PMN responses in chronic periodontal disease: evaluation by gingival crevicular fluid enzymes and elastase-alpha-1-proteinase inhibitor complex

2002 ◽  
Vol 29 (6) ◽  
pp. 563-572 ◽  
Author(s):  
Rainer Buchmann ◽  
Andrej Hasilik ◽  
Martha E. Nunn ◽  
Thomas E. Van Dyke ◽  
Dieter E. Lange
Keyword(s):  
Periodontal Disease ◽  
Proteinase Inhibitor ◽  
Gingival Crevicular Fluid ◽  
Inhibitor Complex ◽  
Chronic Periodontal Disease ◽  
Crevicular Fluid

Amplified Crevicular Leukocyte Activity in Aggressive Periodontal Disease

2002 ◽  
Vol 81 (10) ◽  
pp. 716-721 ◽  
Author(s):  
R. Buchmann ◽  
A. Hasilik ◽  
T.E. Van Dyke ◽  
D.E. Lange
Keyword(s):  
Periodontal Disease ◽  
Surgical Therapy ◽  
Proteinase Inhibitor ◽  
Gingival Crevicular Fluid ◽  
Aggressive Periodontitis ◽  
Local Inflammatory Response ◽  
Inhibitor Complex ◽  
Leukocyte Activity ◽  
Crevicular Fluid ◽  
Non Surgical Treatment

Exaggerated neutrophil responses are a critical component in the pathogenesis of periodontal disease. We investigated whether leukocyte activity in aggressive periodontitis (AP) is increased compared with that in chronic periodontitis (CP) by gingival crevicular fluid (GCF) analysis of myeloperoxidase (MPO), beta-N-acetyl-hexosaminidase (beta-NAH), cathepsin D (CD), and elastase-alpha-1-proteinase inhibitor complex (alpha-1-EPI) before and 6 months after therapy. Initial AP neutrophil responses were significantly amplified compared with those in CP (MPO, 3.2-fold; beta-NAH, 37.5-fold; CD, 2.2-fold; alpha-1-EPI, 1.4-fold; p < 0.05). Surgical therapy resulted in a significant reduction of GCF markers compared with non-surgical treatment. However, the changes in clinical parameters were not different between AP and CP (P > 0.05). Analysis of the results suggests that the local inflammatory response in AP is characterized by increased release of inflammatory mediators of neutrophil origin into the GCF. Analysis of the data further suggests that surgical therapy is a more predictable method for removal of the pro-inflammatory etiology.

RP-HPLC assay of doxycycline in human saliva and gingival crevicular fluid in patients with chronic periodontal disease

2013 ◽  
Vol 78-79 ◽  
pp. 170-175 ◽  
Author(s):  
Marko S. Denić ◽  
Slavica M. Sunarić ◽  
Ljiljana G. Kesić ◽  
Ivan Z. Minić ◽  
Radmila R. Obradović ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Human Saliva ◽  
Hplc Assay ◽  
Rp Hplc ◽  
Chronic Periodontal Disease ◽  
Crevicular Fluid

scholarly journals Levels of prostaglandin E2 (PGE2) in gingival crevicular fluid from smokers and non-smokers with gingivitis and chronic periodontal disease

2015 ◽  
Vol 7 (5) ◽  
pp. 54-59 ◽  
Author(s):  
Alessandra da Cruz Galhardo Camargo Gabriela ◽  
Pereira dos Santos Marcelo ◽  
Linhares Coutinho Silva Natalia ◽  
Luisa Palhares de Miranda Ana ◽  
Luiz Mendonca Tributino Jorge
Keyword(s):  
Periodontal Disease ◽  
Prostaglandin E2 ◽  
Gingival Crevicular Fluid ◽  
Chronic Periodontal Disease ◽  
Crevicular Fluid

scholarly journals A pilot study of active enzyme levels in gingival crevicular fluid of patients with chronic periodontal disease

2016 ◽  
Vol 43 (8) ◽  
pp. 629-636 ◽  
Author(s):  
Sarhang S. Gul ◽  
Charles W. I. Douglas ◽  
Gareth S. Griffiths ◽  
Andrew Rawlinson
Keyword(s):  
Pilot Study ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Active Enzyme ◽  
Chronic Periodontal Disease ◽  
Crevicular Fluid

Salivary Diagnosis - Clinical Uses in Assessing Oral Inflammation

2017 ◽  
Vol 68 (6) ◽  
pp. 1201-1204 ◽  
Author(s):  
Iulia Ioana Stanescu ◽  
Alexandra Totan ◽  
Florentina Rus ◽  
Daniela Miricescu ◽  
Brandusa Mocanu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Oxidative Stress Marker ◽  
Clinical Settings ◽  
Tissue Destruction ◽  
Whole Saliva ◽  
Oral Inflammation ◽  
Positive Correlations ◽  
Crevicular Fluid

The past decades demonstrated that saliva and its components represent a remarkable diagnosis fluid with valuable clinical uses for both oral and systemic diseases. At the same time it is well established that oxidative stress is involved in a wide number of pathologies, including periodontitis. The specific aim of the present study which included 50 subjects is to determine if saliva can be used in clinical settings to correlate oxidative stress and tissue destruction markers with the severity of periodontal disease. An important oxidative stress marker - 8-hydroxydesoxyguanosine (8-OHdG) and a collagen degradation marker - beta-crosslaps (b-CTX) were quantified in both saliva and gingival crevicular fluid (GCF) using ELISA kits and were found to be significantly increased in the chronic periodontitis group when compared to respective controls (p[0.05). At the same time positive correlations were observed between whole saliva and gingival crevicular fluid (p[0.05). Significant correlations were also determined between GCF and salivary markers and clinical parameters of periodontal disease. Present results demonstrate that saliva and its components can successfully be used in clinical settings and represents a reliable tool for assessing periodontal disease severity.

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