Molecular Cloning of a Zinc Finger Gene eZNF from a Human Inner Ear cDNA Library, and In Situ Expression Pattern of Its Mouse Homologue In Mouse Inner Ear

1998 ◽  
Vol 24 (2) ◽  
pp. 121-129 ◽  
Author(s):  
A.N.K. Jacob ◽  
N.A. Manjunath ◽  
Patricia Bray-Ward ◽  
Rajendra P. Kandpal
Keyword(s):  
Inner Ear ◽  
Molecular Cloning ◽  
Cdna Library ◽  
Expression Pattern ◽  
Zinc Finger ◽  
Zinc Finger Gene ◽  
Mouse Homologue ◽  
Human Inner Ear

Conserved segmental expression of Krox-20 in the vertebrate hindbrain and its relationship to lineage restriction

Development ◽  
1991 ◽  
Vol 113 (Supplement_2) ◽  
pp. 59-62 ◽  
Author(s):  
M. Angela Nieto ◽  
Leila C. Bradley ◽  
David G. Wilkinson
Keyword(s):  
Expression Pattern ◽  
Zinc Finger ◽  
Cell Lineage ◽  
Zinc Finger Gene ◽  
Early Stages ◽  
Cellular Events ◽  
Early Expression ◽  
Segmental Expression

The zinc-finger gene Krox-20 is expressed in two alternating segments, rhombomeres (r) 3 and 5, in the developing mouse hindbrain. This expression pattern is established prior to rhombomere formation in the mouse, but it is not known how the timing of expression relates to cellular events of segmentation, such as lineage restriction. We have cloned Krox-20 sequences from Xenopus and the chick and shown that its alternating expression pattern is conserved in these systems, suggesting that its role in hindbrain development is conserved. Analysis of the early stages of Krox-20 expression in the chick show that both domains of expression precede the restriction of cell lineage to specific rhombomeres, consistent with a role of this gene in early events of hindbrain segmentation. The finding that expression is not coincident with lineage restriction indicates that early expression may not reflect an irreversible commitment of cells to r3 and r5 and/or may be mosaic.

scholarly journals Antisense oligonucleotides from the stage-specific myeloid zinc finger gene MZF-1 inhibit granulopoiesis in vitro.

1991 ◽  
Vol 174 (5) ◽  
pp. 1097-1101 ◽  
Author(s):  
L Bavisotto ◽  
K Kaushansky ◽  
N Lin ◽  
R Hromas
Keyword(s):  
Zinc Finger ◽  
Antisense Oligonucleotides ◽  
Transcriptional Regulators ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Zinc Finger Gene ◽  
Myeloid Zinc Finger ◽  
Stimulating Factor

Zinc finger proteins are transcriptional regulators of other genes, often controlling developmental cascades of gene expression. A recently cloned zinc finger gene, MZF-1, was found to be preferentially expressed in myeloid cells. Using complementary radiolabeled MZF-1 RNA hybridized to human bone marrow smears in situ, it was discovered that the expression of MZF-1 is essentially limited to the myelocyte and metamyelocyte stages of granulopoiesis. Antisense but not sense oligonucleotides from MZF-1 significantly inhibited granulocyte colony-stimulating factor-driven granulocyte colony formation in vitro.

Insights into the Pathobiology of TMPRSS3-Related Hearing Loss and Implications for Cochlear Implant Patients with TMPRSS3 Mutations.

2021 ◽  
Author(s):  
Brady J. Tucker ◽  
Yuan-Siao Chen ◽  
Timothy J. Shin ◽  
Ernesto Cabrera ◽  
Kevin T. Booth ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Inner Ear ◽  
Expression Pattern ◽  
Autosomal Recessive ◽  
Poor Performance ◽  
Auditory Neurons ◽  
Spatial Expression Pattern ◽  
Human Cochlea ◽  
Syndromic Hearing Loss ◽  
Human Inner Ear

Abstract OBJECTIVES To review the audiological outcomes after cochlear implantation (CI) for TMPRSS3-associated autosomal recessive non-syndromic hearing loss (ARNSHL) and evaluate the spatial expression pattern of TMPRSS3 within the human cochlea. METHODS Review all published cases of CI in patients with TMPRSS3-associated ARNSHL to compare postoperative consonant-nucleus-consonant (CNC) word performance to published adult CI cohorts. Protein structural modeling of TMPRSS3 variants associated with post-lingual hearing loss. Determine TMPRSS3 expression pattern in human inner ear organoids and human cochlea. RESULTS Nine articles detailed 27 patients (30 total CI ears) with TMPRSS3-associated hearing loss treated with CI. Of these, 6 cases reported prelingual onset (< 2yo) and 24 cases reported post-lingual onset (≥2yo) of hearing loss. Subjectively, 85% of cases had a favorable outcome. Objectively, the postoperative mean (SD) post-operative CNC word score was not significantly different than other adults [66.2% (25.8%) correct vs. 50.1% (12.5%); F(1,6) = 1.97, P = 0.21]. In the TMPRSS3 cohort, poor performers (CNC < 30% correct) were significantly older than good performers [49 (± 13.3) years vs. 17.4 (± 18.4) years; P < 0.01] and all harbored the A138E variant. TMPRSS3 immunostaining is restricted to the otic epithelial cells and is not expressed within auditory neurons of human cochlea and human inner ear organoids. CONCLUSIONS Patients with TMPRSS3-related hearing loss exhibit similar postoperative performance to other adult CI patients. TMPRSS3 is not expressed in human auditory neurons and the duration of hearing loss prior to CI likely contributes to poor performance.

Molecular cloning and characterization of a rice blast-inducible RING-H2 type Zinc finger gene

DNA Sequence ◽  
2006 ◽  
Vol 17 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Xiang-Bing Meng ◽  
Wen-Sheng Zhao ◽  
Rui-Ming Lin ◽  
Min Wang ◽  
You-Liang Peng
Keyword(s):  
Molecular Cloning ◽  
Zinc Finger ◽  
Rice Blast ◽  
Zinc Finger Gene

Cloning and characterization of a novel human zinc finger gene, hKid3, from a C2H2-ZNF enriched human embryonic cDNA library

2004 ◽  
Vol 325 (4) ◽  
pp. 1145-1152 ◽  
Author(s):  
Li Gao ◽  
Chong Sun ◽  
Hong-Ling Qiu ◽  
Hui Liu ◽  
Huan-Jie Shao ◽  
...  
Keyword(s):  
Cdna Library ◽  
Zinc Finger ◽  
Zinc Finger Gene

scholarly journals Identification and disruption of an Arabidopsis zinc finger gene controlling seed germination

2000 ◽  
Vol 14 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Maura Papi ◽  
Sabrina Sabatini ◽  
David Bouchez ◽  
Christine Camilleri ◽  
Paolo Costantino ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Zinc Finger ◽  
Segregation Analysis ◽  
Reverse Genetics ◽  
Vascular Tissue ◽  
Zinc Finger Transcription Factor ◽  
Zinc Finger Gene ◽  
Dna Insertion

We describe here the Arabidopsis gene DAG1, encoding a zinc finger transcription factor of the Dof family, and show that it is involved in the control of seed germination. By a reverse genetics approach, we isolated an Arabidopsis mutant line with one T-DNA insertion in DAG1. Seeds from homozygous knockoutdag1-1 plants do not develop dormancy and germinate also in the absence of light. Segregation analysis indicates that the effect of the mutation is maternal. Accordingly, in situ mRNA hybridizations reveal expression of DAG1 in the vascular tissue of the flower and maturing fruit but not in the seed.

1227 Molecular cloning of a novel zinc finger gene, gcz, expressed in the granule cell layer

1996 ◽  
Vol 25 ◽  
pp. S135
Author(s):  
Kazumasa Matsumoto ◽  
Nobuko Ishii ◽  
Akio Wanaka ◽  
Masaya Tohyama
Keyword(s):  
Molecular Cloning ◽  
Zinc Finger ◽  
Granule Cell ◽  
Cell Layer ◽  
Granule Cell Layer ◽  
Zinc Finger Gene
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