Improvement in Lactose Tolerance in Hypolactasic Subjects Consuming Ice Creams with High or Low Concentrations of Bifidobacterium bifidum 900791

Although Bifidobacterium bifidum expresses lactase activity, no clinical trials have determined its impact on lactose-intolerant subjects. This study evaluated whether acute and chronic ingestion of ice creams containing B. bifidum 900791 at high (107 CFU/g) or low (105 CFU/g) concentrations improved lactose tolerance in hypolactasic subjects. Fifty subjects were selected based on a positive lactose (20 g) hydrogen breath test (HBT0) and the presence of digestive symptoms. The recruited subjects were required to perform breath tests after the acute ingestion of: (1) ice cream containing 20 g of lactose without a probiotic (HBT1); (2) the same ice cream, accompanied by a lactase tablet (HBT2); (3) the same ice cream containing the low or high dose of probiotic (HBT3-LD and HBT3-HD); and (4) after the chronic consumption of the ice cream without (placebo) or with the low concentration of probiotic for 1 month (HBT4). Significant decreases in H2 excretion during HBT2 and HBT3-HD as well as digestive symptoms during HBT2, HBT3-HD and HBT3-LD were observed compared to HBT0 and HBT1, while the orocecal transit time increased. Chronic consumption of the probiotic ice cream did not enhance lactose tolerance compared to the placebo. These results suggest that the acute ingestion of ice cream containing high or low concentrations of B. bifidum 900791 improves lactose tolerance in hypolactasic subjects.

Lactose tolerance test as an alternative to hydrogen breath test in the study of lactose malabsorption

ObjectivesLactose malabsorption is generally assessed by hydrogen breath testing (HBT). However, this test is not recommended in patients with high baseline hydrogen concentrations (H2B). In addition, breath testing is not recommended in the current situation created by the COVID-19 pandemic, due to the potential infectiveness of the samples. The objective is to assess concordance between HBT and lactose tolerance test (LTT) depending on H2B concentrations.MethodsA total of 430 patients (40 years, Q1–Q3 = 28–54 years; 66.7% women) suspected of lactose malabsorption were included in the study. Breath and heparinized blood samples were collected at baseline and sequentially after the intake of 50 g of lactose, to measure hydrogen in breath and glycemia in blood, respectively.ResultsH2B was <10 ppm in 69.5% of subjects; 10–20 ppm in 14.7%; and >20 ppm in 15.8% of subjects. In patients with H2B <20 ppm, concordance between HBT and LTT was moderate and consistently improved when the cut-off in LTT was set at 15 mg/dL. The increase in hydrogen and glucose correlated negatively (r=−0.389; p<0.05). The increase in glycemia during LTT was not influenced by H2B levels obtained in HBT.ConclusionsLTT emerges as an alternative to HBT to assess lactose malabsorption in the presence of high H2B levels or when breath testing is not recommended by the circumstances. The best concordance was obtained when the cut-off for LTT was set at 15 mg/dL.

Lactose intolerance and its association with the exogamy degree among the population of eastern Ukraine

Aim. The aim of our study was to evaluate the relationship between exogamy degree and lactose assimialation among the population of Eastern Ukraine in the Kharkiv region. Methods. The analysis included information on consumption and assimilation of milk and dairy products by 733 people aged from 14 to 79 years were. Statistical analysis included Shapiro-Wilk test, Pearson fitting (χ2) criterion and Spearman’s correlation analysis. Results. The lactose tolerance (LT) phenotype was found in 69.5%, whereas lactose intolerance (LI) – in 8.8% (from 3.5% to 24.1% in different age groups). In the age group 30-39 years: LI frequency was 24.1% being 2.5-6 times higher than in other groups. The exogamy degree of parents (EDP) showed in the group aged from 30 to 39 years the first EDP was 15.4%, being 1.4-2.5 times less (p = 0,05) than in other groups. Among people with bone marrow disorders the proportion of people with the first and second EDP was 60.9%, being 2.1 to 6.0 times higher than in other groups. Reduced EDP or increased inbreeding level causes the similarity of the chromosomes and affects the density of contact points between them during meiosis, level of recombination can be increased and associated with multifactorial traits. Conclusions. The results can be explained by the effect of the inbreeding level reduction in the presence of a high level of exogamy of parents, which causes high genetic diversity. Keywords: lactose intolerance, lactose tolerance, exogamy degree of parents, single nucleotide polymorphisms 13910C-T and 22018G-A, gene МСМ6.

Study of prevalence for lactose malabsorption in malnutrition

Background: Milk is the major food in the diet of the infant mammal and consequently has always attracted considerable interest from the physician, the industrialist and the mother. The concentration of lactose in milk varies from species to species. Main objective is to study the prevalence of malabsorption in malnutrition.Methods: Sixty-seven children ranged between ages of 6 months to 3 years, of both sex and varying grades of malnutrition, were studied. Study conducted between July 1979-December 1979. All children were grouped into 4 grades of malnutrition according to Indian Academy of Pediatrics recommendation.Results: Out of 67 cases, 50 were followed up. Severe malnutrition of grades III and IV (less than 60 percent of expected body weight) comprises 58.2 percent (39 out of 67) in first seen group, and 52 percent (26 out of 50) in follow up group. 59.7 percent Males and 40.3 percent females in first seen cases. 58 percent males and 42 percent females in follow up cases. 24 cases (53.33 percent) of lactose malabsorbers were males and 21 cases (46.67 percent) were females.Conclusions: Lactose malabsorption is common in protein-energy malnutrition. This can be determined by simple ward tests and abnormal lactose tolerance curve after oral lactose load. Lactose malabsorption increases with the severity of malnutrition. Malnutrition leads to lactose malabsorption and lactose malabsorption is an important contributory factor in production of malnutrition.

Got Milk? How Freedoms Evolved From Dairying Climates

The roots and routes of cultural evolution are still a mystery. Here, we aim to lift a corner of that veil by illuminating the deep origins of encultured freedoms, which evolved through centuries-long processes of learning to pursue and transmit values and practices oriented toward autonomous individual choice. Analyzing a multitude of data sources, we unravel for 108 Old World countries a sequence of cultural evolution reaching from (a) ancient climates suitable for dairy farming to (b) lactose tolerance at the eve of the colonial era to (c) resources that empowered people in the early industrial era to (d) encultured freedoms today. Historically, lactose tolerance peaks under two contrasting conditions: cold winters and cool summers with steady rain versus hot summers and warm winters with extensive dry periods (Study 1). However, only the cold/wet variant of these two conditions links lactose tolerance at the eve of the colonial era to empowering resources in early industrial times, and to encultured freedoms today (Study 2). We interpret these findings as a form of gene-culture coevolution within a novel thermo-hydraulic theory of freedoms.