RP-HPLC assay of doxycycline in human saliva and gingival crevicular fluid in patients with chronic periodontal disease

The past decades demonstrated that saliva and its components represent a remarkable diagnosis fluid with valuable clinical uses for both oral and systemic diseases. At the same time it is well established that oxidative stress is involved in a wide number of pathologies, including periodontitis. The specific aim of the present study which included 50 subjects is to determine if saliva can be used in clinical settings to correlate oxidative stress and tissue destruction markers with the severity of periodontal disease. An important oxidative stress marker - 8-hydroxydesoxyguanosine (8-OHdG) and a collagen degradation marker - beta-crosslaps (b-CTX) were quantified in both saliva and gingival crevicular fluid (GCF) using ELISA kits and were found to be significantly increased in the chronic periodontitis group when compared to respective controls (p[0.05). At the same time positive correlations were observed between whole saliva and gingival crevicular fluid (p[0.05). Significant correlations were also determined between GCF and salivary markers and clinical parameters of periodontal disease. Present results demonstrate that saliva and its components can successfully be used in clinical settings and represents a reliable tool for assessing periodontal disease severity.

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Total Protein Concentration of Gingival Crevicular Fluid as an Indicator of the Severity of Periodontal Disease.

Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) ◽

10.2329/perio.33.929 ◽

1991 ◽

Vol 33 (4) ◽

pp. 929-935

Author(s):

Kazushi KUNIMATSU ◽

Hidetaka TANAKA ◽

Naoko MINE ◽

Kousuke MURAKAMI ◽

Ihachi KATO

Keyword(s):

Periodontal Disease ◽

Total Protein ◽

Protein Concentration ◽

Gingival Crevicular Fluid ◽

Total Protein Concentration ◽

Crevicular Fluid

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NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

Mediators of Inflammation ◽

10.1155/2021/6917919 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Petra Surlin ◽

Luminita Lazar ◽

Cerasella Sincar ◽

Dorin Nicolae Gheorghe ◽

Dora Maria Popescu ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Periodontal Disease ◽

Chronic Hepatitis C ◽

Inflammatory Reaction ◽

Nlrp3 Inflammasome ◽

Gingival Crevicular Fluid ◽

Periodontal Status ◽

Local Inflammatory Reaction ◽

Crevicular Fluid

The study is aimed at assessing the impact that periodontal disease and chronic hepatitis C could have on gingival crevicular fluid levels of the NLRP3 inflammasome, caspase-1 (CASP-1), and interleukin-18 (IL-18) and at evaluating whether the increased local inflammatory reaction with clinical periodontal consequences is correlated to their upregulation. Patients were divided into four groups, according to their periodontal status and previously diagnosed hepatitis C, as follows: (i) CHC group, chronic hepatitis C patients; (ii) P group, periodontal disease patients, systemically healthy; (iii) CHC + P group, patients suffering from both conditions; and (iv) H group, systemically and periodontally healthy controls. Gingival crevicular samples were collected for quantitative analysis of the NLRP3 inflammasome, CASP-1, and IL-18. CHC + P patients expressed the worse periodontal status and the highest NLRP3, CASP-1, and IL-18 levels, the difference being statistically significant ( p < 0.05 ). The P group patients also expressed significantly more elevated NLRP3, CASP-1, and IL-18 levels, as compared to nonperiodontal patients (CHC and H groups). Chronic hepatitis C and periodontal disease could have a significant influence on the upregulation of NLRP3 inflammasome and its components, possibly contributing to an increased local inflammatory reaction and clinical periodontal consequences.

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