Csnk1e Is a Genetic Regulator of Sensitivity to Psychostimulants and Opioids

Intramuscular fat (IMF) deposition is one of the most important factors to affect meat quality in livestock and induce insulin resistance and adverse metabolic phenotypes for humans. However, the key regulators involved in this process remain largely unknown. Although liver kinase B1 (LKB1) was reported to participate in the development of skeletal muscles and classical adipose tissues. Due to the specific autonomic location of intramuscular adipocytes, deposited between or within muscle bundles, the exact roles of LKB1 in IMF deposition need further verified. Here, we cloned the goat LKB1 coding sequence with 1,317 bp, encoding a 438 amino acid peptide. LKB1 was extensively expressed in detected tissues and displayed a trend from decline to rise during intramuscular adipogenesis. Functionally, knockdown of LKB1 by two individual siRNAs enhanced the intramuscular preadipocytes differentiation, accompanied by promoting lipid accumulation and inducing adipogenic transcriptional factors and triglyceride synthesis-related genes expression. Conversely, overexpression of LKB1 restrained these biological signatures. To further explore the mechanisms, the RNA-seq technique was performed to compare the difference between siLKB1 and the control group. There were 1,043 differential expression genes (DEGs) were screened, i.e., 425 upregulated genes and 618 downregulated genes in the siLKB1 group. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis predicted that the DEGs were mainly enriched in the focal adhesion pathway and its classical downstream signal, the PI3K-Akt signaling pathway. Specifically, knockdown of LKB1 increased the mRNA level of focal adhesion kinase (FAK) and vice versa in LKB1-overexpressed cells, a key component of the activated focal adhesion pathway. Convincingly, blocking this pathway by a specific FAK inhibitor (PF573228) rescued the observed phenotypes in LKB1 knockdown adipocytes. In conclusion, LKB1 inhibited goat intramuscular adipogenesis through the focal adhesion pathway. This work expanded the genetic regulator networks of IMF deposition and provided theoretical support for improving human health and meat quality from the aspect of IMF deposition.

Download Full-text

The functionally important IL-10 promoter polymorphism (-1082G->A) is not a major genetic regulator in recurrent spontaneous abortions

MHR Basic science of reproductive medicine ◽

10.1093/molehr/7.2.201 ◽

2001 ◽

Vol 7 (2) ◽

pp. 201-203 ◽

Cited By ~ 36

Author(s):

J. Karhukorpi

Keyword(s):

Promoter Polymorphism ◽

Spontaneous Abortions ◽

Recurrent Spontaneous Abortions ◽

Genetic Regulator

Download Full-text

Se(VI) Reduction and the Precipitation of Se(0) by the Facultative Bacterium Enterobacter cloacae SLD1a-1 Are Regulated by FNR

Applied and Environmental Microbiology ◽

10.1128/aem.02542-06 ◽

2007 ◽

Vol 73 (6) ◽

pp. 1914-1920 ◽

Cited By ~ 51

Author(s):

N. Yee ◽

J. Ma ◽

A. Dalia ◽

T. Boonfueng ◽

D. Y. Kobayashi

Keyword(s):

Reductase Activity ◽

Regulatory Gene ◽

Enterobacter Cloacae ◽

Elemental Selenium ◽

Wild Type ◽

E Coli ◽

First Order ◽

Mutant Strains ◽

Reaction Constants ◽

Genetic Regulator

ABSTRACT The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global anaerobic regulatory gene fnr (fumarate nitrate reduction regulator) from E. cloacae in the non-Se-reducing strain Escherichia coli S17-1 activated the ability to reduce Se(VI) and precipitate insoluble Se(0) particles. Se(VI) reduction by E. coli S17-1 containing the fnr gene occurred at rates similar to those for E. cloacae, with first-order reaction constants of k = 2.07 × 10−2 h−1 and k = 3.36 × 10−2 h−1, respectively, and produced elemental selenium particles with identical morphologies and short-range atomic orders. Mutation of the fnr gene in E. cloacae SLD1a-1 resulted in derivative strains that were deficient in selenate reductase activity and unable to precipitate elemental selenium. Complementation by the wild-type fnr sequence restored the ability of mutant strains to reduce Se(VI). Our findings suggest that Se(VI) reduction and the precipitation of Se(0) by facultative anaerobes are regulated by oxygen-sensing transcription factors and occur under suboxic conditions.

Download Full-text

ILIRN is a prominent genetic regulator of interleukin-1? release

Arthritis & Rheumatism ◽

10.1002/art.20756 ◽

2005 ◽

Vol 52 (1) ◽

pp. 361-362 ◽

Cited By ~ 3

Author(s):

Joannis Vamvakopoulos

Keyword(s):

Interleukin 1 ◽

Genetic Regulator

Download Full-text

daughterlesscoordinates somatic cell proliferation, differentiation and germline cyst survival during follicle formation inDrosophila

Development ◽

10.1242/dev.129.13.3255 ◽

2002 ◽

Vol 129 (13) ◽

pp. 3255-3267 ◽

Cited By ~ 4

Author(s):

John E. Smith ◽

Craig A. Cummings ◽

Claire Cronmiller

Keyword(s):

Cell Proliferation ◽

Somatic Cell ◽

Genetic Interaction ◽

Somatic Cells ◽

Loss Of Function ◽

Convergence And Extension ◽

Germline Cyst ◽

Abundance And Diversity ◽

Stem Cell Populations ◽

Genetic Regulator

During Drosophila oogenesis two distinct stem cell populations produce either germline cysts or the somatic cells that surround each cyst and separate each formed follicle. From analyzing daughterless (da) loss-of-function, overexpression and genetic interaction phenotypes, we have identified several specific requirements for da+ in somatic cells during follicle formation. First, da is a critical regulator of somatic cell proliferation. Also, da is required for the complete differentiation of polar and stalk cells, and elevated da levels can even drive the convergence and extension that is characteristic of interfollicular stalks. Finally, da is a genetic regulator of an early checkpoint for germline cyst progression: Loss of da function inhibits normally occurring apoptosis of germline cysts at the region 2a/2b boundary of the germarium, while da overexpression leads to postmitotic cyst degradation. Collectively, these da functions govern the abundance and diversity of somatic cells as they coordinate with germline cysts to form functional follicles.

Download Full-text