scholarly journals Non-Invasive Measurement of Cerebral Oxygen Metabolism in the Mouse Brain by Ultra-High Field 17O MR Spectroscopy

2013 ◽  
Vol 33 (12) ◽  
pp. 1846-1849 ◽  
Author(s):  
Weina Cui ◽  
Xiao-Hong Zhu ◽  
Manda L Vollmers ◽  
Emily T Colonna ◽  
Gregor Adriany ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Practical Method ◽  
Oxygen Metabolism ◽  
Resonance Spectroscopy ◽  
Cerebral Oxygen ◽  
Transgenic Mouse Models ◽  
Ultra High Field ◽  
Non Invasive ◽  
Potential Applications ◽  
Invasive Measurement

To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. 17O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO2) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO2 measurements with 17O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.

scholarly journals Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents

2021 ◽  
Vol 6 ◽  
pp. 109
Author(s):  
Tobias C Wood ◽  
Diana Cash ◽  
Eilidh MacNicol ◽  
Camilla Simmons ◽  
Eugene Kim ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Imaging Techniques ◽  
Glucose Consumption ◽  
Oxygen Metabolism ◽  
Oxygen Extraction Fraction ◽  
Strongly Correlated ◽  
Non Invasive ◽  
Cerebral Metabolic Rate ◽  
Invasive Measurement

Malfunctions of oxygen metabolism are suspected to play a key role in a number of neurological and psychiatric disorders, but this hypothesis cannot be properly investigated without an in-vivo non-invasive measurement of brain oxygen consumption. We present a new way to measure the Cerebral Metabolic Rate of Oxygen (CMRO2) by combining two existing magnetic resonance imaging techniques, namely arterial spin-labelling and oxygen extraction fraction mapping. This method was validated by imaging rats under different anaesthetic regimes and was strongly correlated to glucose consumption measured by autoradiography.

scholarly journals Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents

2021 ◽  
Vol 6 ◽  
pp. 109
Author(s):  
Tobias C Wood ◽  
Diana Cash ◽  
Eilidh MacNicol ◽  
Camilla Simmons ◽  
Eugene Kim ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Imaging Techniques ◽  
Glucose Consumption ◽  
Oxygen Metabolism ◽  
Oxygen Extraction Fraction ◽  
Strongly Correlated ◽  
Non Invasive ◽  
Cerebral Metabolic Rate ◽  
Invasive Measurement

Malfunctions of oxygen metabolism are suspected to play a key role in a number of neurological and psychiatric disorders, but this hypothesis cannot be properly investigated without an in-vivo non-invasive measurement of brain oxygen consumption. We present a new way to measure the Cerebral Metabolic Rate of Oxygen (CMRO2) by combining two existing magnetic resonance imaging techniques, namely arterial spin-labelling and oxygen extraction fraction mapping. This method was validated by imaging rats under different anaesthetic regimes and was strongly correlated to glucose consumption measured by autoradiography.

Development of diffuse correlation techniques for non-invasive measurement of cerebral blood flow and oxygen metabolism in rats

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S413-S413
Author(s):  
Turgut Durduran ◽  
Guoqiang Yu ◽  
Chao Zhou ◽  
Daisuke Furuya ◽  
Joseph P Culver ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Oxygen Metabolism ◽  
Non Invasive ◽  
Correlation Techniques ◽  
Invasive Measurement

Simultaneous, non-invasive measurement of local tissue hemodynamics, oxygen metabolism and gold nanorod concentration in vivo

2016 ◽  
Author(s):  
Jordi Morales-Dalmau ◽  
Miguel Mireles ◽  
Johannes Johansson ◽  
Mar Martínez-Lozano ◽  
Clara Vilches ◽  
...  
Keyword(s):  
Oxygen Metabolism ◽  
Gold Nanorod ◽  
Local Tissue ◽  
Non Invasive ◽  
Invasive Measurement

scholarly journals Imaging toxin-induced neuroinflammation in mice using hyperpolarized13C magnetic resonance spectroscopy

2019 ◽  
Author(s):  
Lydia M Le Page ◽  
Caroline Guglielmetti ◽  
Chloé Najac ◽  
Brice Tiret ◽  
Myriam M Chaumeil
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Mouse Brain ◽  
Lactate Production ◽  
Contralateral Side ◽  
Resonance Spectroscopy ◽  
Activated Microglia ◽  
Non Invasive ◽  
Induced Changes

AbstractLipopolysaccharide (LPS) is a commonly used agent for induction of neuroinflammation in preclinical studies. Upon injection, LPS causes activation of microglia and astrocytes, whose metabolism alters to favor glycolysis. Assessingin vivoneuroinflammation and its modulation following therapy remains challenging, and new non-invasive methods allowing for longitudinal monitoring would be greatly valuable. Hyperpolarized (HP)13C magnetic resonance spectroscopy (MRS) is a promising technique for assessingin vivometabolism. In addition to applications in oncology, the most commonly used probe of [1-13C] pyruvate has shown potential in assessing neuroinflammation-linked metabolism in mouse models of multiple sclerosis and traumatic brain injury. Here, we wished to investigate LPS-induced neuroinflammatory changes using HP [1-13C] pyruvate and HP13C urea.2D chemical shift imaging following simultaneous intravenous injection of HP [1-13C] pyruvate and HP13C urea was performed at baseline (day 0), day 3 and day 7 post intracranial injection of LPS (n=6) or saline (n=5). Immunofluorescence (IF) analyses were performed for Iba1 (resting and activated microglia/macrophages), GFAP (resting and reactive astrocytes) and CD68 (activated microglia/macrophages).A significant increase in HP [1-13C] lactate production was observed in the injected (ipsilateral) side at 3 and 7 days of the LPS-treated mouse brain, but not in either the contralateral side or saline-injected animals. HP13C lactate/pyruvate ratio, without and with normalization to urea, was also significantly increased in the ipsilateral LPS-injected brain at 7 days compared to baseline. IF analyses showed a significant increase in CD68 and GFAP at 3 days, followed by increased numbers of Iba1 and GFAP positive cells at 7 days post-LPS injection.In conclusion, we can detect LPS-induced changes in the mouse brain using HP13C MRS, in alignment with increased numbers of microglia/macrophages and astrocytes. This study demonstrates that HP13C spectroscopy holds much potential for providing non-invasive information on neuroinflammation.

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