Toxicity and degradation of metal-complexed cyanide by a bacterial consortium under sulfate-reducing conditions

2004 ◽  
Vol 26 (12) ◽  
pp. 1007-1011 ◽  
Author(s):  
Zhe-Xue Quan ◽  
Jin-Woo Bae ◽  
Sung-Keun Rhee ◽  
Yong-Gyun Cho ◽  
Sung-Taik Lee
2006 ◽  
Vol 72 (6) ◽  
pp. 4274-4282 ◽  
Author(s):  
Amy V. Callaghan ◽  
Lisa M. Gieg ◽  
Kevin G. Kropp ◽  
Joseph M. Suflita ◽  
Lily Y. Young

ABSTRACT Recent studies have demonstrated that fumarate addition and carboxylation are two possible mechanisms of anaerobic alkane degradation. In the present study, we surveyed metabolites formed during growth on hexadecane by the sulfate-reducing isolates AK-01 and Hxd3 and by a mixed sulfate-reducing consortium. The cultures were incubated with either protonated or fully deuterated hexadecane; the sulfate-reducing consortium was also incubated with [1,2-13C2]hexadecane. All cultures were extracted, silylated, and analyzed by gas chromatography-mass spectrometry. We detected a suite of metabolites that support a fumarate addition mechanism for hexadecane degradation by AK-01, including methylpentadecylsuccinic acid, 4-methyloctadecanoic acid, 4-methyloctadec-2,3-enoic acid, 2-methylhexadecanoic acid, and tetradecanoic acid. By using d 34-hexadecane, mass spectral evidence strongly supporting a carbon skeleton rearrangement of the first intermediate, methylpentadecylsuccinic acid, was demonstrated for AK-01. Evidence indicating hexadecane carboxylation was not found in AK-01 extracts but was observed in Hxd3 extracts. In the mixed sulfate-reducing culture, however, metabolites consistent with both fumarate addition and carboxylation mechanisms of hexadecane degradation were detected, which demonstrates that multiple alkane degradation pathways can occur simultaneously within distinct anaerobic communities. Collectively, these findings underscore that fumarate addition and carboxylation are important alkane degradation mechanisms that may be widespread among phylogenetically and/or physiologically distinct microorganisms.


2020 ◽  
Vol 18 (2) ◽  
pp. 1189-1205
Author(s):  
Margarita Isabel Pérez-Díaz ◽  
Paola Zárate-Segura ◽  
Luis Antonio Bermeo-Fernández ◽  
Khemlal Nirmalkar ◽  
Fernando Bastida-González ◽  
...  

2009 ◽  
Vol 70 (3) ◽  
pp. 586-596 ◽  
Author(s):  
Susann Müller ◽  
Carsten Vogt ◽  
Mandy Laube ◽  
Hauke Harms ◽  
Sabine Kleinsteuber

2006 ◽  
Vol 138 (3) ◽  
pp. 589-593 ◽  
Author(s):  
Daoyong Zhang ◽  
Jianlong Wang ◽  
Xiangliang Pan

2013 ◽  
Vol 825 ◽  
pp. 540-543
Author(s):  
Mariana Moreira ◽  
Silvana de Queiroz Silva ◽  
Mônica Cristina Teixeira

The objective of this work was to identify one bacterial consortium adapted to the cultivation in the presence of trivalent arsenic (AsIII). Samples were cultured in flasks containing modified Postgate C liquid medium (selective for sulfate-reducing bacteria, SRB). Six different As concentrations were used: 0.5, 1.0, 2.0, 4.0, 8.0 and 16 mg l-1. The growth of sulfate reducing microorganisms was indirectly observed by the formation of an iron sulfide black precipitate and also by the Eh measures.100 ml aliquots of cultured media were centrifuged and stored at-20°C for DNA extraction by phenol/chloroform method. Universal primers 968F-GC 1392R (Bacteria domain) were used for 16S ribosomal DNA amplification. Microbial diversity was evaluated by denaturing gradient gel electrophoresis (DGGE). After DGGE analysis 7 different bands were selected, cut, sequenced and analyzed using the Ribosomal Database Project Release. Consortium microorganisms identified were: Pantoea agglomerans, Enterobacter sp, Citrobacter sp, Cupriavidusmetallidurans, Ralstonia sp, Burkholderia cepacia and Bacillus sp. Thus the microbial consortium here identified is a good candidate for bioremediation of arsenic contaminated areas and effluents.


2018 ◽  
Vol 40 (24) ◽  
pp. 3216-3226 ◽  
Author(s):  
Leandro Augusto Gouvêa de Godoi ◽  
Lucas Tadeu Fuess ◽  
Tiago Palladino Delforno ◽  
Eugenio Foresti ◽  
Marcia Helena Rissato Zamariolli Damianovic

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jasmine S. Berg ◽  
Arnaud Duverger ◽  
Laure Cordier ◽  
Christel Laberty-Robert ◽  
François Guyot ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document