Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation

Nature ◽  
1993 ◽  
Vol 366 (6450) ◽  
pp. 44-48 ◽  
Author(s):  
Brent Bailey ◽  
Daniel L. Farkas ◽  
D. Lansing Taylor ◽  
Frederick Lanni
Keyword(s):  
Fluorescence Microscopy ◽  
Standing Wave ◽  
Wave Excitation ◽  
Axial Resolution

Enhanced axial resolution of the cytoskeleton in fluorescence microscopy by standing-wave excitation

1993 ◽  
Vol 51 ◽  
pp. 88-89
Author(s):  
Frederick Lanni ◽  
Brent Bailey ◽  
Daniel L. Farkas ◽  
D. Lansing Taylor
Keyword(s):  
Fluorescence Microscopy ◽  
Standing Wave ◽  
Focal Plane ◽  
Information Transfer ◽  
High Speed ◽  
Depth Resolution ◽  
Depth Of Field ◽  
Imaging Method ◽  
Axial Resolution ◽  
Direct Imaging

When the depth-of-field of a microscope is less than the axial dimension of the specimen, 3d information can be derived from a set of images recorded as the specimen is stepped through the object focal plane of the microscope. This procedure, known as optical sectioning microscopy (OSM), is the same in direct imaging and confocal scanning. For both of these cases in fluorescence microscopy, axial (depth) resolution is more limited than transverse resolution, for fundamental reasons. Our research aim has been to enhance axial resolution in fluorescence OSM (FOSM) while retaining the high-speed information transfer characteristics of direct imaging that are necessary for 3d studies of living cells in culture.Standing-wave fluorescence microscopy (SWFM) is a direct imaging method in which the object is illuminated by a three-dimensional field of planar interference fringes (standing waves) oriented parallel to the focal plane of the microscope. This field is produced in the specimen by crossing two coherent, collimated, s-polarized beams of equal amplitude directed through the specimen at complementary angles (θ, π -θ) relative to the axis of the microscope.

Standing-wave excitation for fluorescence microscopy

1994 ◽  
Vol 4 (7) ◽  
pp. 262-265 ◽  
Author(s):  
Frederick Lanni ◽  
Brent Bailey
Keyword(s):  
Fluorescence Microscopy ◽  
Standing Wave ◽  
Wave Excitation

scholarly journals Axial Resolution Enhancement by 4Pi Confocal Fluorescence Microscopy with Two-Photon Excitation

2007 ◽  
Vol 33 (5-6) ◽  
pp. 433-443 ◽  
Author(s):  
Sylvia Glaschick ◽  
Carlheinz Röcker ◽  
Karen Deuschle ◽  
Jörg Wiedenmann ◽  
Franz Oswald ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Resolution Enhancement ◽  
Confocal Fluorescence Microscopy ◽  
Axial Resolution ◽  
Two Photon ◽  
Photon Excitation ◽  
Confocal Fluorescence ◽  
Two Photon Excitation

Hard x-ray photoemission spectroscopy on the trilayer system MgO/Au/Fe using standing-wave excitation

2013 ◽  
Vol 46 (37) ◽  
pp. 375001 ◽  
Author(s):  
S Döring ◽  
F Schönbohm ◽  
U Berges ◽  
D E Bürgler ◽  
C M Schneider ◽  
...  
Keyword(s):  
Standing Wave ◽  
Photoemission Spectroscopy ◽  
Wave Excitation ◽  
X Ray

Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization

2005 ◽  
Vol 246 (4-6) ◽  
pp. 313-321 ◽  
Author(s):  
M.T. Caballero ◽  
P. Andrés ◽  
A. Pons ◽  
J. Lancis ◽  
M. Martínez-Corral
Keyword(s):  
Fluorescence Microscopy ◽  
Axial Resolution

scholarly journals Hard x-ray photoemission using standing-wave excitation applied to the MgO/Fe interface

2011 ◽  
Vol 83 (16) ◽  
Author(s):  
Sven Döring ◽  
Frank Schönbohm ◽  
Ulf Berges ◽  
Reinert Schreiber ◽  
Daniel E. Bürgler ◽  
...  
Keyword(s):  
Standing Wave ◽  
Wave Excitation ◽  
X Ray

Visualization of the Microtubules of Glutaraldehyde-Fixed Cells by Reflection-Enhanced Backscatter Confocal Microscopy

2005 ◽  
Vol 12 (2) ◽  
pp. 113-123
Author(s):  
Charles H. Keith ◽  
Mark A. Farmer
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Standing Wave ◽  
Reflection Mode ◽  
Intact Cells ◽  
Fluorescence Techniques ◽  
Number Of Factors

Performing reflection-mode (backscatter-mode) confocal microscopy on cells growing on reflective substrates gives images that have improved contrast and are more easily interpreted than standard reflection-mode confocal micrographs (Keith et al., 1998). However, a number of factors degrade the quality of images taken with the highest-resolution microscope objectives in this technique. We here describe modifications to reflection-enhanced backscatter confocal microscopy that (partially) overcome these factors. With these modifications of the technique, it is possible to visualize structures the size—and refractility—of individual microtubules in intact cells. Additionally, we demonstrate that this technique, in common with fluorescence techniques such as standing wave widefield fluorescence microscopy and 4-Pi confocal microscopy, offers improved resolution in the Z-direction.

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