LPS-induced mitochondrial DNA synthesis and release facilitate RAD50-dependent acute lung injury

Abstract Background Mitochondrial DNA (mtDNA) is a critical activator of inflammation. Circulating mtDNA released causes lung injury in experimental models. We hypothesized that elevated plasma mtDNA levels are associated with acute lung injury (ALI) in septic patients. Methods We enrolled 66 patients with sepsis admitted to the Department of Critical Care Medicine of Peking Union Medical College Hospital between January 2019 and October 2019. Respiratory, hemodynamic and bedside echocardiographic parameters were recorded. Plasma mtDNA, procalcitonin, interleukin 6, and interleukin 8 levels were examined. Results Plasma mtDNA levels within 24 h after admission were significantly increased in the group of septic patients with ALI [5.01 (3.38–6.64) vs 4.13 (3.20–5.07) log copies/µL, p 0.0172]. mtDNA levels were independently associated with mortality (hazard ratio, 3.2052; 95% CI 1.1608–8.8500; p 0.0253) and ALI risk (odds ratio 2.7506; 95% CI 1.1647–6.4959; p 0.0210). Patients with high mtDNA levels had worse outcomes, and post hoc tests showed significant differences in 28-day survival rates. Increased mtDNA levels were seen in patients with abdominal infection. Conclusions Increased plasma mtDNA levels within 24 h after admission were significantly associated with ALI incidence and mortality in septic patients.

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Blood Transfusion Products Contain Mitochondrial DNA Damps: A Potential Effector Of Transfusion Related Acute Lung Injury

Journal of Surgical Research ◽

10.1016/j.jss.2013.11.280 ◽

2014 ◽

Vol 186 (2) ◽

pp. 509 ◽

Cited By ~ 1

Author(s):

Y.L. Lee ◽

M. King ◽

M.N. Gillespie ◽

R.P. Gonzalez ◽

S.B. Brevard ◽

...

Keyword(s):

Mitochondrial Dna ◽

Acute Lung Injury ◽

Blood Transfusion ◽

Lung Injury

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Influence of hydroxyurea on the course of germination and growth of rape (Brasica napus L.) seedlings

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1978.011 ◽

2015 ◽

Vol 47 (1–2) ◽

pp. 131-141 ◽

Cited By ~ 1

Author(s):

M. Kuraś ◽

H. Teleżyński

Keyword(s):

Mitochondrial Dna ◽

Root Growth ◽

Dna Synthesis ◽

Elongation Growth ◽

Complete Inhibition ◽

Hypocotyl Growth ◽

Prolonged Incubation ◽

Inhibition Of Growth ◽

Germination And Growth ◽

Mitochondrial Dna Synthesis

The effects of continuous incubation in hydroxyurea (HU) solutions (0.2, 0.4, 0.8 mg/ml) on germination of rape seeds and growth of young seedling axes were studied during 132 hours from initial soaking. Germination turned out to be unaffected by the treatment. Root growth was first increasingly inhibited by the HU concentration tested, but after prolonged incubation a complete arrest of the root growth was noted at all HU concentrations. The elongation growth of hypocotyls was found to be stimulated by a HU 0.2 mg/ml concentration while it was markedly suppressed by 0.4 mg/ml, and completely arrested by 0.8 mg/ml Inhibition of growth of the upright hypocotyl part at higher HU concentration was found to be accompanied by the unbending of the hooked under-cotyledonary part. It is suggested that inhibition of nuclear endomitotic DNA synthesis In elongating hypocotyl cells, suppresses only partially their growth, whereas a complete inhibition of the hypocotyl growth results from arrest of the mitochondrial DNA synthesis.

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