Somatic Embryogenesis from 20 Open-Pollinated Families of Portuguese Plus Trees of Maritime Pine

2004 ◽  
Vol 76 (2) ◽  
pp. 121-130 ◽  
Author(s):  
Célia Miguel ◽  
Sónia Gonçalves ◽  
Susana Tereso ◽  
Liliana Marum ◽  
João Maroco ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Maritime Pine

scholarly journals Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

2009 ◽  
Vol 28 (4) ◽  
pp. 673-682 ◽  
Author(s):  
Liliana Marum ◽  
Margarida Rocheta ◽  
João Maroco ◽  
M. Margarida Oliveira ◽  
Célia Miguel
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Pinus Pinaster ◽  
Maritime Pine ◽  
Ssr Loci

Somatic embryogenesis from different tissues of Spanish populations of maritime pine

2012 ◽  
Vol 111 (3) ◽  
pp. 373-383 ◽  
Author(s):  
A. Humánez ◽  
M. Blasco ◽  
C. Brisa ◽  
J. Segura ◽  
I. Arrillaga
Keyword(s):  
Somatic Embryogenesis ◽  
Maritime Pine ◽  
Spanish Populations ◽  
Different Tissues

scholarly journals New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

2019 ◽  
Vol 10 ◽  
Author(s):  
Isabel Arrillaga ◽  
Marian Morcillo ◽  
Israel Zanón ◽  
Francisco Lario ◽  
Juan Segura ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Maritime Pine ◽  
New Approaches

scholarly journals Priming Maritime Pine Megagametophytes during Somatic Embryogenesis Improved Plant Adaptation to Heat Stress

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 446
Author(s):  
María Amparo Pérez-Oliver ◽  
Juan Gregorio Haro ◽  
Iva Pavlović ◽  
Ondřej Novák ◽  
Juan Segura ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Heat Stress ◽  
Global Climate ◽  
Soluble Sugars ◽  
Heat Exposure ◽  
Forest Tree ◽  
Maritime Pine ◽  
Induction Phase ◽  
Putative Transcription Factor

In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregulation, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ɸPSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

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