‘Solubilization’ of Carcinogens with Nucleic Acid Solutions. The Interaction of p-Dimethylaminoazobenzene with Ribonucleic Acids

Nature ◽  
1964 ◽  
Vol 202 (4936) ◽  
pp. 1010-1010 ◽  
Author(s):  
CLAUDE MARMASSE
FEBS Letters ◽  
1997 ◽  
Vol 405 (2) ◽  
pp. 141-144 ◽  
Author(s):  
Iliana I Timcheva ◽  
Vera A Maximova ◽  
Todor G Deligeorgiev ◽  
Nikolai I Gadjev ◽  
Ram W Sabnis ◽  
...  

Biopolymers ◽  
1966 ◽  
Vol 4 (7) ◽  
pp. 723-735
Author(s):  
Carl R. Merril ◽  
Marvin B. Shapiro ◽  
Dan F. Bradley ◽  
James E. Mosimann ◽  
Jay E. Vinton

2009 ◽  
Vol 37 (2) ◽  
pp. 466-470 ◽  
Author(s):  
Rupak Doshi ◽  
Philip J.R. Day ◽  
Nicola Tirelli

Nucleic acids are routinely and readily analysed using the A260/A280 ratio, although this method is known to be prone to erroneous results owing to contaminants in solution that absorb at similar wavelengths. The aim of the present review, while highlighting the problems and alternatives of using UV spectrophotometry for nucleic acid measurements, is to bring forth an observational result from our recent studies, namely that DO (dissolved oxygen) and nitrogen can alter the A260 of aqueous DNA solutions. Our finding is of importance because DO is highly variable between protocols and storage conditions of DNA preparations. The physicochemical nature of the oxygen–DNA interactions is briefly discussed.


1953 ◽  
Vol 11 (3) ◽  
pp. 277-280 ◽  
Author(s):  
B. E. Conway ◽  
J. A. V. Butler
Keyword(s):  

1971 ◽  
Vol 26 (3) ◽  
pp. 235-243 ◽  
Author(s):  
W. Hennig ◽  
W. Kunz ◽  
B. Schnieders ◽  
K. Williams

Ribonucleic acids labeled with 14C- or 3H-orotic acids, respectively were extracted from aliquots of mouse liver homogenates according to the cold-phenol-method of Kirby or the method of Georgiev employing different temperature steps.This comparative analysis showed that only ca. 50% of the total labeled RNA was extracted by the Kirby method. Especially the high molecular D-RNA and R-RNA were not sufficiently extracted, as was demonstrated by hydroxyapatite- and methylalbumin-Kieselgur column chromatography.Reextraction of the tissue at 65 °C first extracted by the Kirby method resulted in a recovery of most of the missing nucleic acid species.Therefore, results obtained by the Kirby and Georgiev methods will be different in regard to the nucleic acid composition. These findings could explain contradictary results about this problem in literature.


2004 ◽  
Vol 08 (15) ◽  
pp. 833-835

Zenosis Launches Two New Titles for Professionals Involved in Clinical Trials. Nucleic Acid Solutions Facilitator: Proligo Singapore.


1974 ◽  
Vol 185 (1080) ◽  
pp. 343-356 ◽  

Calcium phosphate co-precipitates half its mass of ribonucleic acids made from yeast, tobacco leaf and tobacco mosaic virus when the precipitate is formed in their presence. It also precipitates deoxyribonucleic acid. Degraded nucleic acids are less completely precipitated. Less nucleic acid is adsorbed by preformed calcium phosphate. In suitable circumstances nucleic acid is fixed by leaf fibre. Autolytic changes in leaf fibre, and in calcium-chelators in leaf extracts, explain many differences in the amount of nucleic acid present in extracts made from tobacco leaves in different ways.


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