Inhibitory Effect of 4-Cyanobenzaldehyde and 4-Cyanobenzoic Acid on Mushroom (Agaricus bisporus) Tyrosinase

2003 ◽  
Vol 22 (7/8) ◽  
pp. 607-612 ◽  
Author(s):  
Qiao Chen ◽  
Qing-Xi Chen ◽  
Ling Qiu ◽  
Kang-Kang Song ◽  
Huang Huang
2011 ◽  
Vol 233 (6) ◽  
pp. 1075-1079 ◽  
Author(s):  
Víctor Falguera ◽  
Oriol Miarnau ◽  
Jordi Pagán ◽  
Albert Ibarz

2013 ◽  
Vol 47 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Janusz Czapski

Activity of mushroom polyphenol oxidase (PPO) toward 6 substrates and inhibitory effect of cysteine, 2-mercaptoethanol, benzoic acid and sodium metabisulphite were determined. The o-diphenols which appeared to be the best substrates were: catechin, DOPA (L-3,4-dihydro-xyphenylalanine) and chlorogcnic acid. Affinity of PPO crude preparation substrates to enzyme, expressed as inverse value of Michaelis constant was lower then affinity of catechol. Inhibitory effect depended on specifity of inhibitors and their concentration. Electrophoretic patterns of PPO of mushrooms reveals slow and fast moving 4 isoforms when DOPA was used as a substrate, 2 bands for catechin and chlorogenic acid while only one band showed activity toward tyrosine and p-cresol.


Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.


2001 ◽  
Vol 120 (5) ◽  
pp. A176-A176
Author(s):  
P KOPPITZ ◽  
M STORR ◽  
D SAUR ◽  
M KURJAK ◽  
H ALLESCHER

2001 ◽  
Vol 120 (5) ◽  
pp. A655-A656
Author(s):  
H NAKAMURA ◽  
H YOSHIYAMA ◽  
H YANAI ◽  
M SHIRAL ◽  
T NAKAZAWA ◽  
...  

1958 ◽  
Vol 34 (2) ◽  
pp. 181-187 ◽  
Author(s):  
William O. Smith ◽  
Robert Hoke ◽  
Jerome Landy ◽  
Ranwel Caputto ◽  
Stewart Wolf

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
F Epifano ◽  
L Menghini ◽  
A Chiavaroli ◽  
G Orlando ◽  
VD La ◽  
...  
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