Circular Dichroism of Long Wavelength Forms of Chlorophyll a

Nature ◽  
1969 ◽  
Vol 224 (5224) ◽  
pp. 1108-1110 ◽  
Author(s):  
GWENDOLYN M. SHERMAN
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Long Wavelength ◽  
Circular Dichroïsm

Long-wavelength circular dichroism of dithiomides

1988 ◽  
pp. 853-854 ◽  
Author(s):  
Tadeusz Połoński
Keyword(s):  
Circular Dichroism ◽  
Long Wavelength ◽  
Circular Dichroïsm

The state of detergent solubilised light-harvesting chlorophyll-a/b protein complex as monitored by picosecond time-resolved fluorescence and circular dichroism

1987 ◽  
Vol 893 (2) ◽  
pp. 349-364 ◽  
Author(s):  
Jonathan P. Ide ◽  
David R. Klug ◽  
Werner Kühlbrandt ◽  
Linda B. Giorgi ◽  
George Porter
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Light Harvesting ◽  
The State ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Circular Dichroïsm

Strong circular dichroism of chlorophyll a in 50 : 50 ethanol–water solution

1980 ◽  
pp. 1056-1056 ◽  
Author(s):  
Vlastimil Fidler ◽  
Alexander D. Osborne
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Water Solution ◽  
Circular Dichroïsm

Circular Dichroism and Ultraviolet Absorbance of Calf Thymus DNA in Presence of CH3HgOH

1979 ◽  
Vol 34 (3-4) ◽  
pp. 259-265 ◽  
Author(s):  
Michael S. Clegg ◽  
Dieter W. Gruenwedel
Keyword(s):  
Circular Dichroism ◽  
Absorption Band ◽  
Calf Thymus Dna ◽  
Absorbance Spectrum ◽  
Absorption Bands ◽  
Long Wavelength ◽  
Ultraviolet Absorbance ◽  
Rotational Strength ◽  
Calf Thymus ◽  
Circular Dichroïsm

Abstract The changes that one observes upon the addition of CH3HgOH in the circular dichroism spec­trum and ultraviolet absorbance spectrum of native calf thymus DNA, dissolved in buffered (pH 6.8) solutions of Na2SO4 at pNa 2.0, 1.5, 1.0, and 0.0, respectively (pNa = - log [Na+]), are shown to be due to denaturation brought about by the organomercurial interacting with the base moieties of the polymer. The changes are characterized by an extensive shift of both spectra to longer wavelengths, by a decrease of the rotational strength of the long-wavelength positive dichroic absorption band, and by an increase in the UV absorbance at λmax . Both the hyperchromicity Hλ of calf thymus DNA and the normalized decrease of the rotational strength of its long-wavelength positive dichromic band, Ω , display cooperativity when plotted against the methylmercury concentration pM (pM = - log [CH3HgOH]added) at a given salt strength. Rotational strength data, evaluated by integration of the area under the positive and negative dichroic absorption bands, have been tabulated for selected values of pNa and pM. They are compared with data available from the literature. In absence of CH3HgOH, and with varying salt strength, native calf thymus DNA exhibits alterations in the long-wavelength positive dichroic absorption band that are interpreted as representing B → C transitions in agreement with currently held views regarding their origin. Similar salt-induced alterations have been noted in the case of denatured DNA; their meaning in terms of DNA geometry remains unclear at this point in time.

scholarly journals Evidence that circularly dichroic chlorophyll forms a-682 and a-710 are oriented at right angles to the thylakoid membranes of whole chloroplasts, and that the circular dichroism is light-dependent

1975 ◽  
Vol 148 (3) ◽  
pp. 487-497 ◽  
Author(s):  
R P Gregory
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
The Other ◽  
Light Path ◽  
Preferred Direction ◽  
Polarization Of Fluorescence ◽  
Chloroplast Orientation ◽  
Circular Dichroïsm ◽  
Chlorophyll Forms ◽  
Exciton Type

Chloroplasts from the pea (Pisum sativum L.) suspended in iso-osmotic buffered medium were oriented by flow in specially constructed cuvettes and examined for circular dichroism (c.d.). In one cuvette the flow was transverse to the direction of the light-path, but the other cuvette was designed so that flow and the light-path were coaxial. The induced orientation is such that the chloroplasts appear to move edgewise. C.d. was maximum when the light-path lay in the plane of the chloroplast disc. The intense c.d. of intact chloroplasts ascribed by Gregory & Raps [Biochem. J. (1974) 142, 193-201] to bulk chlorophyll a was found to contain two components, one the split-exciton type centred at 682nm and the other a simple maximum at 700-710 nm. The chlorophyll a-710 form was distinguished by its greater dependence on chloroplast orientation. The preferred direction of the transition moment in both chlorophyll forms was at right angles to the plane of the chloroplast, that is, at right angles to the plane of the thylakoids. This is in conflict with several reports based on polarization of fluorescence. It is suggested that the present effect is due to thylakoid-thylakoid interaction. Evidence for this is the reversible diminution in the c.d. signal caused by illumination in the presence of electron-transport reagents. It is argued that the c.d. is an indicator of chlorophyll movement, or changes in the thylakoid-thylakoid distance, possibly related to ion movement, affecting energy transfer between photosynthetic units.

scholarly journals Organization of Chlorophyll a in the Light-Harvesting Chlorophyll a/b Protein Complex as Shown by Circular Dichroism

1983 ◽  
Vol 73 (1) ◽  
pp. 16-19 ◽  
Author(s):  
Ágnes Faludi-Dániel ◽  
László A. Mustárdy
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Light Harvesting ◽  
Circular Dichroïsm

Reversible changes in macroorganization of the light-harvesting chlorophyll a/b pigment-protein complex detected by circular dichroism

Biochemistry ◽  
1988 ◽  
Vol 27 (7) ◽  
pp. 2430-2434 ◽  
Author(s):  
Gyozo Garab ◽  
Richard C. Leegood ◽  
David A. Walker ◽  
John C. Sutherland ◽  
Geoffrey Hind
Keyword(s):  
Circular Dichroism ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Light Harvesting ◽  
Pigment Protein Complex ◽  
Circular Dichroïsm
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