scholarly journals Triple-Tracer Autoradiography of Cerebral Blood Flow, Glucose Utilization, and Protein Synthesis in Rat Brain

1986 ◽  
Vol 6 (1) ◽  
pp. 59-70 ◽  
Author(s):  
G. Mies ◽  
W. Bodsch ◽  
W. Paschen ◽  
K.-A. Hossmann
Keyword(s):  
Blood Flow ◽  
Protein Synthesis ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Processing System ◽  
Glucose Consumption ◽  
Utilization Rate ◽  
Digital Subtraction ◽  
Autoradiographic Technique ◽  
Simultaneous Assessment

A triple-tracer autoradiographic technique is described that permits the simultaneous measurement of cerebral blood flow, glucose consumption, and protein synthesis using 131I-iodoantipyrine (131I-IAP), [14C]deoxyclusively from 14C disintegrations without contamination by either 131I or 3H and that represented regional glucose utilization. Brain sections were then wash-incubated for 12 h to remove [14C]DG, [14C]DG-6-phosphate, and free 3H-amino acids from the tissue, and exposed to 3H-sensitive LKB Ultro-film for 2 weeks for autoradiography of 3H-amino acid incorporation into proteins. 14C radioactivity remaining in the tissue section after wash-incubation was determined by exposing sections again for 2 weeks to Kodak NMB film; the resulting contribution to the blackening of 3H-autoradiograms was corrected for by means of digital subtraction using an image-processing system. The triple-tracer autoradiographic technique was validated in rats under various physiological and pathophysiological conditions. In intact animals extinction correction was necessary only for 3H-autoradiograms. Under pathophysiological conditions, however, significant contamination of 131I by 14C occurred in regions with low blood flow and increased glucose utilization rate; this also required correction by digital subtraction. The interpretation of triple-tracer autoradiographic results is limited by the same restrictions as single-tracer autoradiography, but the simultaneous assessment of the three parameters considerably facilitates the interpretation of the flow/metabolic relationship, particularly under pathological conditions.

scholarly journals Local Cerebral Blood Flow and Glucose Consumption of Rats with Experimental Gliomas

1982 ◽  
Vol 2 (1) ◽  
pp. 25-32 ◽  
Author(s):  
K.-A. Hossmann ◽  
I. Niebuhr ◽  
M. Tamura
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Brain Tumors ◽  
Brain Tissue ◽  
Glucose Utilization ◽  
Cell Clone ◽  
Tumor Development ◽  
Glucose Consumption ◽  
Contralateral Side ◽  
Opposite Hemisphere

Experimental brain tumors were produced in rats by intracerebral implantation of a neoplastic glial cell clone. Within 2–6 weeks, spherical brain tumors developed at the implantation site with a mean diameter of 6 mm. Local blood flow and local glucose utilization were measured under light barbiturate anesthesia by quantitative autoradiography in the tumor and peritumoral brain tissue. In solid parts of the tumor, blood flow was 57.8 ± 2.0 ml/100 g/min (mean ± SE), and glucose utilization was 87.2 ± 5.8 μmol/100 g/min, respectively. In necrotic regions, flow and glucose utilization were zero. In peritumoral brain tissue of the ipsilateral hemisphere blood flow was reduced by 13–23%, as compared to homologous regions of the opposite side, the greatest decrease being recorded in the ipsilateral thalamus. Flow in the opposite hemisphere was of the same order of magnitude as in normal control rats. Glucose consumption, in contrast, was distinctly reduced in both hemispheres: in the cortex and putamen, it was 40–50% lower than in normal controls. The following conclusions are drawn: (1) during tumor development the high glucose consumption in the tumor tissue is not coupled to an equal increase in blood flow; (2) peritumoral cerebral blood flow decreases on the ipsilateral but not on the contralateral side, and (3) the metabolic rate of glucose is distinctly inhibited in both hemispheres of tumor-bearing animals. The dissociation between blood flow and metabolism suggests that metabolic inhibition is not the consequence of a diaschitic depression of functional activity.

GLUT2 and hexokinase control proximodistal gradient of intestinal glucose metabolism in the newborn pig

1997 ◽  
Vol 272 (6) ◽  
pp. G1530-G1539 ◽  
Author(s):  
C. Cherbuy ◽  
B. Darcy-Vrillon ◽  
L. Posho ◽  
P. Vaugelade ◽  
M. T. Morel ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Glucose Transporter ◽  
Glucose Utilization ◽  
Specific Effect ◽  
Glucose Consumption ◽  
Utilization Rate ◽  
Glucose Turnover ◽  
Proximal Jejunum ◽  
A Site

We have reported previously that a high glycolytic capacity develops soon after birth in enterocytes isolated from suckling newborn pigs. In the present work, we investigated whether such metabolic changes could affect intestinal glucose utilization in vivo and examined possible variations in glucose metabolism along the small intestine. Glucose utilization by individual tissues was assessed using the 2-deoxyglucose technique. The overall glucose utilization rate was doubled in suckling vs. fasting 2-day-old pigs because of significantly higher rates in all tissues studied, except for the brain. In parallel, enterocytes were isolated from the proximal, medium, or distal jejunoileum of newborn vs. 2-day-old pigs and assessed for their capacity to utilize, transport, and phosphorylate glucose. Intestinal glucose consumption accounted for approximately 15% of glucose turnover rate in suckling vs. 8% in fasting pigs. Moreover, there was a proximal-to-distal gradient of glucose utilization in the intestinal mucosa of suckling pigs. Such a gradient was also evidenced on isolated enterocytes. The stimulation of both hexokinase activity (HK2 isoform) and basolateral glucose transporter (GLUT2), as observed in the proximal jejunum, could account for such a site-specific effect of suckling.

LOCAL CEREBRAL BLOOD FLOW AND GLUCOSE UTILIZATION IN SCHIZOPHRENIA. I. CATATONIC TYPE.

1992 ◽  
Vol 15 ◽  
pp. 258B
Author(s):  
K. Satoh ◽  
M. Narita ◽  
T. Someya ◽  
S. Takahashi ◽  
T. Suzuki ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Local Cerebral Blood Flow

Persistent hypertension does not alter the cerebral blood flow and glucose utilization in young-adult Dahl salt-sensitive rats

2002 ◽  
Vol 197 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
Tetsujiro Yasuda ◽  
Junya Shigematsu ◽  
Shozo Tobimatsu ◽  
Shosuke Takahashi ◽  
Motohiro Kato
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Young Adult ◽  
Glucose Utilization

scholarly journals Local Cerebral Blood Flow with Fentanyl-Induced Seizures

1984 ◽  
Vol 4 (1) ◽  
pp. 88-95 ◽  
Author(s):  
Tsuyoshi Maekawa ◽  
Concezione Tommasino ◽  
Harvey M. Shapiro
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Spike Activity ◽  
Seizure Activity ◽  
Statistical Significance ◽  
Brain Structures ◽  
Local Cerebral Blood Flow ◽  
Progressive Development ◽  
Suppression Phase ◽  
Autoradiographic Technique

Local cerebral blood flow (LCBF) was evaluated with the [14C]iodoantipyrine quantitative autoradiographic technique in 29 brain structures in conscious control rats and during fentanyl-induced electroencephalographic (EEG) spike and/or seizure activity and in the postseizure EEG suppression phase. During spike activity, LCBF increased in all structures; the increase reached statistical significance (p < 0.05) in the superior colliculus, sensorimotor cortex, and pineal body (+ 130%, + 187%, and + 185% from control, respectively). With progressive development of seizure activity, LCBF significantly increased in 24 brain structures (range, +58% to +231% from control). During the postseizure EEG suppression phase, LCBF remained elevated in all structures (+80% to +390% from control). The local cerebrovascular resistance (LCVR) significantly decreased in 10 of 29 structures with the onset of spike activity (range, –24% to –64%), and remained decreased in all brain structures during seizure activity (range, –34% to –67%) and during the EEG suppression phase (range, –24% to –74%). This reduction of LCVR represents a near maximal state of cerebrovasodilation during fentanyl-induced EEG seizure or postseizure suppression activity. The global nature of the LCBF elevation indicates that factors other than local metabolic control are responsible for CBF regulation during local seizure activity.

Cerebral blood flow, glucose use, and CSF ionic regulation in potassium-depleted rats

1988 ◽  
Vol 254 (2) ◽  
pp. H250-H257
Author(s):  
H. Schrock ◽  
W. Kuschinsky
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Local Cerebral Blood Flow ◽  
Arterial Pco2 ◽  
Average Decrease ◽  
K Concentration ◽  
Low K ◽  
The Brain ◽  
K Depletion

Rats were kept on a low-K+ diet for 25 or 70 days. Local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) were measured in 31 different structures of the brain by means of the [14C]iodoantipyrine and [14C]2-deoxy-D-glucose method. After 25 and 70 days of K+ depletion LCBF was decreased significantly in 27 and 30 structures, respectively, the average decrease being 19 and 25%. In contrast, average LCGU was not changed. Cisternal cerebrospinal fluid (CSF) K+ concentration decreased significantly from 2.65 +/- 0.02 mM in controls to 2.55 +/- 0.02 mM and 2.47 +/- 0.02 mM in the two treated groups (P less than 0.01). CSF [HCO3-], pH, and PCO2 were increased in K+-depleted animals. These data show that K+ depletion induces an increase in CSF pH and a decrease in CSF K+ concentration, both of which cause a reduction in cerebral blood flow. The increased CSF PCO2 is secondary to the reduction of blood flow, since brain metabolism and arterial PCO2 remained constant.

Local cerebral blood flow and glucose utilization after blood exchange with a hemoglobin-based O2 carrier in conscious rats

1993 ◽  
Vol 265 (4) ◽  
pp. H1243-H1248 ◽  
Author(s):  
K. Waschke ◽  
H. Schrock ◽  
D. M. Albrecht ◽  
K. van Ackern ◽  
W. Kuschinsky
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Utilization ◽  
Brain Structures ◽  
Control Group ◽  
Local Cerebral Blood Flow ◽  
Conscious Rats ◽  
Cerebral Glucose Utilization ◽  
Arterial Blood ◽  
Blood Exchange

The effects of a blood exchange on cerebral blood flow and glucose utilization were studied. A near to total blood exchange (hematocrit < 3%) was achieved in conscious rats by isovolemic hemodilution. Ultrapurified, polymerized, bovine hemoglobin (UPBHB) served as a blood substitute. Local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) were measured in 34 brain structures of conscious rats by means of the ido[14C]antipyrine and the 2-[14C]-deoxy-D-glucose methods. A group of rats without blood exchange served as control. After blood exchange LCBF increased from 36 to 126% in the different brain structures resulting in a nearly doubled mean cerebral blood flow (+82%). LCGU increased only moderately by 0-24%. Significant increases in LCGU were observed in 16 brain structures. Mean cerebral glucose utilization slightly increased (+14%). The relationship between LCGU and LCBF was found to be tight both in the control group (r = 0.95) as well as after blood replacement (r = 0.94), although it was reset to a higher overall LCBF-to-LCGU ratio. The profound increases in LCBF observed after blood exchange, which were not paralleled by comparable increases in LCGU, might be explained by a reduction of blood viscosity after blood exchange. Additional effects of blood exchange observed in the present study were an increase of mean arterial blood pressure and a decline of heart rate. The results indicate that replacement of blood with the hemoglobin-based oxygen carrier UPBHB appears to meet the cerebral circulatory and metabolic demands of the brain tissue.

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