  
Cho Cells ◽  
Human Albumin ◽  
Cell Cloning ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Single Cell Cloning ◽  
Recombinant Human Albumin

Fibroblast Responses to Exogenous and Autocrine Growth Factors Relevant to Tissue Repair: The Effect of Aging

2006 ◽  
Vol 908 (1) ◽  
pp. 155-166 ◽  
Author(s):  
DIMITRIS KLETSAS ◽  
HARRIS PRATSINIS ◽  
IRENE ZERVOLEA ◽  
PANAGIOTIS HANDRIS ◽  
ELENI SEVASLIDOU ◽  
...  
Keyword(s):  
Growth Factors ◽  
Tissue Repair ◽  
Autocrine Growth ◽  
Autocrine Growth Factors

Secretion of autocrine growth factors by cultured human lung tumors: Effects on neoplastic lung epithelial cells

Lung Cancer ◽  
1988 ◽  
Vol 4 (3-4) ◽  
pp. 205-209 ◽  
Author(s):  
Jill M. Siegfried ◽  
Suzanne K. Hansen ◽  
Vickie Y. Lawrence ◽  
Sara E. Owens
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Tumors ◽  
Lung Epithelial Cells ◽  
Autocrine Growth ◽  
Autocrine Growth Factors ◽  
Lung Epithelial

scholarly journals An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Julia Wegner ◽  
Thomas Zillinger ◽  
Thais Schlee-Guimaraes ◽  
Eva Bartok ◽  
Martin Schlee
Keyword(s):  
Single Cell ◽  
Human Monocyte ◽  
Surface Expression ◽  
Human Monocytes ◽  
Innate Immune Responses ◽  
Loss Of Function ◽  
Cell Cloning ◽  
Gpi Anchor ◽  
Single Cell Cloning ◽  
Antigen Presenting

AbstractAntigen-presenting myeloid cells like monocytes detect invading pathogens via pattern recognition receptors (PRRs) and initiate adaptive and innate immune responses. As analysis of PRR signaling in primary human monocytes is hampered by their restricted expandability, human monocyte models like THP-1 cells are commonly used for loss-of-function studies, such as with CRISPR-Cas9 editing. A recently developed transdifferentiation cell culture system, BLaER1, enables lineage conversion from malignant B cells to monocytes and was found superior to THP-1 in mimicking PRR signaling, thus being the first model allowing TLR4 and inflammasome pathway analysis. Here, we identified an important caveat when investigating TLR4-driven signaling in BLaER1 cells. We show that this model contains glycosylphosphatidylinositol (GPI) anchor-deficient cells, which lack CD14 surface expression when differentiated to monocytes, resulting in diminished LPS/TLR4 but not TLR7/TLR8 responsiveness. This GPI anchor defect is caused by epigenetic silencing of PIGH, leading to a random distribution of intact and PIGH-deficient clones after single-cell cloning. Overexpressing PIGH restored GPI-anchored protein (including CD14) expression and LPS responsiveness. When studying CD14- or other GPI-anchored protein-dependent pathways, researchers should consider this anomaly and ensure equal GPI-anchored protein expression when comparing cells that have undergone single-cell cloning, e. g. after CRISPR-Cas9 editing.

scholarly journals Single cell cloning and recombinant monoclonal antibodies generation from RA synovial B cells reveal frequent targeting of citrullinated histones of NETs

2015 ◽  
Vol 75 (10) ◽  
pp. 1866-1875 ◽  
Author(s):  
Elisa Corsiero ◽  
Michele Bombardieri ◽  
Emanuela Carlotti ◽  
Federico Pratesi ◽  
William Robinson ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cells ◽  
Single Cell ◽  
Cell Cloning ◽  
Single Cell Cloning

scholarly journals Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cells

2017 ◽  
Vol 293 (3) ◽  
pp. 906-919 ◽  
Author(s):  
Tao Huang ◽  
Mary Mathieu ◽  
Sophia Lee ◽  
Xinhua Wang ◽  
Yee Seir Kee ◽  
...  
Keyword(s):  
B Cells ◽  
Single Cell ◽  
Molecular Characterization ◽  
Cell Cloning ◽  
Human B Cells ◽  
Single Cell Cloning ◽  
Normal Human

Serum changes the response of cultured morris hepatoma 7777 cells to autocrine growth factors

1990 ◽  
Vol 46 (1) ◽  
pp. 145-150
Author(s):  
Krzysztof Guzik ◽  
Andrzej Klein
Keyword(s):  
Growth Factors ◽  
Autocrine Growth ◽  
Autocrine Growth Factors ◽  
Morris Hepatoma
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