scholarly journals Eliminating viscosity bias in lateral flow tests

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Daniel M. Kainz ◽  
Bastian J. Breiner ◽  
Susanna M. Früh ◽  
Tobias Hutzenlaub ◽  
Roland Zengerle ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Nitrocellulose Membrane ◽  
Widespread Application ◽  
Significant Challenge ◽  
Microfluidic Flow ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Lateral Flow Tests ◽  
Viscosity Dependence

AbstractDespite the widespread application of point-of-care lateral flow tests, the viscosity dependence of these assay results remains a significant challenge. Here, we employ centrifugal microfluidic flow control through the nitrocellulose membrane of the strip to eliminate the viscosity bias. The key feature is the balancing of the sample flow into the cassette of the lateral flow test with the air flow out of the cassette. A viscosity-independent flow rate of 3.01 ± 0.18 µl/min (±6%) is demonstrated for samples with viscosities ranging from 1.1 mPas to 24 mPas, a factor greater than 20. In a model human IgG lateral flow assay, signal-intensity shifts caused by varying the sample viscosity from 1.1 mPas to 2.3 mPas could be reduced by more than 84%.

scholarly journals Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Miroslav Pohanka
Keyword(s):  
Quality Of Life ◽  
Point Of Care ◽  
Lateral Flow ◽  
Improve Quality ◽  
New Materials ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Lateral Flow Tests

Analytical devices for point-of-care diagnoses are highly desired and would improve quality of life when first diagnoses are made early and pathologies are recognized soon. Lateral flow tests (LFTs) are such tools that can be easily performed without specific equipment, skills, or experiences. This review is focused on the use of LFT in point-of-care diagnoses. The principle of the assay is explained, and new materials like nanoparticles for labeling, new recognition molecules for interaction with an analyte, and new additional instrumentation like signal scaling by a smartphone camera are described and discussed. Advantages of the LFT devices as well as their limitations are described and discussed here considering actual papers that are properly cited.

Diagnostic ability of salivary matrix metalloproteinase‐9 lateral flow test point‐of‐care test for periodontitis

2020 ◽  
Vol 47 (11) ◽  
pp. 1354-1361
Author(s):  
Hyun‐Duck Kim ◽  
Chang‐Soo Lee ◽  
Hyun‐Jae Cho ◽  
Sumin Jeon ◽  
Young‐Nim Choi ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Point Of Care ◽  
Test Point ◽  
Matrix Metalloproteinase 9 ◽  
Lateral Flow ◽  
Diagnostic Ability ◽  
Point Of Care Test ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Metalloproteinase 9

scholarly journals Analysis of free chlorine in aqueous solution at very low concentration with lateral flow tests

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
K. Uta Schwenke ◽  
Dieter Spiehl ◽  
Marcel Krauße ◽  
Laura Riedler ◽  
Anna Ruppenthal ◽  
...  
Keyword(s):  
Reaction Zone ◽  
Sample Volume ◽  
Lateral Flow ◽  
Free Chlorine ◽  
Test Strips ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Lateral Flow Tests ◽  
User Friendly ◽  
Dip Test

AbstractTest strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out. Their sensitivity is mainly determined by the reactivity of the test dyes on the reaction zone and is not sufficient for some applications. The detection limit of commercially available free chlorine test strips, for example, is at present not low enough to confirm the absence of this analyte as disinfectant in rinsing solutions after disinfection or to control required residual amounts of chlorine in drinking water. Therefore, we developed a user-friendly lateral flow test which is capable to detect very low amounts of free chlorine. The latter relies on a larger sample volume passing the reaction zone as compared to simple dip test strips. An amount of as low as 0.05 ppm chlorine can, however, only be detected if oxidation stable flow test substrates are used. The eventually developed flow test reaches a 10x higher sensitivity than a commercial dip test. The result is obtained within 4–5 min flow time, whereby no action is required by the user during this analysis time.

scholarly journals A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1051-1059 ◽  
Author(s):  
Andrew S. Paterson ◽  
Balakrishnan Raja ◽  
Vinay Mandadi ◽  
Blane Townsend ◽  
Miles Lee ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Test Strip ◽  
Lateral Flow ◽  
Point Of Care Testing ◽  
Flow Test ◽  
Highly Sensitive ◽  
Lateral Flow Test ◽  
Gated Imaging

Time-gated imaging on a smartphone of a lateral flow test strip run with persistent luminescent nanophosphors.

scholarly journals Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26493-26501
Author(s):  
Xue Wang ◽  
Chao-Hua Xue ◽  
Dong Yang ◽  
Shun-Tian Jia ◽  
Ya-Ru Ding ◽  
...  
Keyword(s):  
Detection Limit ◽  
Sensitivity Enhancement ◽  
Lateral Flow ◽  
Fibrous Membrane ◽  
Nitrocellulose Membrane ◽  
Test Strips ◽  
Flow Test ◽  
New Type ◽  
Lateral Flow Test

We constructed a new type of ICT strip by replacing the conventional nitrocellulose membrane with an electrospin-coated nitrocellulose (ENC) fibrous membrane, and the ICT strip could obtain an HCG detection limit of 0.22 mIU mL−1, and an LH detection limit of 0.36 mIU mL−1.

scholarly journals Controlling Capillary Flow Rate on Lateral Flow Test Substrates by Tape

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 562
Author(s):  
Zhiqing Xiao ◽  
Yuqian Yang ◽  
Xingwei Zhang ◽  
Weijin Guo
Keyword(s):  
Flow Rate ◽  
Rate Control ◽  
Capillary Flow ◽  
Lateral Flow ◽  
Average Flow Rate ◽  
Average Flow ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Lateral Flow Tests ◽  
Flow Rate Control

Controlling capillary flow rate of sample liquid is of high interest for lateral flow tests, since the flow rate can affect the dissolution and mixing of the immunoreagents and the efficiency of immunoreactions. Here we develop a facile method to adjust the capillary flow rate on lateral flow test substrates by using tape to cover the surface of substrates. We test this method on the traditional lateral flow test substrate—nitrocellulose and a novel lateral flow test substrate—synthetic paper, which is a porous media made by interlocked off-stoichiometry thiol-ene (OSTE) micropillars. We found that after the surface was covered by tape, the average flow rate decreased to 61% of the original flow rate on nitrocellulose, while the average flow rate increased to at least 320% of the original flow rate on synthetic paper. More interesting, besides the increase of flow rate, the volume capacity of synthetic paper also increases after covered by tape. Furthermore, we investigated the influence of length and position of tape on the capillary flow rate for nitrocellulose. A longer tape will lead to a smaller flow rate. The influence of tape of same length on the flow rate is bigger when the tape is placed closer to the loading pad. These results can help in the flow rate control on lateral flow test substrates, and potentially improve the performance of lateral flow tests.

Integrating micro metal‐air batteries in lateral flow test for point‐of‐care applications

2020 ◽  
Author(s):  
Yifei Wang ◽  
Wending Pan ◽  
Shijing Luo ◽  
Xiaolong Zhao ◽  
Holly Y. H. Kwok ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Diagnostic validation of a rapid and field-applicable PCR-lateral flow test system for point-of-care detection of cyprinid herpesvirus 3 (CyHV-3)

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0241420
Author(s):  
Finn N. Loose ◽  
André Breitbach ◽  
Ivo Bertalan ◽  
Dana Rüster ◽  
Uwe Truyen ◽  
...  
Keyword(s):  
Point Of Care ◽  
Test System ◽  
Lateral Flow ◽  
Cyprinid Herpesvirus 3 ◽  
Flow Test ◽  
Diagnostic Validation ◽  
Lateral Flow Test ◽  
Point Of Care Detection
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